Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GPW70_RS07400 Genome accession   NZ_CP046740
Coordinates   516512..517387 (-) Length   291 a.a.
NCBI ID   WP_000418753.1    Uniprot ID   -
Organism   Vibrio cholerae strain 3566-06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 511512..522387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW70_RS07380 parC 512462..514747 (+) 2286 WP_032473120.1 DNA topoisomerase IV subunit A -
  GPW70_RS07385 yacG 514796..514993 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  GPW70_RS07390 zapD 515135..515875 (-) 741 WP_000207194.1 cell division protein ZapD -
  GPW70_RS07395 coaE 515907..516515 (-) 609 WP_000011553.1 dephospho-CoA kinase -
  GPW70_RS07400 pilD 516512..517387 (-) 876 WP_000418753.1 A24 family peptidase Machinery gene
  GPW70_RS07405 pilC 517445..518671 (-) 1227 WP_000648507.1 type II secretion system F family protein Machinery gene
  GPW70_RS07410 pilB 518714..520402 (-) 1689 WP_001888471.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPW70_RS07415 pilA 520406..520831 (-) 426 WP_032473121.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GPW70_RS07420 nadC 521076..521966 (-) 891 WP_001888470.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32419.67 Da        Isoelectric Point: 7.1618

>NTDB_id=406910 GPW70_RS07400 WP_000418753.1 516512..517387(-) (pilD) [Vibrio cholerae strain 3566-06]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPLLSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFGVALLFFSYVLIAATFIDFDTLLLPDQLTLP
LLWGGIALALLGFSPVSLSDAVIGAMAGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPVIVLLSSVVGV
IFGLIQLRQQKKGIDMAFPFGPYLAIAGWFALLWGDKVIDWYFTTWVGQPL

Nucleotide


Download         Length: 876 bp        

>NTDB_id=406910 GPW70_RS07400 WP_000418753.1 516512..517387(-) (pilD) [Vibrio cholerae strain 3566-06]
ATGGAACTGTTTTATTTCTACCCTTGGCTGTTTCCGGTGCTCGCCACGCTGTTTGGCTTGATTGTCGGCAGCTTTCTCAA
TGTGGTGATCTATCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCGGAATGTGCGGCGAGTTTTCCTGAATATGGCA
TCACGCCACCAGAGGGTAAACTGACCCTCAGTTTGCCGCGTTCTACTTGCCCACACTGCCAAACGCCGATTCGCGTCATT
GATAATATTCCGCTACTGAGTTGGCTGGCGCTGCGCGGTCAATGCTCACACTGTAAAGCGCCGATCAGCGCTCGCTATCC
CTTGATTGAGCTACTCACTGCCCTCATGAGCTTAGTGATTGCCACTCACTTTCCGTTCGGAGTGTTTGGCGTGGCGCTGC
TGTTTTTCAGCTATGTATTGATTGCGGCGACCTTCATCGACTTCGATACCCTATTGCTGCCCGACCAATTAACGCTACCT
TTACTGTGGGGCGGTATTGCACTGGCACTGCTTGGTTTTTCTCCGGTTTCACTCAGTGATGCGGTGATCGGAGCCATGGC
GGGTTATCTCTCGCTGTGGTCTATTTACTGGCTATTTAAACTGCTGACAGGTAAAGAAGGCATGGGTTATGGCGATTTCA
AATTGCTCGCCGCTCTCGGCGCTTGGCTGGGTTGGCAGCAGTTGCCTGTCATCGTACTGCTCTCATCGGTGGTTGGGGTG
ATTTTCGGTTTAATCCAACTGCGCCAGCAGAAAAAAGGTATCGATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
GGGATGGTTTGCCTTGCTGTGGGGCGATAAGGTAATTGATTGGTACTTCACCACTTGGGTAGGACAACCGTTATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(135-244)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

99.656

100

0.997
  pilD Vibrio campbellii strain DS40M4

75.347

98.969

0.746
  pilD Acinetobacter nosocomialis M2

53.676

93.471

0.502
  pilD Acinetobacter baumannii D1279779

53.309

93.471

0.498
  pilD Neisseria gonorrhoeae MS11

44.792

98.969

0.443

Multiple sequence alignment