Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GM695_RS06030 Genome accession   NZ_CP046531
Coordinates   1218567..1219946 (-) Length   459 a.a.
NCBI ID   WP_159629596.1    Uniprot ID   -
Organism   Mannheimia pernigra strain ZY170218     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1213567..1224946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM695_RS06000 (GM695_05990) - 1213795..1215033 (-) 1239 WP_159629576.1 multifunctional CCA addition/repair protein -
  GM695_RS06005 (GM695_05995) nth 1215066..1215701 (-) 636 WP_159629578.1 endonuclease III -
  GM695_RS06010 (GM695_06000) - 1215704..1216468 (-) 765 WP_159629581.1 TSUP family transporter -
  GM695_RS06015 (GM695_06005) proB 1216468..1217559 (-) 1092 WP_159629587.1 glutamate 5-kinase -
  GM695_RS06020 (GM695_06010) - 1217673..1218167 (+) 495 WP_159629590.1 dihydrofolate reductase -
  GM695_RS06025 (GM695_06015) - 1218295..1218528 (+) 234 WP_159629593.1 YgjV family protein -
  GM695_RS06030 (GM695_06020) radA 1218567..1219946 (-) 1380 WP_159629596.1 DNA repair protein RadA Machinery gene
  GM695_RS06035 (GM695_06025) pilA 1220127..1220576 (+) 450 WP_159629603.1 prepilin peptidase-dependent pilin Machinery gene
  GM695_RS06040 (GM695_06030) pilB 1220598..1221998 (+) 1401 WP_159629605.1 GspE/PulE family protein Machinery gene
  GM695_RS06045 (GM695_06035) pilC 1221982..1223175 (+) 1194 WP_159629607.1 type II secretion system F family protein Machinery gene
  GM695_RS06050 (GM695_06040) - 1223178..1223834 (+) 657 WP_159629610.1 prepilin peptidase -
  GM695_RS06055 (GM695_06045) coaE 1223866..1224504 (+) 639 WP_159629613.1 dephospho-CoA kinase -
  GM695_RS06060 (GM695_06050) yacG 1224486..1224671 (+) 186 WP_159629615.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49831.39 Da        Isoelectric Point: 7.9649

>NTDB_id=405156 GM695_RS06030 WP_159629596.1 1218567..1219946(-) (radA) [Mannheimia pernigra strain ZY170218]
MAKAPKTAYVCNDCGAEYSRWMGQCRECKAWNTISEVRLISSKETAKSDRFSGYAGETSGKVQALSDISLQEVPRFSSGF
NELDRVLGGGVVPGSAILIGGHPGAGKSTLLLQVMCGLAQNIPTLYVTGEESLQQVAMRANRLGLPAQNLKMLSETSVEH
ICNIADQEKPKIMVIDSIQVMHLSDIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVGHVTKDGTLAGPKVLEHAIDASL
LLEGESDSRFRTLRSHKNRFGAVNELGVFAMTEQGLKEVKNPSAIFLSRSEEQTPGSSVMVLWEGTRPLLVEIQALVDHS
MLANPRRVAVGLDHNRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALMSSFRNRPLPQDLVIFGEVG
LAGEIRPVTSGQERISEAAKHGFRRAIIPHGNAPKKPIKDMDVFTVKKLSDALDILYNL

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=405156 GM695_RS06030 WP_159629596.1 1218567..1219946(-) (radA) [Mannheimia pernigra strain ZY170218]
ATGGCAAAAGCACCTAAAACCGCCTATGTTTGTAATGATTGTGGGGCGGAATACTCCCGTTGGATGGGGCAATGCCGAGA
ATGTAAGGCTTGGAATACCATCAGCGAAGTTAGATTGATTTCGAGTAAAGAAACCGCAAAATCTGACCGCTTTAGTGGCT
ATGCAGGGGAAACCTCAGGCAAAGTTCAGGCTCTTTCCGATATTAGCTTGCAAGAAGTGCCTCGCTTTAGTAGTGGTTTT
AATGAGCTAGATCGTGTGCTAGGTGGGGGGGTTGTACCAGGTTCTGCCATTCTTATTGGCGGACACCCTGGTGCAGGCAA
AAGTACGCTTTTACTCCAAGTAATGTGTGGCTTAGCTCAAAATATACCTACTCTTTATGTTACAGGTGAGGAATCTTTGC
AGCAAGTGGCAATGCGTGCTAACCGTTTAGGTTTACCTGCACAAAATCTTAAAATGCTATCTGAAACCTCCGTTGAGCAT
ATTTGCAATATTGCCGATCAAGAAAAACCTAAAATTATGGTAATCGATTCTATTCAAGTGATGCACTTATCCGATATTCA
ATCATCTCCAGGAAGCGTTTCACAGGTTCGAGAATGTGCTTCATTTTTAACTCGTTATGCTAAAACCCGCCAAGTCGCCA
TTATTATGGTAGGGCACGTTACTAAAGATGGGACACTTGCTGGCCCAAAAGTATTGGAACACGCTATTGATGCTTCATTA
TTACTTGAAGGGGAGTCCGATTCTCGATTCCGCACTTTGCGTAGCCATAAAAACCGTTTTGGTGCAGTAAATGAGTTAGG
TGTATTCGCAATGACTGAGCAGGGCTTGAAAGAGGTTAAAAATCCCTCTGCAATTTTCCTTAGCCGTAGCGAAGAACAAA
CGCCTGGTAGCTCTGTAATGGTATTGTGGGAAGGAACTCGCCCGCTTCTAGTTGAAATTCAGGCTCTAGTCGATCATTCA
ATGCTAGCAAATCCTCGCCGTGTCGCCGTTGGTTTAGATCATAACCGTTTATCGCTACTGCTTGCAGTTTTACATCGACA
CGGCGGACTACAAATGTCAGATCAAGACGTGTTTGTCAACGTCGTAGGCGGTGTGAAAGTAACCGAAACCAGTGCCGATC
TGGCTCTGATCTTGGCTTTAATGTCTAGCTTTCGCAATCGCCCATTACCACAAGATTTAGTTATTTTTGGCGAGGTGGGA
TTAGCAGGAGAAATTCGCCCTGTGACTAGCGGACAAGAACGAATTAGTGAAGCAGCAAAACACGGCTTTAGGCGAGCCAT
CATTCCCCACGGTAATGCGCCTAAAAAGCCGATTAAAGATATGGACGTTTTTACTGTAAAAAAACTAAGTGATGCGTTAG
ATATTCTTTATAATTTATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(84-224)

Subunit ChlI of Mg-chelatase (ChlI)

(347-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.359

99.564

0.481
  radA Streptococcus mitis NCTC 12261

43.833

98.911

0.434
  radA Streptococcus mitis SK321

43.612

98.911

0.431
  radA Streptococcus pneumoniae Rx1

42.919

100

0.429
  radA Streptococcus pneumoniae D39

42.919

100

0.429
  radA Streptococcus pneumoniae R6

42.919

100

0.429
  radA Streptococcus pneumoniae TIGR4

42.919

100

0.429

Multiple sequence alignment