Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GFH30_RS12025 Genome accession   NZ_CP045650
Coordinates   2512320..2513549 (+) Length   409 a.a.
NCBI ID   WP_153372898.1    Uniprot ID   A0A5Q0P4F9
Organism   Acinetobacter wanghuae strain dk386     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2507320..2518549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFH30_RS11980 (GFH30_11975) rimP 2507578..2508102 (-) 525 WP_153372890.1 ribosome maturation factor RimP -
  GFH30_RS12010 (GFH30_12005) secG 2509167..2509496 (-) 330 WP_153372892.1 preprotein translocase subunit SecG -
  GFH30_RS12015 (GFH30_12010) tpiA 2509510..2510301 (-) 792 WP_153372894.1 triose-phosphate isomerase -
  GFH30_RS12020 (GFH30_12015) pilB 2510581..2512287 (+) 1707 WP_153372896.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GFH30_RS12025 (GFH30_12020) pilC 2512320..2513549 (+) 1230 WP_153372898.1 type II secretion system F family protein Machinery gene
  GFH30_RS12030 (GFH30_12025) pilD 2513549..2514409 (+) 861 WP_153372899.1 A24 family peptidase Machinery gene
  GFH30_RS12035 (GFH30_12030) coaE 2514411..2515010 (+) 600 WP_153372901.1 dephospho-CoA kinase -
  GFH30_RS12040 (GFH30_12035) - 2515028..2515948 (-) 921 WP_153372903.1 DMT family transporter -
  GFH30_RS12045 (GFH30_12040) rlmB 2515987..2516736 (-) 750 WP_153372905.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GFH30_RS12050 (GFH30_12045) - 2516876..2517199 (-) 324 WP_153372907.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 45090.59 Da        Isoelectric Point: 9.8759

>NTDB_id=395925 GFH30_RS12025 WP_153372898.1 2512320..2513549(+) (pilC) [Acinetobacter wanghuae strain dk386]
MAVAKKGQMMPTFVYEGVDRKGVKLKGELPARNMALAKVTLRKQGISIKTIREKRKNILEGLMKKKVSTLDITVFTRQLA
TMMKAGVPLVQGFEIVAEGLENPSMREVVLGIKGEVEGGNTFAGALRKYPQYFDKLFCSLVESGEQSGSLETMLDRVAIY
KEKSELLKQKIKKAMKYPAAVVVVAIVVTIILMVKVVPVFEELFTSFGADLPAFTKMVVNMSQWTQKYWFLLIIFIALVI
TAFLEAKKRSKKFRDFLDKAALKAPIFGDLVYKAIIARYSRTLATTFAAGVPLIDALESTAGATNNCVYEDAVMKIREDV
ATGQQLQFAMRVTNKFPSMAIQMVAIGEESGALDSMLDKVATHYENEVDNAVDGLTSMMEPLIMAVLGVLVGGLVIAMYL
PIFQMGSVV

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=395925 GFH30_RS12025 WP_153372898.1 2512320..2513549(+) (pilC) [Acinetobacter wanghuae strain dk386]
ATGGCAGTAGCGAAAAAAGGTCAAATGATGCCGACCTTCGTTTATGAAGGGGTTGATCGTAAAGGGGTAAAACTCAAAGG
TGAATTGCCTGCACGTAATATGGCATTGGCAAAAGTCACCTTACGTAAACAGGGTATTAGCATTAAAACCATTCGAGAAA
AACGCAAAAATATTCTTGAAGGTTTAATGAAAAAGAAGGTCTCTACCTTAGATATTACTGTCTTTACTCGTCAGTTAGCG
ACCATGATGAAAGCCGGTGTACCCTTAGTGCAAGGCTTTGAAATTGTAGCTGAAGGCTTAGAAAACCCCTCTATGCGTGA
AGTAGTTTTGGGCATTAAAGGTGAAGTGGAAGGTGGTAATACTTTCGCTGGTGCACTGCGTAAATATCCTCAGTACTTTG
ATAAGCTTTTTTGTTCTTTGGTGGAATCAGGTGAGCAGTCAGGCTCACTCGAAACCATGTTAGATCGTGTTGCTATTTAT
AAAGAAAAGAGCGAGCTATTAAAGCAAAAGATTAAAAAAGCCATGAAATACCCCGCGGCTGTTGTGGTCGTAGCTATCGT
GGTGACGATTATCCTCATGGTGAAAGTGGTTCCCGTATTTGAAGAACTCTTTACATCATTTGGTGCCGACCTACCCGCAT
TTACCAAAATGGTCGTCAACATGTCACAGTGGACACAAAAATATTGGTTCCTACTGATTATCTTTATTGCGCTTGTCATT
ACTGCCTTTTTGGAAGCAAAAAAACGTAGTAAAAAATTCCGTGACTTCTTAGATAAAGCTGCACTTAAAGCACCTATTTT
TGGTGATTTGGTCTATAAAGCGATTATTGCGCGTTATAGCCGTACTTTAGCCACCACATTTGCTGCCGGTGTACCGTTAA
TTGATGCCCTTGAATCGACTGCTGGTGCAACCAATAACTGTGTTTACGAAGATGCGGTGATGAAAATTCGTGAAGATGTA
GCAACCGGTCAACAGTTGCAATTTGCCATGCGTGTGACCAATAAATTCCCCTCAATGGCCATTCAAATGGTCGCGATTGG
TGAAGAATCAGGTGCACTTGACTCTATGCTTGATAAAGTTGCAACCCATTATGAAAATGAAGTCGATAATGCGGTTGATG
GTCTCACCTCGATGATGGAACCTCTTATTATGGCGGTGCTTGGTGTCCTTGTCGGTGGCTTAGTGATTGCAATGTATCTA
CCAATCTTCCAAATGGGCTCTGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(280-401)

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q0P4F9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baumannii D1279779

88.998

100

0.89
  pilC Acinetobacter baylyi ADP1

83.99

99.267

0.834
  pilC Pseudomonas stutzeri DSM 10701

59.951

99.511

0.597
  pilC Legionella pneumophila strain ERS1305867

55.25

97.8

0.54
  pilG Neisseria gonorrhoeae MS11

46.867

97.555

0.457
  pilG Neisseria meningitidis 44/76-A

46.867

97.555

0.457
  pilC Vibrio cholerae strain A1552

42.752

99.511

0.425
  pilC Vibrio campbellii strain DS40M4

42.394

98.044

0.416

Multiple sequence alignment