Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   FIU95_RS14835 Genome accession   NZ_CP045369
Coordinates   3443013..3443891 (+) Length   292 a.a.
NCBI ID   WP_152454504.1    Uniprot ID   -
Organism   Microbulbifer sp. THAF38     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3438013..3448891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU95_RS21630 (FIU95_15485) - 3439140..3439562 (-) 423 WP_152454501.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FIU95_RS14825 (FIU95_15490) pilB 3439957..3441669 (+) 1713 WP_152454502.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FIU95_RS14830 (FIU95_15495) pilC 3441792..3443006 (+) 1215 WP_152454503.1 type II secretion system F family protein Machinery gene
  FIU95_RS14835 (FIU95_15500) pilD 3443013..3443891 (+) 879 WP_152454504.1 A24 family peptidase Machinery gene
  FIU95_RS14840 (FIU95_15505) coaE 3444025..3444621 (+) 597 WP_152454505.1 dephospho-CoA kinase -
  FIU95_RS14845 (FIU95_15510) yacG 3444696..3444911 (+) 216 WP_152454506.1 DNA gyrase inhibitor YacG -
  FIU95_RS14850 (FIU95_15515) mutT 3444977..3445375 (-) 399 WP_152454507.1 8-oxo-dGTP diphosphatase MutT -
  FIU95_RS14855 (FIU95_15520) argJ 3445385..3446587 (-) 1203 WP_152454508.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 292 a.a.        Molecular weight: 32258.40 Da        Isoelectric Point: 7.5982

>NTDB_id=393715 FIU95_RS14835 WP_152454504.1 3443013..3443891(+) (pilD) [Microbulbifer sp. THAF38]
MPEYLFSYPALLIGSAFILGLVIGSFLNVVILRLPVMMEREYKRDFYSYFNTKPDLLEQKELDKPFNLVLPNSHCPKCKT
EIKPWQNIPVISYLLLRGKCGSCGTPIPMRYPFVELATGILTAVVVWQLGFTWQALAGCVFTWALVALTGIDFDKQLLPD
SITLPLLWGGLLINLWGIFVPPQDAVIGAIAGYLSLWLVFHIFKLVTGKEGMGAGDFKILAAIGAWFGWQMLPLVILLSA
AVGAIAGITWSLAVGRDRNLPIAFGPYLAGAGWIAMLWGEQIVGWYLNFSGL

Nucleotide


Download         Length: 879 bp        

>NTDB_id=393715 FIU95_RS14835 WP_152454504.1 3443013..3443891(+) (pilD) [Microbulbifer sp. THAF38]
ATGCCAGAATATTTATTTTCATATCCAGCGCTGCTAATAGGCAGCGCTTTTATTTTAGGCCTAGTCATAGGCAGCTTCCT
CAATGTGGTTATCCTGCGCCTGCCTGTAATGATGGAGCGGGAATACAAGCGGGATTTTTATAGCTACTTCAATACAAAAC
CAGACCTTTTGGAACAGAAAGAGCTGGATAAACCCTTTAACCTGGTACTACCCAACTCACACTGCCCTAAATGCAAAACC
GAGATTAAACCCTGGCAAAATATTCCTGTAATCAGTTATCTACTTTTGCGTGGTAAATGTGGTTCTTGCGGCACGCCCAT
TCCCATGCGTTACCCTTTCGTTGAGCTGGCTACTGGCATTCTCACCGCGGTAGTGGTTTGGCAGTTGGGCTTTACCTGGC
AGGCACTGGCTGGCTGTGTCTTTACCTGGGCCTTGGTGGCACTTACTGGTATCGACTTCGATAAGCAGTTATTACCAGAC
AGTATTACCTTACCTCTACTCTGGGGCGGCCTACTGATAAACCTGTGGGGTATTTTTGTTCCGCCGCAAGATGCCGTTAT
TGGGGCTATTGCCGGTTATCTTTCTTTGTGGCTGGTATTTCATATCTTCAAGCTGGTTACCGGTAAAGAGGGGATGGGAG
CTGGAGATTTCAAGATACTCGCAGCTATCGGTGCTTGGTTTGGCTGGCAGATGCTGCCTTTGGTAATTCTGCTCTCTGCT
GCTGTGGGCGCTATTGCCGGTATAACCTGGAGCCTGGCAGTTGGGCGAGATCGCAATCTGCCGATTGCCTTTGGCCCTTA
CTTGGCAGGTGCCGGCTGGATTGCCATGCTGTGGGGTGAGCAGATTGTCGGTTGGTACCTGAACTTTTCCGGTCTGTAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-128)

Type IV leader peptidase family (Peptidase_A24)

(140-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

52.158

95.205

0.497
  pilD Acinetobacter nosocomialis M2

51.799

95.205

0.493
  pilD Neisseria gonorrhoeae MS11

50.725

94.521

0.479
  pilD Vibrio cholerae strain A1552

48.11

99.658

0.479
  pilD Vibrio campbellii strain DS40M4

48.936

96.575

0.473

Multiple sequence alignment