Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   GJS34_RS00685 Genome accession   NZ_CP046042
Coordinates   119719..120657 (+) Length   312 a.a.
NCBI ID   WP_038674626.1    Uniprot ID   -
Organism   Streptococcus equi subsp. zooepidemicus strain TN-714097     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 114719..125657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJS34_RS00670 (GJS34_00670) - 115084..116316 (+) 1233 WP_014622136.1 hypothetical protein -
  GJS34_RS00675 (GJS34_00675) - 116889..118892 (+) 2004 WP_154293407.1 DUF1430 domain-containing protein -
  GJS34_RS00680 (GJS34_00680) - 118894..119562 (+) 669 WP_014622139.1 ATP-binding cassette domain-containing protein -
  GJS34_RS00685 (GJS34_00685) comGA/cglA 119719..120657 (+) 939 WP_038674626.1 competence type IV pilus ATPase ComGA Machinery gene
  GJS34_RS00690 (GJS34_00690) comYB 120590..121624 (+) 1035 WP_228269618.1 competence type IV pilus assembly protein ComGB Machinery gene
  GJS34_RS00695 (GJS34_00695) comGC/cglC 121625..121948 (+) 324 WP_014622142.1 competence type IV pilus major pilin ComGC Machinery gene
  GJS34_RS00700 (GJS34_00700) comGD 121926..122351 (+) 426 WP_012678812.1 competence type IV pilus minor pilin ComGD -
  GJS34_RS00705 (GJS34_00705) comGE 122356..122598 (+) 243 WP_012677236.1 competence type IV pilus minor pilin ComGE -
  GJS34_RS00710 (GJS34_00710) comYF 122585..123019 (+) 435 WP_014622144.1 competence type IV pilus minor pilin ComGF Machinery gene
  GJS34_RS10500 comGG 122997..123176 (+) 180 WP_014622145.1 competence type IV pilus minor pilin ComGG -
  GJS34_RS00720 (GJS34_00720) comYH 123421..124374 (+) 954 WP_014622146.1 class I SAM-dependent methyltransferase Machinery gene
  GJS34_RS00725 (GJS34_00725) - 124434..125633 (+) 1200 WP_012678817.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35254.51 Da        Isoelectric Point: 7.1375

>NTDB_id=400562 GJS34_RS00685 WP_038674626.1 119719..120657(+) (comGA/cglA) [Streptococcus equi subsp. zooepidemicus strain TN-714097]
MVQELAKRLIRQAEELQAQDIYILPRGAGYELLMRIGDDRRLIDVCESDRMANLISHFKFVAGMNVGEKRRCQLGACDYD
LDGTKVSLRLSAVGDYQGQESLVIRLLYHHQRQLRYWFDGLERVTSAIGARGLYLFSGPVGSGKTTLMYQLVSDYCKELQ
VISIEDPVEIKNDQLLQLQVNDSIGMTYDNLIKLSLRHRPDVLIIGEIRDTQTARAVIRASLTGAMVFSTVHAKSISGVY
ARLLELGISRAELDNSLAMVAYQRFISGGALIDCAQKEFEHHQASQWNQRIDQLLAEGHLNTKQARLEKIIQ

Nucleotide


Download         Length: 939 bp        

>NTDB_id=400562 GJS34_RS00685 WP_038674626.1 119719..120657(+) (comGA/cglA) [Streptococcus equi subsp. zooepidemicus strain TN-714097]
ATGGTTCAAGAATTAGCCAAGAGGCTTATCAGGCAGGCCGAAGAATTGCAGGCTCAGGATATTTATATACTGCCAAGAGG
GGCAGGCTATGAGCTTTTGATGAGGATAGGTGATGACAGGAGGCTAATAGATGTTTGTGAAAGTGATCGGATGGCTAATC
TTATTAGTCACTTTAAGTTCGTTGCAGGAATGAATGTGGGAGAAAAAAGACGCTGCCAGCTCGGTGCTTGTGACTACGAC
CTTGATGGCACTAAGGTATCTCTACGATTATCAGCTGTGGGTGACTATCAGGGACAGGAGAGCTTGGTTATTCGTCTCTT
GTATCATCATCAACGCCAGCTCAGGTATTGGTTTGATGGTTTAGAGCGAGTCACATCAGCTATTGGAGCCAGAGGCCTTT
ACCTCTTTTCTGGACCGGTTGGATCAGGTAAGACAACCCTGATGTATCAGCTAGTCTCTGACTATTGCAAGGAGCTTCAG
GTGATCAGTATTGAGGATCCTGTGGAAATTAAAAATGATCAGCTCCTGCAATTACAGGTTAACGACAGCATTGGCATGAC
ATACGATAATCTGATTAAGCTTTCCTTGCGTCATCGTCCAGATGTGTTGATTATCGGAGAAATCAGAGATACACAGACTG
CTAGAGCGGTTATTAGGGCTAGCCTGACAGGAGCAATGGTCTTTTCAACCGTCCATGCAAAGAGTATTTCAGGTGTTTAC
GCTAGGCTGTTAGAATTAGGCATTTCTAGGGCAGAATTGGATAATAGTCTAGCAATGGTTGCTTACCAGCGATTTATTAG
TGGAGGTGCTTTAATTGATTGTGCGCAAAAGGAATTTGAACATCATCAAGCCAGCCAGTGGAATCAGAGGATTGATCAGC
TTCTTGCAGAGGGACATCTCAATACCAAGCAGGCAAGGCTTGAAAAAATTATCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

68.71

99.359

0.683
  comYA Streptococcus mutans UA140

63.023

99.679

0.628
  comYA Streptococcus mutans UA159

63.023

99.679

0.628
  comYA Streptococcus gordonii str. Challis substr. CH1

61.29

99.359

0.609
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

61.29

99.359

0.609
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

60.968

99.359

0.606
  comGA/cglA/cilD Streptococcus pneumoniae D39

60.968

99.359

0.606
  comGA/cglA/cilD Streptococcus pneumoniae R6

60.968

99.359

0.606
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

60.968

99.359

0.606
  comGA Lactococcus lactis subsp. cremoris KW2

53.055

99.679

0.529
  comGA Latilactobacillus sakei subsp. sakei 23K

39.661

94.551

0.375

Multiple sequence alignment