Detailed information NTG0040427

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ALIDE2_RS04345
• Genome accession: NC_015422
• Coordinates: 912461..913339 (-)
• Length: 292 a.a.
• NCBI ID: WP_013517807.1
• Uniprot ID: A0A858ZQU3
• Organism: Alicycliphilus denitrificans K601
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 907461..918339

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ALIDE2_RS04315 (Alide2_0866)	argJ	908139..909368 (+)	1230	WP_013721476.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
ALIDE2_RS04320 (Alide2_0867)	-	909423..910295 (+)	873	WP_013517802.1	ATP-binding protein	-
ALIDE2_RS04325 (Alide2_0868)	-	910292..910717 (+)	426	WP_013517803.1	NUDIX domain-containing protein	-
ALIDE2_RS04330 (Alide2_0869)	-	910780..910986 (-)	207	WP_013517804.1	DNA gyrase inhibitor YacG	-
ALIDE2_RS04335 (Alide2_0870)	zapD	910988..911743 (-)	756	WP_013721477.1	cell division protein ZapD	-
ALIDE2_RS04340 (Alide2_0871)	coaE	911826..912440 (-)	615	WP_013517806.1	dephospho-CoA kinase	-
ALIDE2_RS04345 (Alide2_0872)	pilD	912461..913339 (-)	879	WP_013517807.1	prepilin peptidase	Machinery gene
ALIDE2_RS04350 (Alide2_0873)	pilC	913339..914556 (-)	1218	WP_013517808.1	type II secretion system F family protein	Machinery gene
ALIDE2_RS04355 (Alide2_0874)	pilB	914578..916317 (-)	1740	WP_013721478.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ALIDE2_RS04360 (Alide2_0875)	-	916693..917784 (+)	1092	WP_013721479.1	site-specific integrase	-

Sequence

Protein

• Length: 292 a.a.
• Molecular weight: 31415.02 Da
• Isoelectric Point: 7.8150

>NTDB_id=40427 ALIDE2_RS04345 WP_013517807.1 912461..913339(-) (pilD) [Alicycliphilus denitrificans K601]
MTGSAFADAVLVGVLGLLIGSFLNVVIHRLPRMMERQWAAECAQYAEDAGLPTQGGAAPTEQPFNLLTPRSRCPSCGHEV
RWYENIPVLSYLALRGRCSGCGTRIGVRYPLVELATAALFYACALRWGWSFTTLAWCGFCAALVALALIDWDTTLLPDDI
TLPLLWAGLLASVLRWIDVQPVDAVIGAAAGYVSLWLVYWGFKLATGKEGMGYGDFKLFAALGAWFGWQALVPIVLMASV
IGALVGIAMKFASSLREGKYVPFGPFLAGGGLAAMLWGPARIMRAIFSTLGL

Nucleotide

• Length: 879 bp

>NTDB_id=40427 ALIDE2_RS04345 WP_013517807.1 912461..913339(-) (pilD) [Alicycliphilus denitrificans K601]
ATGACGGGTTCCGCGTTTGCGGATGCCGTGCTCGTCGGCGTCCTGGGCCTGCTGATCGGCAGCTTTCTGAACGTGGTGAT
CCACCGCCTGCCGCGCATGATGGAACGGCAGTGGGCCGCGGAATGCGCGCAGTACGCCGAGGATGCGGGCTTGCCGACAC
AGGGCGGCGCCGCGCCGACCGAGCAGCCCTTCAACCTGCTGACGCCACGCTCGCGCTGTCCCTCCTGCGGGCACGAGGTG
CGCTGGTACGAGAACATTCCCGTGCTCAGCTATCTCGCACTGCGCGGCCGCTGCTCGGGCTGCGGCACGCGCATCGGCGT
GCGCTACCCGCTGGTCGAACTGGCCACCGCCGCCCTGTTCTATGCCTGCGCCCTGCGCTGGGGCTGGTCCTTCACGACGC
TGGCCTGGTGCGGCTTTTGCGCAGCCCTGGTGGCGCTGGCCCTCATCGACTGGGACACGACCCTGCTGCCCGACGACATC
ACCCTGCCCCTGCTGTGGGCCGGGCTGCTGGCCTCCGTGCTGCGCTGGATAGATGTGCAGCCGGTGGACGCGGTGATCGG
CGCGGCCGCGGGCTATGTATCGCTGTGGCTCGTCTACTGGGGCTTCAAGCTCGCCACGGGCAAGGAGGGCATGGGCTATG
GCGACTTCAAGCTGTTCGCGGCCCTGGGCGCATGGTTCGGCTGGCAGGCCCTGGTGCCCATCGTCCTCATGGCGTCGGTC
ATCGGCGCCCTCGTAGGCATCGCCATGAAGTTCGCCAGCAGCCTGCGCGAGGGCAAGTACGTGCCCTTTGGCCCGTTCCT
GGCGGGAGGCGGGCTGGCCGCCATGCTGTGGGGGCCGGCGCGTATCATGCGTGCCATTTTCTCCACCCTGGGGCTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (139-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (14-126)

3D structure

• AlphaFold DB: A0A858ZQU3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.175	97.603	0.49
pilD	Vibrio campbellii strain DS40M4	45.39	96.575	0.438
pilD	Neisseria gonorrhoeae MS11	47.71	89.726	0.428
pilD	Acinetobacter nosocomialis M2	43.223	93.493	0.404
pilD	Acinetobacter baumannii D1279779	43.223	93.493	0.404