Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GM695_RS06040 Genome accession   NZ_CP046531
Coordinates   1220598..1221998 (+) Length   466 a.a.
NCBI ID   WP_159629605.1    Uniprot ID   -
Organism   Mannheimia pernigra strain ZY170218     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1215598..1226998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM695_RS06010 (GM695_06000) - 1215704..1216468 (-) 765 WP_159629581.1 TSUP family transporter -
  GM695_RS06015 (GM695_06005) proB 1216468..1217559 (-) 1092 WP_159629587.1 glutamate 5-kinase -
  GM695_RS06020 (GM695_06010) - 1217673..1218167 (+) 495 WP_159629590.1 dihydrofolate reductase -
  GM695_RS06025 (GM695_06015) - 1218295..1218528 (+) 234 WP_159629593.1 YgjV family protein -
  GM695_RS06030 (GM695_06020) radA 1218567..1219946 (-) 1380 WP_159629596.1 DNA repair protein RadA Machinery gene
  GM695_RS06035 (GM695_06025) pilA 1220127..1220576 (+) 450 WP_159629603.1 prepilin peptidase-dependent pilin Machinery gene
  GM695_RS06040 (GM695_06030) pilB 1220598..1221998 (+) 1401 WP_159629605.1 GspE/PulE family protein Machinery gene
  GM695_RS06045 (GM695_06035) pilC 1221982..1223175 (+) 1194 WP_159629607.1 type II secretion system F family protein Machinery gene
  GM695_RS06050 (GM695_06040) - 1223178..1223834 (+) 657 WP_159629610.1 prepilin peptidase -
  GM695_RS06055 (GM695_06045) coaE 1223866..1224504 (+) 639 WP_159629613.1 dephospho-CoA kinase -
  GM695_RS06060 (GM695_06050) yacG 1224486..1224671 (+) 186 WP_159629615.1 DNA gyrase inhibitor YacG -
  GM695_RS06065 (GM695_06055) holA 1224672..1225694 (-) 1023 WP_159629616.1 DNA polymerase III subunit delta -
  GM695_RS06070 (GM695_06060) lptE 1225735..1226223 (-) 489 WP_159629619.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 466 a.a.        Molecular weight: 52660.42 Da        Isoelectric Point: 5.7988

>NTDB_id=405158 GM695_RS06040 WP_159629605.1 1220598..1221998(+) (pilB) [Mannheimia pernigra strain ZY170218]
MAYSVTDLNSQQIIEISAEQWAKNGNEKQILLRYLAVPLRETESQLWLAIDDMQNLSACEIFAFIHHKQIEPVLISSDEL
KFLLNALSPEQSSLQSEIYEDNAAYSLSNAEQVDRNDPIIQILDNLFKFCLKNNASDIHLEPQKERLLIRLRIDGVLHLY
KTFPQNVSNRLISRIKLLAKLDISETRLPQDGQFHFKTILAETLDFRVSTLPTHFGEKVVLRIQKNKPINLDFLELGFTL
SQKEKLLNALNQPQGLILVTGPTGSGKSITLYSALSHLNSNEKHILTAEDPIEIEIEGIIQTQVNRGIHLDFSQLLRTFL
RQDPDIIMLGEIRDEESAEIALRAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYELKNTLLLVVAQRLLRRLCTTCGGI
GCTHCYQGYKGRIGVYQLLSRTAKNFEKTTACLDFETLAESAKEKLVEGKTDQNEILRVLGNGENL

Nucleotide


Download         Length: 1401 bp        

>NTDB_id=405158 GM695_RS06040 WP_159629605.1 1220598..1221998(+) (pilB) [Mannheimia pernigra strain ZY170218]
ATGGCGTATTCAGTTACCGATCTTAACAGCCAGCAAATCATTGAAATTTCTGCCGAACAATGGGCAAAAAACGGCAATGA
AAAGCAGATTTTGTTGCGATATTTAGCCGTTCCGTTGCGAGAAACTGAATCACAATTATGGTTAGCGATTGATGATATGC
AAAACTTGAGTGCGTGTGAGATCTTTGCTTTTATTCATCATAAGCAGATTGAGCCTGTTTTGATTTCCTCTGATGAGTTG
AAATTTTTACTTAATGCCCTTTCACCTGAGCAGAGTAGCTTACAGAGTGAAATTTATGAGGATAATGCCGCTTATTCGTT
ATCAAATGCAGAGCAGGTAGATAGAAACGATCCGATTATTCAAATTTTAGATAATCTCTTTAAATTTTGCTTAAAAAATA
ATGCCTCAGACATCCATTTGGAGCCTCAGAAAGAGAGATTACTCATTCGGCTAAGAATTGATGGAGTTTTACATCTTTAT
AAAACGTTTCCTCAGAATGTATCAAACCGACTGATTTCTCGAATTAAATTACTAGCGAAACTAGATATTAGTGAAACCCG
TTTGCCTCAAGATGGGCAATTTCATTTTAAAACGATTTTAGCGGAAACCTTAGATTTCCGTGTTTCAACCTTGCCCACGC
ATTTTGGTGAAAAAGTGGTACTTCGGATTCAAAAAAATAAGCCAATTAATTTGGATTTTTTAGAGTTAGGATTTACCCTG
AGCCAGAAAGAAAAATTGCTCAATGCACTGAATCAACCGCAAGGCTTGATTCTGGTAACAGGGCCGACAGGTAGTGGCAA
AAGTATCACGCTTTATAGTGCATTAAGTCATTTAAATAGCAATGAAAAACATATTTTAACTGCGGAAGATCCAATTGAAA
TTGAGATAGAGGGTATTATTCAAACTCAAGTAAATCGTGGTATTCATCTTGATTTTAGCCAACTACTGAGAACCTTCCTT
CGACAAGATCCTGACATCATTATGCTAGGTGAGATCCGTGATGAGGAGAGTGCAGAAATTGCCTTAAGAGCGGCACAAAC
TGGGCATTTGGTTCTTTCGACATTACATACTAATGATGCACCCTCTGCTATAGAGCGATTATTACAACTAGGCATTAAGG
AATATGAACTAAAAAATACATTATTACTCGTCGTTGCTCAACGTCTATTAAGGCGGCTTTGTACAACGTGCGGAGGAATT
GGGTGTACGCATTGCTATCAAGGCTACAAAGGTCGAATTGGGGTTTATCAACTCTTGAGCAGAACTGCAAAAAATTTTGA
AAAAACAACTGCTTGTTTAGATTTTGAAACCTTAGCGGAGAGTGCAAAAGAAAAATTAGTGGAAGGAAAAACAGATCAGA
ATGAAATTTTGAGGGTATTAGGAAATGGCGAAAATCTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(131-392)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

70.435

98.712

0.695
  pilB Haemophilus influenzae 86-028NP

57.269

97.425

0.558
  pilB Haemophilus influenzae Rd KW20

57.048

97.425

0.556
  pilB Vibrio campbellii strain DS40M4

41.079

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.956

100

0.423
  pilB Vibrio cholerae strain A1552

40.041

100

0.414
  pilB Legionella pneumophila strain ERS1305867

39.014

100

0.408
  pilB Acinetobacter baylyi ADP1

37.219

100

0.391
  pilB Acinetobacter baumannii D1279779

37.014

100

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.288

100

0.378
  ctsE Campylobacter jejuni subsp. jejuni 81-176

41.727

89.485

0.373

Multiple sequence alignment