Detailed information NTG0395923

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: GFH30_RS11815
• Genome accession: NZ_CP045650
• Coordinates: 2468575..2469633 (-)
• Length: 352 a.a.
• NCBI ID: WP_153372833.1
• Uniprot ID: -
• Organism: Acinetobacter wanghuae strain dk386
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2463575..2474633

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GFH30_RS11790 (GFH30_11785)	aroK	2464001..2464537 (-)	537	WP_153372823.1	shikimate kinase AroK	-
GFH30_RS11795 (GFH30_11790)	pilQ	2464574..2466682 (-)	2109	WP_153372825.1	type IV pilus secretin PilQ	Machinery gene
GFH30_RS11800 (GFH30_11795)	pilP	2466700..2467227 (-)	528	WP_153372827.1	pilus assembly protein PilP	Machinery gene
GFH30_RS11805 (GFH30_11800)	comO	2467227..2467934 (-)	708	WP_153389405.1	type 4a pilus biogenesis protein PilO	Machinery gene
GFH30_RS11810 (GFH30_11805)	comN	2467931..2468575 (-)	645	WP_153372831.1	PilN domain-containing protein	Machinery gene
GFH30_RS11815 (GFH30_11810)	pilM	2468575..2469633 (-)	1059	WP_153372833.1	pilus assembly protein PilM	Machinery gene
GFH30_RS11820 (GFH30_11815)	ponA	2469800..2472325 (+)	2526	WP_153372835.1	penicillin-binding protein PBP1a	-
GFH30_RS11825 (GFH30_11820)	-	2472377..2473180 (-)	804	WP_153372837.1	putative RNA methyltransferase	-
GFH30_RS11830 (GFH30_11825)	mraY	2473239..2474357 (-)	1119	WP_153372839.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38931.43 Da
• Isoelectric Point: 4.6810

>NTDB_id=395923 GFH30_RS11815 WP_153372833.1 2468575..2469633(-) (pilM) [Acinetobacter wanghuae strain dk386]
MRRLYRKPNKGLIGVDISSTSVKVLELSMKSGRYWVESYALIPLPEGSVVEKNILNPEAVGDALGRAINLANTQSNQAAF
AIPTSMVITKTIEMDADMSSDEREVQIRDDAEQYIPFPLDEASLDFEVLPDRLSNPNRVNVLLVATRIENVEARSEVLQI
AGTHPKIADVESFALENAFKVFSDTLPMGVNTVGILDIGHSMTTLSVMQNNKIIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKTRALPDDYDIEVLEPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTVANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=395923 GFH30_RS11815 WP_153372833.1 2468575..2469633(-) (pilM) [Acinetobacter wanghuae strain dk386]
GTGCGCAGGTTATATCGTAAGCCAAATAAGGGGTTAATAGGCGTCGACATTAGTTCGACCTCTGTTAAAGTTTTAGAACT
TTCTATGAAAAGCGGGCGGTATTGGGTAGAGAGCTATGCTCTCATCCCGCTACCAGAAGGAAGTGTCGTTGAAAAAAACA
TCTTAAACCCAGAAGCGGTTGGAGATGCTTTAGGTCGTGCCATTAATTTGGCAAACACACAGTCGAATCAGGCAGCGTTT
GCAATTCCGACCTCAATGGTGATTACCAAAACCATTGAAATGGATGCAGATATGTCGAGCGATGAGCGTGAAGTTCAAAT
TCGCGATGACGCTGAACAATATATTCCATTCCCACTCGATGAAGCGAGCCTAGATTTTGAGGTTCTGCCTGATCGATTGT
CCAATCCCAATCGCGTCAATGTATTATTGGTCGCAACCCGAATTGAAAACGTAGAAGCACGTTCAGAAGTATTGCAAATT
GCTGGTACACATCCAAAAATTGCCGATGTAGAAAGCTTTGCGCTTGAAAATGCTTTTAAAGTGTTTTCCGATACGTTGCC
AATGGGTGTCAATACGGTCGGTATTTTAGATATCGGGCACAGTATGACCACCTTGTCGGTCATGCAAAACAATAAAATTA
TTTATACCCGAGAGCAAGTCTTTGGGGGTAAACAACTGACCCAAGAAATTCAAAACCGTTATGGATTGTCATTTGAAGAA
GCAGGACGTGCCAAGAAAACACGTGCCTTGCCAGATGATTATGACATTGAAGTACTTGAGCCATTTTTGGATGCTGTGGT
GCAGCAAGCCGCACGTTCATTACAGTTCTTCTTTTCATCATCTCAGTTTAACGAGATTGATCATATTTTATTGGCTGGGG
GCAATGCCAATATTCCAGGCCTCGCAAAATTATTACAGCAAAAACTCGGTTACCGTGTCACCGTCGCGAATCCATTTTTA
CAAATGGGCTTTTCTCCTCAAATCGATATTAAAAAAATTGAAAATGACGCGTCATCACTCATGGTTGCATGCGGTTTAGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	84.375	100	0.844
comM	Acinetobacter nosocomialis M2	84.375	100	0.844
comM	Acinetobacter baylyi ADP1	77.557	100	0.776
pilM	Legionella pneumophila strain ERS1305867	39.548	100	0.398