Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FIU84_RS04955 Genome accession   NZ_CP045416
Coordinates   1042242..1043945 (+) Length   567 a.a.
NCBI ID   WP_152480902.1    Uniprot ID   -
Organism   Stutzerimonas frequens strain THAF7b     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1037242..1048945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU84_RS04930 (FIU84_04945) - 1038286..1039182 (-) 897 WP_152480899.1 ABC transporter ATP-binding protein -
  FIU84_RS04935 (FIU84_04950) - 1039179..1040018 (-) 840 WP_172974159.1 hypothetical protein -
  FIU84_RS04940 (FIU84_04955) - 1040020..1040829 (-) 810 WP_256365787.1 hypothetical protein -
  FIU84_RS04945 - 1041142..1041606 (-) 465 WP_152480900.1 hypothetical protein -
  FIU84_RS21720 (FIU84_04965) - 1041633..1041818 (-) 186 WP_152480901.1 type IV pilin protein -
  FIU84_RS04955 (FIU84_04970) pilB 1042242..1043945 (+) 1704 WP_152480902.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FIU84_RS04960 (FIU84_04975) pilC 1043948..1045168 (+) 1221 WP_063544459.1 type II secretion system F family protein Machinery gene
  FIU84_RS04965 (FIU84_04980) pilD 1045172..1046038 (+) 867 WP_152480903.1 A24 family peptidase Machinery gene
  FIU84_RS04970 (FIU84_04985) coaE 1046170..1046778 (+) 609 WP_152480904.1 dephospho-CoA kinase -
  FIU84_RS04975 (FIU84_04990) yacG 1046775..1046972 (+) 198 WP_102840588.1 DNA gyrase inhibitor YacG -
  FIU84_RS04980 (FIU84_04995) - 1046995..1047675 (-) 681 WP_102840589.1 energy-coupling factor ABC transporter permease -
  FIU84_RS04985 (FIU84_05005) - 1047714..1048750 (-) 1037 Protein_977 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62242.07 Da        Isoelectric Point: 5.2323

>NTDB_id=393849 FIU84_RS04955 WP_152480902.1 1042242..1043945(+) (pilB) [Stutzerimonas frequens strain THAF7b]
MNESAPLGGLARQLVQVGQLDEKTAQQAQQQALRNQVPLVTWLVQNNLVKSRVLVDIAGEQFGIPFFDLGALDKETQPKE
LVSEKLVRQHRALPLQRRGNKLYVAVSDPTNHQAISDIQFNTGLSVEAVLVEDDKLGEAIEKFFESATSSLEGLNDSELD
GLDIEVAGDAKEDDAAHNEADDAPVVRFVNKILLDAIKGGSSDLHFEPYEKSYRVRFRTDGMLHEVARPPVQLVSRISAR
LKVMASLDIAERRKPQDGRIKMKVSSTKSIDFRVNTLPTLWGEKIVMRILDSSSAKMGIDALGYEDDQKELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPSCKQEVDVPRDVLLSEGFP
EARIGSFKLYGPVGCDSCKGGYKGRVGIYEVVKITPALQRLIMEEGNSIDIALQARRDGFNDLRTSGLVKAMNGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=393849 FIU84_RS04955 WP_152480902.1 1042242..1043945(+) (pilB) [Stutzerimonas frequens strain THAF7b]
ATGAACGAGAGCGCTCCCCTCGGTGGCCTGGCCCGGCAGCTTGTGCAGGTCGGCCAGCTTGATGAAAAAACCGCTCAGCA
GGCGCAGCAGCAAGCCTTGCGTAACCAGGTGCCGTTGGTGACCTGGTTGGTGCAAAACAATCTCGTCAAGAGTCGAGTGC
TTGTCGATATCGCTGGCGAGCAGTTTGGCATTCCGTTCTTTGATCTGGGGGCGCTGGACAAAGAAACCCAGCCGAAGGAA
CTGGTCAGCGAGAAGCTGGTGCGCCAGCACCGCGCTCTGCCGTTGCAGCGACGAGGCAACAAGCTTTATGTGGCGGTGTC
CGATCCAACCAACCATCAGGCGATCAGTGATATTCAGTTCAATACGGGGCTTTCCGTTGAGGCTGTGCTGGTTGAGGACG
ACAAGCTCGGTGAGGCCATCGAAAAGTTCTTCGAGTCGGCGACCTCGTCCCTTGAAGGATTGAACGATTCCGAGTTGGAT
GGCTTGGATATCGAAGTGGCCGGTGACGCCAAGGAGGACGATGCGGCGCATAACGAAGCCGATGATGCGCCAGTGGTGCG
GTTTGTTAACAAGATTCTGCTCGATGCGATCAAGGGTGGTTCGTCCGATTTGCACTTCGAGCCTTATGAAAAGAGCTATC
GGGTGCGCTTTCGTACGGACGGCATGTTGCACGAGGTTGCCCGCCCGCCGGTCCAGTTGGTCAGCCGTATCTCAGCGCGT
CTGAAAGTAATGGCGAGTCTGGATATCGCCGAGCGGCGCAAACCGCAGGACGGGCGGATCAAGATGAAGGTCTCGAGTAC
CAAGTCGATCGATTTTCGCGTGAATACGCTACCCACTTTGTGGGGCGAGAAGATCGTTATGCGAATTTTGGATTCTTCCA
GTGCCAAGATGGGTATTGATGCGCTGGGGTATGAGGATGACCAGAAGGAGCTCTATCTAAGCGCGCTGAAGCAGCCGCAA
GGGATGATTCTGGTTACCGGCCCGACAGGCTCGGGCAAGACTGTCTCGCTTTATACCGGCCTGAATATTCTCAATACGGT
CGATGTGAATATCTCCACTGCCGAGGATCCGGTAGAGATCAATTTGGAAGGTATCAATCAGGTCAACGTAAATCCGAAGC
AGGGCATGGACTTTGCCCAGGCGCTTCGAGCGTTCCTGCGCCAGGATCCGGACATCATCATGGTGGGTGAGATTCGAGAT
CTCGAAACCGCCGAGATTGCCATCAAGGCAGCGCAGACCGGGCACATGGTGATGTCGACTCTGCATACCAATAGTGCTGC
GGAAACGCTTACTCGCTTGCGCAACATGGGTGTGCCGGCATTCAACATTGCCACATCGGTCAACCTGATCATTGCTCAAC
GGCTGGCTCGTAAGCTGTGCCCCAGCTGCAAGCAAGAAGTCGATGTGCCGCGAGATGTTCTGCTCAGCGAAGGATTTCCT
GAAGCGCGAATCGGTTCCTTCAAGCTTTACGGGCCGGTGGGGTGCGATAGCTGCAAGGGAGGCTACAAGGGGCGTGTCGG
TATTTATGAAGTGGTTAAAATCACGCCGGCCCTGCAGCGGCTTATCATGGAAGAAGGCAATTCCATCGATATCGCCTTGC
AGGCGCGTAGAGATGGGTTCAATGACCTGCGCACTTCGGGTCTGGTCAAGGCCATGAACGGCGTCACCAGCCTGGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.474

100

0.598
  pilB Acinetobacter baylyi ADP1

58.511

99.471

0.582
  pilB Legionella pneumophila strain ERS1305867

54.641

100

0.55
  pilB Vibrio cholerae strain A1552

51.636

97.002

0.501
  pilF Neisseria gonorrhoeae MS11

49.912

99.647

0.497
  pilB Vibrio campbellii strain DS40M4

50

99.118

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

51.282

96.296

0.494
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.755

93.474

0.381
  pilF Thermus thermophilus HB27

37.696

100

0.381
  pilB/pilB1 Synechocystis sp. PCC 6803

39.575

91.358

0.362

Multiple sequence alignment