Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FIU95_RS14825 Genome accession   NZ_CP045369
Coordinates   3439957..3441669 (+) Length   570 a.a.
NCBI ID   WP_152454502.1    Uniprot ID   -
Organism   Microbulbifer sp. THAF38     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3434957..3446669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU95_RS14805 (FIU95_15470) dcd 3435803..3436372 (-) 570 WP_152454498.1 dCTP deaminase -
  FIU95_RS14810 (FIU95_15475) imuA 3436592..3437290 (+) 699 WP_253868663.1 translesion DNA synthesis-associated protein ImuA -
  FIU95_RS14815 (FIU95_15480) - 3437303..3438481 (+) 1179 WP_152454500.1 DNA polymerase Y family protein -
  FIU95_RS21630 (FIU95_15485) - 3439140..3439562 (-) 423 WP_152454501.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FIU95_RS14825 (FIU95_15490) pilB 3439957..3441669 (+) 1713 WP_152454502.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FIU95_RS14830 (FIU95_15495) pilC 3441792..3443006 (+) 1215 WP_152454503.1 type II secretion system F family protein Machinery gene
  FIU95_RS14835 (FIU95_15500) pilD 3443013..3443891 (+) 879 WP_152454504.1 A24 family peptidase Machinery gene
  FIU95_RS14840 (FIU95_15505) coaE 3444025..3444621 (+) 597 WP_152454505.1 dephospho-CoA kinase -
  FIU95_RS14845 (FIU95_15510) yacG 3444696..3444911 (+) 216 WP_152454506.1 DNA gyrase inhibitor YacG -
  FIU95_RS14850 (FIU95_15515) mutT 3444977..3445375 (-) 399 WP_152454507.1 8-oxo-dGTP diphosphatase MutT -
  FIU95_RS14855 (FIU95_15520) argJ 3445385..3446587 (-) 1203 WP_152454508.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62375.21 Da        Isoelectric Point: 5.0810

>NTDB_id=393713 FIU95_RS14825 WP_152454502.1 3439957..3441669(+) (pilB) [Microbulbifer sp. THAF38]
MTTSPLSGLAKRLVADQVLNEATAISAIKAARRENQTFAQHVIEAKLVSAKELANIASLAFGTPLFELGSYNFELIPKDI
IDEKLISKHFTLPLYKRGGRLFVAVADPTNLAALDEINFSTGLNTDAVLVEADKLGKAIESYLSQNDDMSAGLGGVDDEE
LDGLDIDDNEPDTSNDGELGGDEAPVVRFVNKVLLDAIRTNASDIHFEPYEKSYRVRFRTDGVLHEIAKPPIQLASRLSA
RLKVMSRMDISEKRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKLGIDALGYEDEQKKIYMDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGINQVNVSNKVGLNFAEALRSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIATSVSVIIAQRLARRLCNECKKPVNLPSEVLEAEGF
SNVTIPQSEWSIYQPVGCEHCSKGYKGRVGVYEVVRITDGISRIIMEGGNSIQIADKAREEGFNNLRTSALRKVVMGITS
LEEANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=393713 FIU95_RS14825 WP_152454502.1 3439957..3441669(+) (pilB) [Microbulbifer sp. THAF38]
ATGACAACTTCCCCTCTTAGCGGCTTGGCAAAACGCTTGGTTGCAGACCAGGTGCTTAATGAAGCAACAGCAATTTCGGC
AATCAAAGCTGCTCGTAGAGAAAATCAAACTTTCGCCCAACATGTAATTGAGGCCAAACTGGTCTCAGCAAAAGAGCTTG
CCAACATCGCCTCCCTTGCTTTTGGCACCCCCCTTTTTGAGTTAGGAAGCTATAACTTTGAGCTGATTCCAAAAGATATC
ATTGATGAAAAACTGATCAGCAAACATTTTACGCTACCGCTTTACAAGCGAGGCGGTCGTCTCTTTGTTGCCGTAGCAGA
CCCAACCAATCTCGCCGCCTTAGATGAAATCAATTTCAGCACTGGCCTAAACACTGATGCAGTTCTAGTTGAAGCCGATA
AGCTCGGCAAAGCTATCGAAAGCTATCTATCTCAAAACGATGATATGAGTGCTGGGCTAGGGGGTGTTGACGATGAAGAA
CTGGACGGGCTAGATATCGACGATAATGAGCCAGATACCAGCAATGACGGTGAACTAGGTGGAGATGAAGCGCCGGTTGT
TCGATTCGTTAATAAAGTTCTATTGGACGCAATTCGTACAAATGCCTCTGATATTCACTTTGAACCCTATGAAAAATCTT
ACAGGGTACGCTTCCGAACCGATGGTGTACTACACGAAATTGCCAAGCCTCCAATCCAATTAGCCAGCCGACTTTCAGCT
CGCCTAAAAGTAATGTCCAGGATGGATATTTCCGAAAAGCGAGTGCCGCAGGATGGTCGCATTAAAATGAAGCTCTCCAA
AACCAAGGCAATTGATTTTCGTGTAAATAGCCTACCGACCTTGTGGGGAGAAAAGATTGTATTGCGCATACTGGATCCCT
CCTCTGCCAAGCTGGGCATTGATGCTCTTGGCTATGAGGATGAACAGAAAAAAATCTATATGGATGCACTGGCCCAACCA
CAAGGGATGATCCTGGTAACAGGCCCTACCGGCTCCGGTAAGACTGTCTCATTATATACAGGCCTCAATATTCTCAATAC
GCCTGAACGCAATATATCCACTGCTGAAGACCCTGTAGAAATCAATCTGGAGGGCATCAACCAGGTCAACGTATCCAATA
AGGTGGGCCTCAACTTTGCCGAGGCATTGCGCTCATTCCTACGACAAGACCCAGACATCGTAATGGTGGGGGAAATACGG
GATTTGGAGACAGCAGAAATCGCTATTAAAGCAGCACAAACCGGACACCTAGTGCTATCCACACTGCATACAAATTCAGC
ACCCGAGACCCTAACACGCCTAATGAATATGGGGGTACCTACTTTTAATATTGCCACCTCGGTCAGTGTGATTATTGCCC
AACGCCTTGCGCGGCGCTTATGCAACGAGTGCAAGAAGCCTGTAAATCTACCCAGCGAAGTACTTGAGGCTGAGGGCTTT
AGTAACGTTACTATCCCCCAGAGTGAGTGGAGCATCTATCAGCCGGTTGGATGCGAGCATTGCTCCAAGGGCTATAAGGG
GCGTGTGGGTGTGTATGAAGTGGTTCGCATTACCGACGGTATCTCAAGAATTATAATGGAAGGCGGCAACTCGATTCAGA
TCGCTGATAAAGCTAGAGAAGAAGGCTTTAACAATTTAAGAACCTCCGCTTTACGCAAAGTAGTAATGGGAATAACCAGT
CTCGAAGAGGCAAACCGGGTAACTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.908

100

0.612
  pilB Acinetobacter baylyi ADP1

60.315

100

0.605
  pilB Legionella pneumophila strain ERS1305867

53.968

99.474

0.537
  pilB Vibrio cholerae strain A1552

51.337

98.421

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

52.198

95.789

0.5
  pilB Vibrio campbellii strain DS40M4

50.442

99.123

0.5
  pilF Neisseria gonorrhoeae MS11

47.88

99.298

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

42.3

90

0.381

Multiple sequence alignment