Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   LF543_RS02565 Genome accession   NZ_CP045562
Coordinates   502144..502566 (+) Length   140 a.a.
NCBI ID   WP_010021672.1    Uniprot ID   A0AAE6NZT2
Organism   Fructilactobacillus fructivorans strain LF543     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 489190..527840 502144..502566 within 0


Gene organization within MGE regions


Location: 489190..527840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LF543_RS02455 (LF543_02425) - 489190..489822 (-) 633 WP_010021710.1 deoxynucleoside kinase -
  LF543_RS02460 (LF543_02430) - 490010..490666 (+) 657 WP_225429585.1 type II CAAX endopeptidase family protein -
  LF543_RS02465 (LF543_02435) - 490678..491307 (+) 630 WP_010021707.1 miniconductance mechanosensitive channel MscM -
  LF543_RS02470 (LF543_02440) - 491307..491807 (+) 501 WP_010021706.1 phosphatidylglycerophosphatase A -
  LF543_RS02475 (LF543_02445) - 491842..492069 (-) 228 WP_010021705.1 hypothetical protein -
  LF543_RS02480 - 493189..493350 (+) 162 WP_010021703.1 hypothetical protein -
  LF543_RS02485 (LF543_02450) - 493639..494460 (+) 822 WP_010021701.1 glycosyltransferase family 8 protein -
  LF543_RS02490 (LF543_02455) - 494851..495972 (-) 1122 WP_010021700.1 site-specific integrase -
  LF543_RS02495 (LF543_02460) - 496113..496487 (-) 375 WP_010021699.1 hypothetical protein -
  LF543_RS02500 (LF543_02465) - 496547..496900 (-) 354 WP_010021698.1 ImmA/IrrE family metallo-endopeptidase -
  LF543_RS02505 (LF543_02470) - 497166..497486 (-) 321 WP_010021696.1 hypothetical protein -
  LF543_RS02510 (LF543_02475) - 497523..497861 (-) 339 WP_056936270.1 helix-turn-helix domain-containing protein -
  LF543_RS02515 (LF543_02480) - 498110..498319 (+) 210 WP_010021688.1 hypothetical protein -
  LF543_RS02520 - 498325..498465 (+) 141 WP_010021687.1 hypothetical protein -
  LF543_RS02525 (LF543_02485) - 498871..499125 (+) 255 WP_010021683.1 hypothetical protein -
  LF543_RS02530 (LF543_02490) - 499106..499345 (-) 240 WP_029327108.1 hypothetical protein -
  LF543_RS02535 (LF543_02495) - 499409..499621 (+) 213 WP_010021681.1 MW1434 family type I TA system toxin -
  LF543_RS02540 (LF543_02500) - 499675..499881 (+) 207 WP_010021680.1 hypothetical protein -
  LF543_RS02545 (LF543_02505) - 499856..500377 (-) 522 WP_010021679.1 hypothetical protein -
  LF543_RS02550 (LF543_02510) - 500433..500690 (+) 258 WP_010021677.1 helix-turn-helix domain-containing protein -
  LF543_RS02555 (LF543_02515) - 500925..501404 (+) 480 WP_010021674.1 siphovirus Gp157 family protein -
  LF543_RS02560 (LF543_02520) - 501413..502147 (+) 735 WP_010021673.1 DUF1071 domain-containing protein -
  LF543_RS02565 (LF543_02525) ssb 502144..502566 (+) 423 WP_010021672.1 single-stranded DNA-binding protein Machinery gene
  LF543_RS02570 (LF543_02530) - 502587..503402 (+) 816 WP_010021671.1 hypothetical protein -
  LF543_RS02575 (LF543_02535) - 503437..504147 (+) 711 WP_201781237.1 phage regulatory protein/antirepressor Ant -
  LF543_RS02580 (LF543_02540) - 504140..504538 (+) 399 WP_010021668.1 DUF1064 domain-containing protein -
  LF543_RS02585 (LF543_02545) - 504551..504973 (+) 423 WP_010021667.1 hypothetical protein -
  LF543_RS02590 (LF543_02550) - 505299..505721 (+) 423 WP_010021666.1 autolysin regulatory protein arpU -
  LF543_RS02595 (LF543_02555) - 506212..506727 (+) 516 WP_010021665.1 HNH endonuclease -
  LF543_RS02600 (LF543_02560) - 507004..507507 (+) 504 WP_081454149.1 phage terminase small subunit P27 family -
  LF543_RS02605 (LF543_02565) - 507504..509390 (+) 1887 WP_010021662.1 terminase large subunit -
  LF543_RS02610 (LF543_02570) - 509406..509603 (+) 198 WP_010021660.1 DUF1056 family protein -
  LF543_RS02615 (LF543_02575) - 509603..510712 (+) 1110 WP_010021659.1 phage portal protein -
  LF543_RS02620 (LF543_02580) - 510732..511451 (+) 720 WP_010021658.1 head maturation protease, ClpP-related -
  LF543_RS02625 (LF543_02585) - 511469..512695 (+) 1227 WP_010021657.1 phage major capsid protein -
  LF543_RS02630 (LF543_02590) - 512701..513030 (+) 330 WP_010021655.1 head-tail connector protein -
  LF543_RS02635 (LF543_02595) - 513023..513367 (+) 345 WP_010021653.1 phage head closure protein -
  LF543_RS02640 (LF543_02600) - 513357..513776 (+) 420 WP_010021652.1 HK97-gp10 family putative phage morphogenesis protein -
  LF543_RS02645 (LF543_02605) - 513769..514140 (+) 372 WP_056936271.1 DUF806 family protein -
  LF543_RS02650 (LF543_02610) - 514156..514749 (+) 594 WP_010021650.1 major tail protein -
  LF543_RS02655 (LF543_02615) - 514889..515305 (+) 417 WP_010021649.1 phage tail tube assembly chaperone -
  LF543_RS02660 - 515362..515520 (+) 159 WP_010021648.1 hypothetical protein -
  LF543_RS02665 (LF543_02620) - 515537..520321 (+) 4785 WP_010021647.1 tape measure protein -
  LF543_RS02670 (LF543_02625) - 520321..521160 (+) 840 WP_010021644.1 phage tail domain-containing protein -
  LF543_RS02675 (LF543_02630) - 521172..522401 (+) 1230 WP_010021643.1 hypothetical protein -
  LF543_RS02680 (LF543_02635) - 522401..524188 (+) 1788 WP_010021641.1 phage tail protein -
  LF543_RS02685 (LF543_02640) - 524201..524740 (+) 540 WP_010021639.1 hypothetical protein -
  LF543_RS02690 (LF543_02645) - 524740..525255 (+) 516 WP_010021638.1 hypothetical protein -
  LF543_RS02695 (LF543_02650) - 525270..525773 (+) 504 WP_010021636.1 hypothetical protein -
  LF543_RS02700 (LF543_02655) - 525790..526185 (+) 396 WP_010021635.1 hypothetical protein -
  LF543_RS02705 (LF543_02660) - 526188..526334 (+) 147 WP_010021634.1 XkdX family protein -
  LF543_RS07040 - 526381..526668 (+) 288 WP_010021633.1 hypothetical protein -
  LF543_RS02715 (LF543_02670) - 526649..526921 (+) 273 WP_010021632.1 hypothetical protein -
  LF543_RS02720 (LF543_02675) - 526905..527840 (+) 936 WP_010021630.1 N-acetylmuramoyl-L-alanine amidase -

Sequence


Protein


Download         Length: 140 a.a.        Molecular weight: 15606.09 Da        Isoelectric Point: 4.8538

>NTDB_id=395164 LF543_RS02565 WP_010021672.1 502144..502566(+) (ssb) [Fructilactobacillus fructivorans strain LF543]
MINTTVFTGRVTKALEVRQTSNGKSVGSVNLAVNRNFKNQDGNYDADFPMLQFWGKMADNFAKYTSKGSLVGIEGRLQTR
NYEDKNGNRVYVTEIVVNNFTLLDSKNNNNQSNNSNPFENPSAPTNNTGEPEISDDDLPF

Nucleotide


Download         Length: 423 bp        

>NTDB_id=395164 LF543_RS02565 WP_010021672.1 502144..502566(+) (ssb) [Fructilactobacillus fructivorans strain LF543]
ATGATTAATACTACGGTTTTTACAGGAAGAGTTACTAAAGCTCTAGAAGTTAGACAAACTAGCAATGGGAAAAGCGTTGG
CAGTGTAAATTTAGCCGTTAATCGTAATTTTAAAAATCAAGACGGCAATTACGATGCTGACTTTCCGATGTTGCAATTCT
GGGGAAAGATGGCAGATAACTTTGCCAAATATACGAGTAAAGGTTCATTAGTCGGAATTGAAGGTAGACTACAAACCCGC
AATTATGAAGATAAAAATGGTAATCGAGTTTATGTTACTGAGATTGTGGTTAACAATTTCACGTTGCTAGATTCCAAAAA
TAATAACAACCAATCTAATAACAGTAATCCATTTGAAAATCCAAGTGCACCTACTAATAATACTGGTGAACCTGAGATTT
CTGATGATGATCTTCCGTTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

45.882

100

0.557

  ssbA Bacillus subtilis subsp. subtilis str. 168

39.655

100

0.493

  ssbB Streptococcus sobrinus strain NIDR 6715-7

39.286

100

0.393

  ssbA Streptococcus mutans UA159

37.857

100

0.379

  ssbB/cilA Streptococcus mitis NCTC 12261

37.143

100

0.371

  ssbB/cilA Streptococcus pneumoniae TIGR4

36.429

100

0.364

  ssbB/cilA Streptococcus pneumoniae Rx1

36.429

100

0.364

  ssbB/cilA Streptococcus pneumoniae D39

36.429

100

0.364

  ssbB/cilA Streptococcus pneumoniae R6

36.429

100

0.364


Multiple sequence alignment