Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   FIU84_RS04960 Genome accession   NZ_CP045416
Coordinates   1043948..1045168 (+) Length   406 a.a.
NCBI ID   WP_063544459.1    Uniprot ID   -
Organism   Stutzerimonas frequens strain THAF7b     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1038948..1050168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU84_RS04935 (FIU84_04950) - 1039179..1040018 (-) 840 WP_172974159.1 hypothetical protein -
  FIU84_RS04940 (FIU84_04955) - 1040020..1040829 (-) 810 WP_256365787.1 hypothetical protein -
  FIU84_RS04945 - 1041142..1041606 (-) 465 WP_152480900.1 hypothetical protein -
  FIU84_RS21720 (FIU84_04965) - 1041633..1041818 (-) 186 WP_152480901.1 type IV pilin protein -
  FIU84_RS04955 (FIU84_04970) pilB 1042242..1043945 (+) 1704 WP_152480902.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FIU84_RS04960 (FIU84_04975) pilC 1043948..1045168 (+) 1221 WP_063544459.1 type II secretion system F family protein Machinery gene
  FIU84_RS04965 (FIU84_04980) pilD 1045172..1046038 (+) 867 WP_152480903.1 A24 family peptidase Machinery gene
  FIU84_RS04970 (FIU84_04985) coaE 1046170..1046778 (+) 609 WP_152480904.1 dephospho-CoA kinase -
  FIU84_RS04975 (FIU84_04990) yacG 1046775..1046972 (+) 198 WP_102840588.1 DNA gyrase inhibitor YacG -
  FIU84_RS04980 (FIU84_04995) - 1046995..1047675 (-) 681 WP_102840589.1 energy-coupling factor ABC transporter permease -
  FIU84_RS04985 (FIU84_05005) - 1047714..1048750 (-) 1037 Protein_977 NADP(H)-dependent aldo-keto reductase -
  FIU84_RS04990 (FIU84_05010) rplM 1049194..1049622 (+) 429 WP_063544471.1 50S ribosomal protein L13 -
  FIU84_RS04995 (FIU84_05015) rpsI 1049637..1050029 (+) 393 WP_013981948.1 30S ribosomal protein S9 -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44565.56 Da        Isoelectric Point: 9.8649

>NTDB_id=393850 FIU84_RS04960 WP_063544459.1 1043948..1045168(+) (pilC) [Stutzerimonas frequens strain THAF7b]
MAEKALKTSVFTWEGTDRKGGKVKGELAGQSPALIKAQLRKQGINPLKVRKKAVSLFGAGKKIKPMDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVAAGNSFAGSLRKKPQYFDDLYCNLVESGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFESVFQGFGAELPAFTMMVVQLSRWLQEWWYIVFFALFIAAFLFK
HAYKRSEKFRDSLDRLLLKLPIVGDILYKSVVARYARTLSTTFAAGVPLVDALDSVAGATGNVVFRNAVAKIRTDVSSGV
QLNFSMRTTGIFPTMAIQMTAIGEESGALDEMLDKLASFYEAEVDNMVDNLTTLMEPLIMAVLGVLVGGLIIAMYLPIFQ
LGSVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=393850 FIU84_RS04960 WP_063544459.1 1043948..1045168(+) (pilC) [Stutzerimonas frequens strain THAF7b]
ATGGCGGAAAAAGCGTTGAAAACCAGTGTGTTCACCTGGGAAGGCACCGACAGAAAAGGTGGCAAGGTCAAGGGGGAGCT
GGCAGGCCAGAGCCCGGCACTGATCAAGGCGCAGCTCCGCAAGCAGGGTATCAATCCGCTGAAGGTTCGCAAGAAGGCTG
TTTCCTTGTTCGGGGCGGGCAAGAAAATCAAGCCGATGGACATCGCGCTGTTCACTCGGCAAATGGCCACCATGATGAAG
GCGGGCGTGCCGCTGCTGCAATCGTTCGACATCATTGGCGAGGGCTTCGATAACCCAAACATGCGCAAGCTGGTCGATGA
GATCAAGCAAGAGGTGGCGGCAGGCAACAGCTTTGCCGGCTCGCTGCGTAAGAAGCCGCAATATTTCGATGATCTGTATT
GCAATCTGGTCGAGTCTGGAGAGCAGTCCGGTGCGCTCGAAACGCTGCTGGACCGTGTTGCAACCTACAAGGAAAAAACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCGATGACCTATCCGATTGCGGTTATCGTGGTGGCAATCATCGTATCGGCGAT
TCTCTTGATTAAGGTGGTCCCTCAGTTCGAAAGTGTATTTCAGGGCTTCGGCGCGGAACTTCCAGCGTTCACCATGATGG
TGGTGCAGTTGTCACGTTGGCTGCAGGAGTGGTGGTATATCGTATTCTTTGCGTTGTTCATTGCTGCCTTTTTGTTCAAG
CATGCATATAAACGGTCGGAAAAGTTTCGTGACTCTCTTGATCGCCTTTTGCTCAAGCTGCCGATCGTTGGTGACATTCT
CTACAAGTCTGTAGTTGCGCGATATGCTCGCACATTGTCCACGACATTCGCGGCTGGTGTGCCCTTGGTTGATGCGTTGG
ACTCGGTAGCAGGTGCCACGGGCAACGTCGTGTTCCGTAATGCAGTAGCGAAAATCCGTACGGACGTATCGTCTGGTGTG
CAGCTCAATTTCTCCATGCGCACGACCGGCATTTTTCCCACCATGGCTATTCAAATGACCGCTATCGGCGAAGAGTCCGG
TGCGTTGGACGAGATGCTCGACAAACTGGCCAGTTTCTATGAGGCGGAGGTCGATAACATGGTGGATAACCTGACGACCC
TGATGGAACCGCTGATCATGGCCGTGCTCGGGGTTCTGGTCGGTGGCTTGATCATCGCGATGTATCTGCCGATCTTCCAG
TTGGGCAGTGTGGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

82.469

99.754

0.823
  pilC Acinetobacter baylyi ADP1

63.547

100

0.635
  pilC Acinetobacter baumannii D1279779

62.469

99.754

0.623
  pilC Legionella pneumophila strain ERS1305867

54.545

97.537

0.532
  pilG Neisseria gonorrhoeae MS11

45.522

99.015

0.451
  pilG Neisseria meningitidis 44/76-A

45.522

99.015

0.451
  pilC Vibrio cholerae strain A1552

43.35

100

0.434
  pilC Vibrio campbellii strain DS40M4

41

98.522

0.404

Multiple sequence alignment