Detailed information NTG0394963

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: GAY96_RS00865
• Genome accession: NZ_CP045550
• Coordinates: 180962..182077 (+)
• Length: 371 a.a.
• NCBI ID: WP_228345625.1
• Uniprot ID: A0A9E8FJT4
• Organism: Venatoribacter cucullus strain ASxS5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 175962..187077

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GAY96_RS00840 (GAY96_00835)	-	177154..177417 (-)	264	WP_228345620.1	DUF167 family protein	-
GAY96_RS00845 (GAY96_00840)	-	177444..178037 (-)	594	WP_265573973.1	YggT family protein	-
GAY96_RS00850 (GAY96_00845)	proC	178133..178951 (-)	819	WP_228345622.1	pyrroline-5-carboxylate reductase	-
GAY96_RS00855 (GAY96_00850)	-	178994..179698 (-)	705	WP_265573974.1	YggS family pyridoxal phosphate-dependent enzyme	-
GAY96_RS00860 (GAY96_00855)	pilT	179915..180949 (+)	1035	WP_228345624.1	type IV pilus twitching motility protein PilT	Machinery gene
GAY96_RS00865 (GAY96_00860)	pilU	180962..182077 (+)	1116	WP_228345625.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
GAY96_RS00870 (GAY96_00865)	-	182171..184447 (+)	2277	WP_265573975.1	bifunctional diguanylate cyclase/phosphodiesterase	-
GAY96_RS00875 (GAY96_00870)	ideA	184517..185200 (+)	684	WP_265573976.1	endonuclease	Regulator
GAY96_RS00880 (GAY96_00875)	-	185404..186471 (+)	1068	WP_265573977.1	HlyD family secretion protein	-

Sequence

Protein

• Length: 371 a.a.
• Molecular weight: 41230.40 Da
• Isoelectric Point: 6.2871

>NTDB_id=394963 GAY96_RS00865 WP_228345625.1 180962..182077(+) (pilU) [Venatoribacter cucullus strain ASxS5]
MAIDKFLRLMVEKGASDMFITTGVAPSMKVNGKIMPLTKNALSPEQVREVVFGLMNSKQQDEFEEKKELNFAINASGIGR
FRASAFYQRNTCGMVLRRIETRIPGIDQLGLPDVIKEISMAKRGLILFVGGTGTGKSTSLAAMIGHRNKNSKGHILSIED
PIEFIHSHDGCIVTQREVGLDTESFDVALKNCLRQAPDVIMIGEVRTQETMEQAITFAETGHLCLATLHANNANQALERV
IHFFPPERHNQIWMDLSLNLRAIVAQQLIPTPDGRSRRAVVEVLLNTPLVADLIRKGEVHELKPLMAKSSELGMQTFDQA
LYALYEQGEITYEDAIAHADSPNDLRLMIKLGSETDSDHLDGATRNLRLQD

Nucleotide

• Length: 1116 bp

>NTDB_id=394963 GAY96_RS00865 WP_228345625.1 180962..182077(+) (pilU) [Venatoribacter cucullus strain ASxS5]
ATGGCAATTGATAAGTTTCTGCGTCTGATGGTGGAAAAGGGCGCCTCCGATATGTTCATTACTACCGGTGTGGCACCGTC
GATGAAGGTGAACGGTAAGATTATGCCGCTGACCAAAAATGCGCTGTCGCCCGAGCAGGTGCGGGAGGTGGTGTTCGGTT
TAATGAACAGCAAACAGCAGGATGAGTTTGAAGAAAAAAAGGAACTCAACTTCGCCATTAACGCCTCTGGTATCGGCCGT
TTCCGTGCCAGTGCGTTTTATCAGCGCAATACCTGTGGCATGGTGCTGCGGCGGATTGAAACCCGCATTCCCGGCATTGA
TCAGCTTGGCCTGCCCGATGTTATTAAAGAAATTTCCATGGCCAAGCGCGGCCTGATTTTATTCGTTGGTGGTACCGGTA
CCGGTAAATCCACCTCGCTGGCGGCCATGATCGGCCACCGCAATAAAAACAGTAAGGGCCATATTTTAAGCATCGAAGAC
CCGATTGAATTTATTCACTCGCACGATGGCTGCATTGTCACCCAGCGCGAAGTGGGGCTGGACACCGAAAGCTTTGATGT
GGCGCTGAAAAACTGCCTGCGTCAGGCGCCGGATGTGATCATGATCGGTGAGGTGCGGACTCAGGAAACCATGGAGCAGG
CCATTACCTTTGCGGAAACCGGCCACCTCTGTCTGGCCACCCTGCACGCCAACAACGCTAACCAGGCGCTGGAGCGGGTA
ATCCACTTCTTCCCGCCGGAGCGACACAACCAGATCTGGATGGATCTGTCGCTGAACCTGCGTGCCATTGTGGCGCAGCA
GTTAATTCCGACTCCGGATGGCCGTTCGCGCCGCGCGGTGGTGGAAGTGCTGCTGAATACACCGCTGGTCGCCGACCTGA
TCCGCAAGGGTGAAGTACATGAGCTGAAACCTTTGATGGCGAAATCCAGTGAGCTGGGTATGCAAACCTTCGACCAGGCC
CTGTATGCCCTGTACGAGCAGGGGGAAATTACCTACGAAGATGCCATCGCCCACGCCGATTCACCCAACGATCTGCGCCT
GATGATCAAACTCGGTTCGGAAACCGACTCGGACCATCTGGACGGCGCCACCCGCAATCTGCGCCTGCAGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (12-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	74.324	99.73	0.741
pilU	Acinetobacter baylyi ADP1	61.905	96.226	0.596
pilU	Vibrio cholerae strain A1552	54.472	99.461	0.542
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.023	92.453	0.407
pilT	Pseudomonas aeruginosa PAK	38.841	92.992	0.361
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.12	90.027	0.361
pilT	Vibrio cholerae strain A1552	40.12	90.027	0.361