Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GFH30_RS11795 Genome accession   NZ_CP045650
Coordinates   2464574..2466682 (-) Length   702 a.a.
NCBI ID   WP_153372825.1    Uniprot ID   A0A5Q0P8P8
Organism   Acinetobacter wanghuae strain dk386     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2459574..2471682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFH30_RS11780 (GFH30_11775) - 2462029..2462880 (-) 852 WP_153372819.1 hypothetical protein -
  GFH30_RS11785 (GFH30_11780) aroB 2462892..2463971 (-) 1080 WP_153372821.1 3-dehydroquinate synthase -
  GFH30_RS11790 (GFH30_11785) aroK 2464001..2464537 (-) 537 WP_153372823.1 shikimate kinase AroK -
  GFH30_RS11795 (GFH30_11790) pilQ 2464574..2466682 (-) 2109 WP_153372825.1 type IV pilus secretin PilQ Machinery gene
  GFH30_RS11800 (GFH30_11795) pilP 2466700..2467227 (-) 528 WP_153372827.1 pilus assembly protein PilP Machinery gene
  GFH30_RS11805 (GFH30_11800) comO 2467227..2467934 (-) 708 WP_153389405.1 type 4a pilus biogenesis protein PilO Machinery gene
  GFH30_RS11810 (GFH30_11805) comN 2467931..2468575 (-) 645 WP_153372831.1 PilN domain-containing protein Machinery gene
  GFH30_RS11815 (GFH30_11810) pilM 2468575..2469633 (-) 1059 WP_153372833.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 702 a.a.        Molecular weight: 76259.29 Da        Isoelectric Point: 5.6826

>NTDB_id=395919 GFH30_RS11795 WP_153372825.1 2464574..2466682(-) (pilQ) [Acinetobacter wanghuae strain dk386]
MNKSIRKFNFGDGQTMNHVFRQFSMAAITIAITQVASAQVSITNVVPLQIPGQGTEIRVMFNGLPPQPQAYQLESPSRLI
LDFDQVQQNVKQTTVNVATAEASAVDLSSDAQRARLTVNLADAGAFTTRVEGNTFILKINSAKANVVAPSVTQQAVSGVS
NIGFQRGSKGEGQVVIDLAGSNTPVDVQQQGTKVIVRTLGSKIPTHLIRRLSVNDFATPASTIDSINDNGSGLITIQTSG
SYEYMAYQTDNKLTISLKRPEDKNPLRPKVNNHYSGKKISLDFQDIEVRRVLQLLADFTDINMVAADSVQGNITLRLKEV
PWDQALDIVLKTKKLDKRRNGNVIWIAPIEELTKAEADEAKAIAQSIKLSPLQTEYIQLSYAKAADIEKLLTSSKKATNS
SSSSGGDNGDDPLGEANLLSPRGTVSVDTRTNTLIINDTSQRIDQIRKMIDLLDVAVKQVMVEARIVTAERTFSRDLGIK
WGLQRNNPTFTGGPNGSNVWGGDFSPRVDLGVDLSTASAGTFNFGLLKISDYMLDLQLTALQSDGLGEVLSAPKILTGDK
QKAYILRGTEIPYQTYSANEGSTTSFKEANLKLEVTPSITPEGKVQLELKVEKDTIGEMTSEGPSINTNELQTNVLINDG
ETVVLGGIFDDFKTTTSDKVPFLGDLPVVGNLFKNNSKISNQAELLIFVTPRIVNDTVSRNH

Nucleotide


Download         Length: 2109 bp        

>NTDB_id=395919 GFH30_RS11795 WP_153372825.1 2464574..2466682(-) (pilQ) [Acinetobacter wanghuae strain dk386]
ATGAATAAAAGTATCAGAAAATTTAATTTTGGGGATGGTCAGACAATGAATCATGTGTTTCGTCAGTTTTCAATGGCTGC
AATTACAATAGCAATTACCCAGGTGGCGAGTGCGCAGGTTTCAATAACCAATGTCGTACCTTTACAAATTCCAGGTCAAG
GCACAGAAATTCGTGTCATGTTCAATGGTTTACCACCACAGCCTCAAGCCTACCAATTAGAATCACCGTCACGTTTAATT
CTTGATTTTGATCAAGTGCAACAAAATGTGAAACAAACTACGGTCAATGTTGCTACAGCAGAAGCCTCAGCGGTTGATCT
AAGCTCAGATGCACAACGCGCGCGCCTTACCGTAAACTTAGCAGATGCCGGTGCATTTACGACACGTGTTGAAGGCAACA
CCTTTATCCTGAAAATTAACTCTGCTAAAGCAAATGTAGTAGCACCGTCTGTTACGCAACAAGCCGTATCAGGTGTATCG
AACATTGGTTTCCAACGTGGTTCAAAAGGTGAAGGGCAAGTTGTAATTGACTTGGCTGGATCAAATACCCCTGTGGATGT
TCAGCAACAAGGAACAAAAGTGATTGTAAGAACATTAGGTTCTAAAATTCCAACACATCTTATCCGTCGCTTGAGCGTAA
ATGATTTCGCCACTCCAGCATCGACCATTGATTCAATTAATGATAATGGGTCTGGTTTAATTACGATTCAAACTTCTGGT
AGCTATGAATACATGGCATACCAGACGGACAATAAGCTGACAATTAGCTTAAAACGCCCAGAAGATAAGAATCCATTGAG
ACCGAAAGTAAATAATCATTATAGTGGCAAAAAGATTTCTCTTGATTTTCAGGATATTGAAGTACGACGTGTTTTACAGC
TTTTAGCTGATTTTACGGATATCAATATGGTTGCTGCAGACTCCGTGCAAGGAAATATTACCCTGCGATTAAAAGAAGTA
CCGTGGGATCAAGCACTTGATATTGTACTGAAGACAAAAAAATTAGATAAACGTCGTAATGGTAATGTCATTTGGATCGC
ACCAATAGAAGAATTGACTAAAGCGGAAGCGGATGAAGCTAAGGCGATAGCTCAGTCGATTAAACTTTCACCTTTACAAA
CTGAATATATTCAACTTAGTTATGCTAAAGCAGCGGACATTGAAAAATTGCTGACATCATCTAAAAAAGCAACTAATTCA
TCTTCTTCATCAGGTGGTGATAATGGCGATGATCCTTTAGGTGAAGCAAATCTACTCAGCCCACGTGGTACAGTTTCAGT
AGATACGCGAACCAATACTTTAATTATTAATGATACATCACAACGCATTGATCAAATACGTAAGATGATCGATCTTTTAG
ATGTTGCAGTCAAACAAGTGATGGTTGAAGCACGTATTGTAACTGCAGAAAGAACCTTCTCTCGTGATTTAGGGATTAAA
TGGGGCTTACAACGTAATAATCCAACGTTTACAGGTGGGCCAAATGGTTCTAATGTGTGGGGTGGTGATTTCTCACCACG
TGTTGATCTAGGTGTAGATCTGAGTACAGCCTCTGCAGGTACGTTTAACTTTGGCTTATTGAAGATTTCTGATTATATGC
TCGATCTTCAATTGACAGCCTTACAGTCGGATGGTCTGGGTGAAGTGTTATCAGCGCCTAAAATTTTAACAGGGGATAAG
CAAAAAGCTTATATTTTACGTGGAACAGAAATTCCTTATCAAACTTATAGTGCCAATGAGGGCTCCACTACCTCATTTAA
AGAAGCTAATTTGAAGTTAGAAGTGACACCAAGCATTACACCTGAAGGAAAAGTTCAACTTGAACTTAAAGTTGAGAAAG
ATACCATCGGTGAGATGACATCTGAAGGACCATCAATTAATACCAATGAATTACAGACCAATGTACTTATTAATGATGGG
GAGACAGTGGTGCTCGGAGGTATTTTCGATGACTTTAAAACGACAACTTCGGATAAAGTTCCATTCTTAGGTGATTTACC
GGTTGTGGGTAATTTATTCAAGAATAATTCGAAAATTTCTAACCAAGCAGAGTTGCTGATTTTTGTTACACCACGAATTG
TTAATGACACTGTTTCAAGAAATCATTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(375-459)

AMIN domain (AMIN)

(55-135)

Bacterial type II and III secretion system protein (Secretin)

(540-694)

Secretin and TonB N terminus short domain (STN)

(306-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Q0P8P8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Acinetobacter baumannii D1279779

63.347

100

0.652
  pilQ Acinetobacter baumannii strain A118

63.209

100

0.651
  comQ Acinetobacter baylyi ADP1

61.657

100

0.625
  pilQ Pseudomonas aeruginosa PAK

40.49

98.86

0.4
  pilQ Legionella pneumophila strain ERS1305867

37.281

97.436

0.363
  pilQ Legionella pneumophila strain Lp02

37.135

97.436

0.362

Multiple sequence alignment