Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GII23_RS07280 Genome accession   NZ_CP045858
Coordinates   1574355..1576058 (+) Length   567 a.a.
NCBI ID   WP_165562832.1    Uniprot ID   -
Organism   Stutzerimonas balearica strain EC28     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1569355..1581058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GII23_RS07250 (GII23_07230) - 1569721..1570764 (-) 1044 WP_165562826.1 acyltransferase -
  GII23_RS07255 (GII23_07235) - 1570788..1571672 (-) 885 WP_165562827.1 ABC transporter ATP-binding protein -
  GII23_RS07260 (GII23_07240) - 1571669..1572502 (-) 834 WP_165562828.1 hypothetical protein -
  GII23_RS07265 (GII23_07245) - 1572499..1573299 (-) 801 WP_165562829.1 ABC transporter permease -
  GII23_RS07270 (GII23_07250) - 1573357..1573812 (-) 456 WP_165562830.1 hypothetical protein -
  GII23_RS22155 (GII23_07255) pilA 1573830..1574015 (-) 186 WP_165562831.1 type IV pilin protein Machinery gene
  GII23_RS07280 (GII23_07260) pilB 1574355..1576058 (+) 1704 WP_165562832.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GII23_RS07285 (GII23_07265) pilC 1576061..1577281 (+) 1221 WP_165562833.1 type II secretion system F family protein Machinery gene
  GII23_RS07290 (GII23_07270) pilD 1577287..1578150 (+) 864 WP_165562834.1 A24 family peptidase Machinery gene
  GII23_RS07295 (GII23_07275) coaE 1578282..1578890 (+) 609 WP_165562835.1 dephospho-CoA kinase -
  GII23_RS07300 (GII23_07280) yacG 1578887..1579084 (+) 198 WP_102840588.1 DNA gyrase inhibitor YacG -
  GII23_RS07305 (GII23_07285) - 1579109..1579789 (-) 681 WP_165562836.1 energy-coupling factor ABC transporter permease -
  GII23_RS07310 (GII23_07290) rplM 1580156..1580584 (+) 429 WP_165562837.1 50S ribosomal protein L13 -
  GII23_RS07315 (GII23_07295) rpsI 1580599..1580991 (+) 393 WP_043221690.1 30S ribosomal protein S9 -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62246.27 Da        Isoelectric Point: 5.8005

>NTDB_id=398711 GII23_RS07280 WP_165562832.1 1574355..1576058(+) (pilB) [Stutzerimonas balearica strain EC28]
MNDNAPLGGLARQLVLAGQLDERVALQAQQQASRNQVPLVTWLVQNKLVNGRTLVGVAGEQFGLPFFDLAALDRDAQPRD
LVSEKLVRQHRVLPLQRRGNRLYVAVSDPTNHQAISDIQFNTGLSIEAVLVEDDKLGEAIEKFFESATTALDGLGDADLN
GLDVEISGEVKDDEGPHNEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRFRTDGMLHEVARPPVQLVSRISAR
LKVMASLDIAERRKPQDGRIKMKVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAKMGIDALGYEEDQKELYLTALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPSCKAEVDVPREVLLKEGFP
EARIGTFKLYGPVGCDSCKGGYKGRVGIYEVVKITPSLQRLIMEEGNSIDIALQARKDGFNDLRTSGLVKAMNGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=398711 GII23_RS07280 WP_165562832.1 1574355..1576058(+) (pilB) [Stutzerimonas balearica strain EC28]
ATGAACGACAATGCGCCCCTTGGTGGTTTGGCTCGTCAGCTAGTACTGGCTGGACAGCTTGATGAAAGGGTTGCTCTCCA
AGCTCAGCAGCAGGCCTCGCGCAATCAGGTGCCGCTGGTGACTTGGCTGGTGCAGAACAAGTTGGTGAATGGGCGGACTT
TAGTGGGCGTCGCTGGAGAGCAGTTCGGTTTACCGTTCTTCGATCTTGCTGCGCTGGATAGGGATGCCCAGCCCCGGGAC
TTGGTGAGTGAGAAACTGGTTCGTCAGCATCGGGTGCTGCCGCTGCAGCGACGCGGTAACAGGCTTTATGTGGCGGTCTC
CGACCCGACTAATCATCAAGCGATCAGTGATATCCAGTTCAACACGGGTCTGTCCATCGAGGCGGTGCTGGTGGAGGACG
ACAAGCTTGGCGAGGCGATCGAGAAGTTCTTCGAGTCGGCGACCACTGCGCTTGATGGTCTCGGAGATGCTGATCTTAAC
GGGTTGGATGTCGAAATCAGCGGAGAGGTGAAGGATGACGAAGGGCCTCACAACGAGGCCGATGATGCGCCGGTCGTGCG
CTTCGTCAACAAGATGTTGCTCGATGCGATCAAGGGTGGGTCGTCAGACCTGCACTTCGAGCCCTATGAAAAATCCTATC
GGGTGCGTTTCCGTACCGACGGCATGTTGCACGAAGTCGCTCGTCCTCCGGTCCAGTTGGTTAGCAGGATTTCTGCGCGT
TTGAAGGTAATGGCCAGTCTTGATATCGCTGAGCGGCGCAAACCGCAGGACGGGCGTATCAAGATGAAGGTTTCGAAAAC
CAAATCCATCGACTTCCGCGTCAATACGCTGCCCACGCTATGGGGCGAAAAAATCGTAATGCGGATCCTGGACTCGTCAA
GCGCCAAGATGGGCATTGATGCGCTGGGCTATGAGGAAGACCAGAAAGAGTTGTATCTGACCGCGCTGAAGCAGCCGCAG
GGGATGATTCTGGTTACCGGCCCGACAGGCTCGGGCAAGACTGTCTCGCTCTATACCGGCCTGAACATCCTCAATACGGT
CGACGTGAACATCTCAACGGCCGAGGATCCGGTGGAGATCAACCTCGAGGGGATCAATCAGGTCAACGTTAACCCAAGGC
AGGGCATGGACTTCGCACAGGCCCTGCGTGCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGTGAGATTCGCGAC
CTGGAAACTGCCGAGATCGCGATCAAGGCGGCACAGACCGGCCACATGGTGATGTCGACGCTGCATACCAATAGTGCGGC
AGAGACCCTGACGCGGTTGCGCAACATGGGAGTGCCGGCTTTCAACATCGCGACCTCGGTGAACTTGATCATTGCTCAGC
GGCTGGCGCGAAAACTTTGCCCGAGCTGCAAGGCGGAAGTTGATGTGCCGCGTGAGGTATTGCTCAAGGAGGGCTTCCCG
GAAGCTCGGATAGGCACCTTCAAGCTCTACGGGCCGGTTGGTTGCGACAGTTGCAAGGGGGGCTACAAGGGGCGTGTCGG
TATTTATGAAGTGGTTAAAATCACGCCCTCGCTGCAGCGACTTATCATGGAAGAAGGAAATTCCATCGATATCGCACTTC
AGGCGCGCAAGGATGGCTTCAACGACCTGCGCACCTCTGGCTTGGTCAAGGCCATGAATGGCGTAACCAGTCTGGAAGAA
GTCAACCGCGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.246

100

0.586
  pilB Acinetobacter baylyi ADP1

56.643

100

0.571
  pilB Legionella pneumophila strain ERS1305867

54.674

100

0.547
  pilB Vibrio cholerae strain A1552

51.381

95.767

0.492
  pilF Neisseria gonorrhoeae MS11

50.645

95.767

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

53.346

89.594

0.478
  pilB Vibrio campbellii strain DS40M4

52.663

89.418

0.471
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.261

100

0.393
  pilF Thermus thermophilus HB27

38.544

99.295

0.383
  pilB/pilB1 Synechocystis sp. PCC 6803

40.462

91.534

0.37

Multiple sequence alignment