Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   FIU95_RS14830 Genome accession   NZ_CP045369
Coordinates   3441792..3443006 (+) Length   404 a.a.
NCBI ID   WP_152454503.1    Uniprot ID   A0A5P9F0B2
Organism   Microbulbifer sp. THAF38     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3436792..3448006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU95_RS14815 (FIU95_15480) - 3437303..3438481 (+) 1179 WP_152454500.1 DNA polymerase Y family protein -
  FIU95_RS21630 (FIU95_15485) - 3439140..3439562 (-) 423 WP_152454501.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FIU95_RS14825 (FIU95_15490) pilB 3439957..3441669 (+) 1713 WP_152454502.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FIU95_RS14830 (FIU95_15495) pilC 3441792..3443006 (+) 1215 WP_152454503.1 type II secretion system F family protein Machinery gene
  FIU95_RS14835 (FIU95_15500) pilD 3443013..3443891 (+) 879 WP_152454504.1 A24 family peptidase Machinery gene
  FIU95_RS14840 (FIU95_15505) coaE 3444025..3444621 (+) 597 WP_152454505.1 dephospho-CoA kinase -
  FIU95_RS14845 (FIU95_15510) yacG 3444696..3444911 (+) 216 WP_152454506.1 DNA gyrase inhibitor YacG -
  FIU95_RS14850 (FIU95_15515) mutT 3444977..3445375 (-) 399 WP_152454507.1 8-oxo-dGTP diphosphatase MutT -
  FIU95_RS14855 (FIU95_15520) argJ 3445385..3446587 (-) 1203 WP_152454508.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 43523.29 Da        Isoelectric Point: 9.7671

>NTDB_id=393714 FIU95_RS14830 WP_152454503.1 3441792..3443006(+) (pilC) [Microbulbifer sp. THAF38]
MANATAVPYIYKGVDSKGAKVQGEINGTSPALVKAQLRKQGIIANRVQKKPKPLFGGGKKKVKPADIALFTRQMATMMKA
GVPLVQSFEIVADGLDNQGVKELIFKIRDEVASGTAFADALRKHPLYFDNLFCNLVASGEQSGALETMLDRIATYKEKTE
SLKAKIKKAMTYPIAVIVVAIIVTSILLIKVVPQFAQTFSGFGADLPAFTLLVVSMSEWMQANWFFALLATVISIGGTIE
AKKRNKNVADFFDRLILKIPILGQITYNSIVARFARTLSTTFAAGVPLIDALKSVAGATGNTIYQEATLKVRDAVATGIP
LNTALRTSGLYPAMLVQMTAIGEESGALDEMLGKAADYYEEAVDNMVDNLTTLLEPMIMAVLGILVGGLMIAMYLPIFQL
GQVV

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=393714 FIU95_RS14830 WP_152454503.1 3441792..3443006(+) (pilC) [Microbulbifer sp. THAF38]
ATGGCCAACGCCACAGCAGTCCCATATATCTACAAAGGGGTGGATAGCAAAGGAGCCAAGGTTCAGGGAGAAATTAATGG
CACCAGTCCAGCGCTGGTGAAGGCCCAGCTGCGCAAACAGGGGATTATTGCTAATCGCGTCCAGAAAAAGCCCAAACCTT
TATTTGGGGGAGGCAAGAAAAAAGTTAAACCCGCAGATATTGCCCTATTTACCAGGCAGATGGCTACCATGATGAAAGCT
GGGGTTCCTTTAGTGCAAAGCTTTGAAATTGTCGCCGATGGCTTAGACAACCAAGGAGTTAAAGAACTTATTTTTAAAAT
TCGAGACGAAGTTGCTTCAGGCACTGCCTTTGCCGATGCCCTTCGAAAACATCCTCTTTACTTTGATAATCTATTTTGCA
ACCTAGTCGCTTCCGGTGAACAATCCGGGGCACTTGAAACAATGCTAGATAGGATTGCCACTTATAAAGAAAAAACGGAG
TCACTTAAAGCTAAAATTAAGAAAGCGATGACCTATCCTATTGCTGTTATCGTTGTAGCTATTATTGTTACTTCTATTCT
ACTGATTAAAGTTGTTCCTCAATTTGCCCAAACATTCTCCGGCTTTGGTGCAGACCTGCCAGCCTTTACACTATTAGTTG
TAAGTATGTCAGAATGGATGCAAGCCAATTGGTTTTTCGCATTATTAGCGACGGTTATAAGTATTGGCGGCACTATTGAA
GCCAAAAAACGAAATAAAAATGTCGCCGATTTTTTTGATCGATTGATATTAAAAATTCCAATCTTAGGACAGATCACTTA
TAACTCCATTGTCGCACGCTTTGCTAGAACTCTCTCTACAACCTTCGCAGCGGGTGTCCCACTGATTGATGCGCTGAAGT
CAGTGGCCGGAGCAACAGGAAATACTATTTATCAGGAAGCAACCTTAAAAGTTAGGGACGCTGTAGCAACAGGAATTCCC
CTCAACACCGCACTACGTACGTCTGGGCTATACCCAGCTATGCTGGTGCAGATGACAGCAATCGGTGAAGAGTCCGGCGC
ATTGGATGAGATGCTGGGAAAGGCAGCAGATTATTACGAAGAAGCGGTAGACAATATGGTAGATAACCTTACCACCCTTT
TGGAGCCCATGATTATGGCCGTACTCGGTATCCTGGTGGGCGGCCTGATGATTGCAATGTACCTACCCATCTTCCAGCTG
GGCCAAGTTGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(274-396)

Type II secretion system (T2SS), protein F (T2SSF)

(70-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9F0B2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

64.141

98.02

0.629
  pilC Acinetobacter baylyi ADP1

61.713

98.267

0.606
  pilC Acinetobacter baumannii D1279779

60.705

98.267

0.597
  pilC Legionella pneumophila strain ERS1305867

56

99.01

0.554
  pilG Neisseria gonorrhoeae MS11

42.569

98.267

0.418
  pilG Neisseria meningitidis 44/76-A

42.317

98.267

0.416
  pilC Vibrio campbellii strain DS40M4

41.206

98.515

0.406
  pilC Vibrio cholerae strain A1552

41.414

98.02

0.406

Multiple sequence alignment