Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GFH30_RS12020 Genome accession   NZ_CP045650
Coordinates   2510581..2512287 (+) Length   568 a.a.
NCBI ID   WP_153372896.1    Uniprot ID   A0A5Q0P5M6
Organism   Acinetobacter wanghuae strain dk386     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2505581..2517287
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFH30_RS11975 (GFH30_11970) nusA 2506058..2507542 (-) 1485 WP_153372888.1 transcription termination factor NusA -
  GFH30_RS11980 (GFH30_11975) rimP 2507578..2508102 (-) 525 WP_153372890.1 ribosome maturation factor RimP -
  GFH30_RS12010 (GFH30_12005) secG 2509167..2509496 (-) 330 WP_153372892.1 preprotein translocase subunit SecG -
  GFH30_RS12015 (GFH30_12010) tpiA 2509510..2510301 (-) 792 WP_153372894.1 triose-phosphate isomerase -
  GFH30_RS12020 (GFH30_12015) pilB 2510581..2512287 (+) 1707 WP_153372896.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GFH30_RS12025 (GFH30_12020) pilC 2512320..2513549 (+) 1230 WP_153372898.1 type II secretion system F family protein Machinery gene
  GFH30_RS12030 (GFH30_12025) pilD 2513549..2514409 (+) 861 WP_153372899.1 A24 family peptidase Machinery gene
  GFH30_RS12035 (GFH30_12030) coaE 2514411..2515010 (+) 600 WP_153372901.1 dephospho-CoA kinase -
  GFH30_RS12040 (GFH30_12035) - 2515028..2515948 (-) 921 WP_153372903.1 DMT family transporter -
  GFH30_RS12045 (GFH30_12040) rlmB 2515987..2516736 (-) 750 WP_153372905.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  GFH30_RS12050 (GFH30_12045) - 2516876..2517199 (-) 324 WP_153372907.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63498.78 Da        Isoelectric Point: 4.9777

>NTDB_id=395924 GFH30_RS12020 WP_153372896.1 2510581..2512287(+) (pilB) [Acinetobacter wanghuae strain dk386]
MTALQGSIRFTGFIRRIVDEGLLSAEHMQAALINSKRDKVDIVPYLIEHHKISAAIIAEKISTEFGEPLFDISAYDPAQI
LRDEIDEKLITKHRMLPIFKNSSILYIATSNPTNVEAVDAVRFATKLNIETIIVEHNKLEKLIEQNFAEESTFDFDDDFD
LDVDVDTSDPNKEEDTDKGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRVDGVLRQIATPPLQLANRLSSRL
KVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKELFMQALDKPQG
MLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSVALKSFLRQDPDIVMVGEIRDL
ETAEIAVKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPKQSLLEMGFTE
EDLQNPEFQVYEPIGCSECREGYKGRVGVYEVMKVTPEISRIIMEDGNAIEIAEASARAGFNNLRRSGLLKVMQGVTSLQ
EVNRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=395924 GFH30_RS12020 WP_153372896.1 2510581..2512287(+) (pilB) [Acinetobacter wanghuae strain dk386]
ATGACAGCACTTCAGGGATCTATCCGATTTACAGGTTTTATTCGTCGTATTGTCGATGAGGGTCTTTTGTCTGCCGAACA
TATGCAGGCCGCTTTAATTAACTCCAAACGCGATAAAGTCGATATCGTGCCGTACTTGATTGAGCACCATAAAATTTCAG
CAGCAATCATTGCAGAAAAAATTTCGACTGAATTTGGTGAACCTTTATTTGATATTAGCGCCTATGATCCGGCACAAATT
CTACGCGATGAAATTGATGAAAAACTCATTACCAAGCATCGCATGCTGCCAATCTTTAAAAATTCCAGCATTTTATATAT
TGCCACCAGTAATCCGACCAATGTTGAAGCTGTGGATGCCGTTCGTTTCGCCACAAAACTCAATATTGAAACCATTATTG
TTGAACACAACAAACTTGAAAAACTAATAGAACAAAACTTTGCTGAAGAAAGCACTTTCGATTTTGATGACGATTTTGAC
CTTGATGTTGATGTCGACACTTCTGATCCAAACAAAGAAGAAGATACAGACAAAGGCGATGAAGCTCCTATTGTAAAATA
CATCAATAAGTTATTGATCGATGCCATCCGCATGGGCGCATCAGATTTACACTTTGAGCCTTATGAAAAGATTTATCGTG
TCCGTTATCGTGTCGATGGTGTATTGCGTCAAATTGCTACTCCGCCACTACAATTAGCCAATCGTCTTTCATCACGCTTA
AAAGTCATGTCACAAATGGATATTTCTGAAAAACGTGTGCCTCAAGACGGTCGTATTAAATTAAAACTTTCCAAAAATAA
AGCGATTGATTTCCGTGTGAACTCATTACCAACGCTCTTTGGTGAAAAGTTAGTATTACGTATTTTGGATCCATCCAGTG
CGATGCTCGGCATTGATGCCTTGGGTTATGAACCTGATCAAAAAGAACTGTTTATGCAGGCTTTGGATAAACCGCAAGGA
ATGCTACTCATTACGGGTCCGACGGGTTCTGGTAAAACGGTTTCTTTATATACGGGCTTAAATATTCTCAATCGTGAAGA
TACCAATATTTCTACTGCCGAAGATCCAGTGGAAATTAACTTACAAGGCATTAATCAGGTTAACGTTAATAATAAAGTTG
GTTTAACTTTCTCTGTCGCATTGAAGTCTTTCTTACGTCAAGATCCAGATATTGTCATGGTCGGTGAGATTCGAGACTTA
GAAACAGCCGAAATCGCAGTTAAAGCGGCACAAACTGGACATATGGTGATGTCGACACTGCATACCAATAGTGCGCCAGA
AACCTTAACGCGTTTACGTAATATGGGTGTGCCCTCTTTTAATATCGCGACCTCGGTCAATCTTGTGATCGCACAACGAC
TCGCACGTCGTTTATGCTCGCAATGCAAAAAACCAGCAGATATTCCAAAACAAAGTTTATTGGAAATGGGCTTTACCGAA
GAAGACCTCCAAAATCCAGAGTTTCAGGTCTATGAACCCATTGGCTGTAGTGAATGCCGAGAAGGTTATAAAGGTCGTGT
CGGTGTGTATGAAGTCATGAAAGTTACACCAGAAATTTCAAGAATTATTATGGAAGATGGCAATGCAATTGAGATTGCCG
AAGCTTCCGCTCGTGCAGGTTTTAACAATTTACGCCGATCAGGTTTATTAAAGGTTATGCAGGGGGTGACTTCTTTACAG
GAAGTCAATCGTGTGACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q0P5M6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.298

100

0.796
  pilB Acinetobacter baylyi ADP1

75.088

100

0.754
  pilB Legionella pneumophila strain ERS1305867

52.886

94.542

0.5
  pilB Vibrio cholerae strain A1552

49.367

97.359

0.481
  pilF Neisseria gonorrhoeae MS11

48.036

98.592

0.474
  pilB Vibrio parahaemolyticus RIMD 2210633

48.288

97.711

0.472
  pilB Vibrio campbellii strain DS40M4

48.014

97.535

0.468
  pilF Thermus thermophilus HB27

37.117

97.711

0.363
  pilB/pilB1 Synechocystis sp. PCC 6803

33.605

100

0.363

Multiple sequence alignment