Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ALIDE2_RS04350 Genome accession   NC_015422
Coordinates   913339..914556 (-) Length   405 a.a.
NCBI ID   WP_013517808.1    Uniprot ID   A0A858ZQC9
Organism   Alicycliphilus denitrificans K601     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 908339..919556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALIDE2_RS04320 (Alide2_0867) - 909423..910295 (+) 873 WP_013517802.1 ATP-binding protein -
  ALIDE2_RS04325 (Alide2_0868) - 910292..910717 (+) 426 WP_013517803.1 NUDIX domain-containing protein -
  ALIDE2_RS04330 (Alide2_0869) - 910780..910986 (-) 207 WP_013517804.1 DNA gyrase inhibitor YacG -
  ALIDE2_RS04335 (Alide2_0870) zapD 910988..911743 (-) 756 WP_013721477.1 cell division protein ZapD -
  ALIDE2_RS04340 (Alide2_0871) coaE 911826..912440 (-) 615 WP_013517806.1 dephospho-CoA kinase -
  ALIDE2_RS04345 (Alide2_0872) pilD 912461..913339 (-) 879 WP_013517807.1 prepilin peptidase Machinery gene
  ALIDE2_RS04350 (Alide2_0873) pilC 913339..914556 (-) 1218 WP_013517808.1 type II secretion system F family protein Machinery gene
  ALIDE2_RS04355 (Alide2_0874) pilB 914578..916317 (-) 1740 WP_013721478.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ALIDE2_RS04360 (Alide2_0875) - 916693..917784 (+) 1092 WP_013721479.1 site-specific integrase -
  ALIDE2_RS04365 (Alide2_0876) - 918106..918597 (+) 492 WP_013721480.1 helix-turn-helix domain-containing protein -
  ALIDE2_RS04370 (Alide2_0877) - 918594..919250 (+) 657 WP_013721481.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44279.04 Da        Isoelectric Point: 9.3572

>NTDB_id=40428 ALIDE2_RS04350 WP_013517808.1 913339..914556(-) (pilC) [Alicycliphilus denitrificans K601]
MATIASRDIKDFVFEWEGKDRSGKVVRGEVRASGENQVKATLRRQGVLPTKIKKRRASGGKKIKPKDIALFTRQMATMMK
AGVPLLQSFDIVGRGNTNASVTKLLNDIRADVETGTSLNAAFRKYPMYFDSLYCNLVEAGEAAGILEALLDRLATYMEKT
EAIKSKIRSALMYPISVLVVAFVVVTVIMIFVIPAFKEVFTSFGADLPAPTLLVMAISEFFVAYWWLIFGAIGGGFYFFM
QAWKRSEKMQRTMDRFLLKMPIFGALIEKSCVARWTRTLSTMFAAGVPLVEALDSVGGASGNSVYAVATDRIQQEVSTGT
SLTAAMGNANVFPSMVLQMCAIGEESGSIDHMLGKAADFYEAEVDEMVAGLSSLMEPIIIVFLGTLIGGIVVSMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=40428 ALIDE2_RS04350 WP_013517808.1 913339..914556(-) (pilC) [Alicycliphilus denitrificans K601]
ATGGCGACCATCGCATCAAGGGACATCAAGGACTTCGTCTTCGAATGGGAGGGCAAGGACCGCAGCGGCAAGGTCGTGCG
AGGCGAGGTCCGGGCCTCCGGAGAGAACCAGGTCAAGGCGACCCTGCGCCGCCAGGGCGTCCTGCCCACCAAGATCAAGA
AGCGCCGGGCGAGCGGGGGCAAGAAGATCAAGCCCAAGGACATCGCCCTGTTCACGCGCCAGATGGCCACGATGATGAAG
GCCGGCGTGCCGCTGCTGCAATCGTTCGACATCGTGGGGCGCGGCAACACCAATGCCAGCGTGACCAAGCTGCTCAACGA
CATCCGCGCCGACGTGGAGACCGGCACATCGCTCAACGCCGCGTTCCGCAAATATCCCATGTACTTCGACAGCCTGTACT
GCAACCTCGTGGAGGCAGGCGAGGCGGCCGGTATCCTGGAGGCGCTGCTGGACCGCCTGGCCACCTACATGGAGAAGACC
GAGGCCATCAAGTCCAAGATCCGCTCCGCCCTGATGTACCCGATTTCGGTGCTCGTCGTCGCATTCGTCGTGGTCACGGT
GATCATGATCTTCGTGATCCCCGCGTTCAAGGAAGTGTTCACATCCTTCGGCGCCGACCTGCCCGCACCCACGCTGCTCG
TGATGGCGATCAGCGAATTCTTCGTCGCCTACTGGTGGCTGATCTTCGGCGCCATCGGGGGGGGCTTCTACTTCTTCATG
CAGGCCTGGAAGCGCAGCGAGAAGATGCAACGCACCATGGACCGCTTCCTGCTGAAGATGCCCATCTTCGGCGCCCTGAT
CGAGAAGTCCTGCGTGGCGCGCTGGACGCGCACCCTGTCGACCATGTTCGCCGCCGGCGTGCCGCTGGTGGAGGCGCTCG
ACTCCGTGGGCGGCGCCTCGGGCAATTCGGTCTATGCCGTGGCCACCGACAGGATCCAGCAGGAGGTCTCCACCGGCACC
AGCCTGACGGCGGCGATGGGCAATGCCAATGTGTTTCCGTCCATGGTGCTGCAGATGTGCGCCATCGGTGAGGAATCGGG
CTCCATCGACCACATGCTGGGCAAGGCGGCCGACTTCTACGAGGCCGAGGTCGACGAGATGGTGGCCGGCCTGTCCAGCC
TCATGGAGCCCATCATCATCGTGTTCCTGGGCACCCTCATCGGCGGCATCGTGGTGTCCATGTACCTGCCCATCTTCAAG
CTGGGGCAAGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A858ZQC9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.568

100

0.546
  pilG Neisseria gonorrhoeae MS11

52.778

97.778

0.516
  pilG Neisseria meningitidis 44/76-A

52.525

97.778

0.514
  pilC Legionella pneumophila strain ERS1305867

50.503

98.272

0.496
  pilC Acinetobacter baylyi ADP1

49.497

98.272

0.486
  pilC Acinetobacter baumannii D1279779

48.866

98.025

0.479
  pilC Vibrio campbellii strain DS40M4

39.855

100

0.407
  pilC Vibrio cholerae strain A1552

40.657

97.778

0.398
  pilC Thermus thermophilus HB27

36.973

99.506

0.368

Multiple sequence alignment