Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FIV08_RS13115 Genome accession   NZ_CP045324
Coordinates   2825203..2826915 (+) Length   570 a.a.
NCBI ID   WP_152438648.1    Uniprot ID   -
Organism   Marinobacter sp. THAF197a     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2814314..2824231 2825203..2826915 flank 972


Gene organization within MGE regions


Location: 2814314..2826915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIV08_RS13075 (FIV08_13215) - 2814314..2815426 (+) 1113 WP_152438641.1 glycosyltransferase -
  FIV08_RS13080 (FIV08_13220) - 2815350..2816606 (-) 1257 WP_152438642.1 lipopolysaccharide biosynthesis protein -
  FIV08_RS13085 (FIV08_13225) asnB 2816871..2818736 (-) 1866 WP_152438643.1 asparagine synthase (glutamine-hydrolyzing) -
  FIV08_RS13090 (FIV08_13235) - 2819043..2820152 (-) 1110 WP_152438644.1 glycosyltransferase -
  FIV08_RS13095 (FIV08_13240) - 2820209..2821384 (-) 1176 WP_152438645.1 glycosyltransferase family 4 protein -
  FIV08_RS13100 (FIV08_13245) - 2821430..2822284 (-) 855 WP_216646150.1 glycosyltransferase family 2 protein -
  FIV08_RS13105 (FIV08_13250) - 2822354..2824258 (-) 1905 WP_152438647.1 tetratricopeptide repeat protein -
  FIV08_RS13110 (FIV08_13255) pilA2 2824362..2824811 (-) 450 WP_323847456.1 pilin Machinery gene
  FIV08_RS13115 (FIV08_13260) pilB 2825203..2826915 (+) 1713 WP_152438648.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62643.73 Da        Isoelectric Point: 4.9547

>NTDB_id=393453 FIV08_RS13115 WP_152438648.1 2825203..2826915(+) (pilB) [Marinobacter sp. THAF197a]
MASSNRNVTLTGLARRFVDDGLLDEETAKDAFLQASQNRIPLITYLTQNNLADSSKLAFSAAMEFGVSVLDLDSFLPEMM
PEKVVDEKLVRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDAKLRTAIDKYLESHDTTMGDLDDA
DLEGVETEGGDQDDDGAVSATEVDDAPIVKYVNKMLLDAIRGGASDIHFEPYEKIYRVRYRTDGILKEISRPSIKLAPKI
SARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKQLYMDALA
QPQGMILVTGPTGSGKTVSLYTGLNILNTPGINISTAEDPAEINLEGINQVNVNTKVGLGFAEALRAFLRQDPDVIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCSCKQPADVPKDVLLKEG
FTQEQIDTGFTLYRPKGCDKCSNGYKGRVGIYEVVKITEELASMIMEEASSIKIAKQAQAEGFRNLRQSALLKVMEGVTS
LEEANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=393453 FIV08_RS13115 WP_152438648.1 2825203..2826915(+) (pilB) [Marinobacter sp. THAF197a]
ATGGCGAGCAGCAACCGAAACGTAACCCTGACGGGCCTGGCCCGTCGATTTGTGGATGACGGGCTTCTTGACGAAGAAAC
CGCCAAAGATGCCTTTCTTCAGGCCTCCCAGAACCGCATCCCACTGATTACCTACCTTACCCAGAACAATCTGGCAGATA
GCTCCAAATTGGCATTTTCTGCTGCGATGGAATTTGGCGTCTCGGTACTGGACCTGGATTCGTTCCTACCGGAAATGATG
CCTGAGAAGGTGGTTGACGAAAAGTTGGTGCGGAAGCACAACGCCCTGCCCTTGTATAAGAGAGGTAATCGGCTATTCAT
CGCAGTATCAGACCCAACAAACATTCAGGCCTTGGATGAGATCAAGTTCAACACAGGATTGAGCACTGACGCGATTCTTG
TGGACGATGCCAAGCTGCGCACAGCCATCGACAAGTACCTTGAATCCCATGACACTACCATGGGTGATCTCGACGATGCT
GATCTTGAGGGCGTCGAAACCGAGGGCGGCGACCAGGACGACGACGGTGCCGTCAGCGCTACGGAAGTAGATGATGCACC
TATTGTTAAGTATGTTAACAAGATGTTGCTTGATGCCATCCGCGGCGGCGCATCCGATATTCACTTTGAGCCATACGAAA
AGATCTACCGGGTTCGCTACCGGACAGATGGAATCCTGAAAGAGATATCTCGCCCATCCATTAAACTTGCACCGAAGATC
TCTGCCCGGGTGAAGATCATGGCCCAACTGGATATCTCGGAGCGCCGCGTACCTCAGGACGGTCGGATCAAGATGAAGCT
GTCCAAGACCAAAGCCATCGACTTCCGGGTGAATACTCTGCCCACCCTTTGGGGCGAAAAAATTGTTCTTCGGATTCTGG
ACCCAAGCCAGGCCAAGCTGGGCATTGATGTCTTAGGTTATGAGGAAGACCAGAAGCAACTATATATGGACGCCCTAGCG
CAACCCCAGGGTATGATTCTGGTAACCGGGCCGACCGGCTCCGGTAAAACGGTATCCCTCTACACCGGCTTGAACATTCT
GAACACACCGGGCATCAACATTTCCACGGCGGAAGATCCGGCGGAGATCAACTTGGAAGGCATCAACCAGGTCAACGTGA
ACACCAAGGTAGGCTTGGGATTTGCGGAAGCGTTGCGGGCATTCTTGCGGCAGGACCCTGACGTAATCATGGTGGGTGAG
ATCCGGGATCTGGAAACCGCTAACATTGCAATCAAAGCCGCACAGACGGGGCACTTGGTTCTGTCTACGCTGCACACTAA
CAGCGCTGCCGAAACCCTCACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATTGCCACCTCGGTGAGCTTGATCA
TTGCCCAGCGCCTTGGTCGGCGCCTGTGCAGCTGCAAACAGCCTGCAGACGTGCCCAAAGACGTACTTTTGAAGGAAGGG
TTCACACAAGAACAAATTGATACAGGCTTCACGTTGTACCGCCCCAAGGGCTGTGATAAATGCTCTAATGGATATAAAGG
CCGCGTCGGTATTTACGAGGTGGTGAAGATTACTGAAGAGCTGGCAAGCATGATTATGGAAGAAGCCAGTTCTATAAAAA
TTGCAAAACAAGCGCAGGCAGAAGGCTTCCGCAACCTGAGGCAATCCGCCCTTCTGAAAGTAATGGAAGGAGTGACCAGC
CTTGAGGAAGCCAACCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.343

100

0.575
  pilB Acinetobacter baylyi ADP1

57.549

98.772

0.568
  pilB Legionella pneumophila strain ERS1305867

53.565

100

0.54
  pilB Vibrio parahaemolyticus RIMD 2210633

52.065

97.719

0.509
  pilB Vibrio campbellii strain DS40M4

50.709

98.947

0.502
  pilB Vibrio cholerae strain A1552

53.757

91.053

0.489
  pilF Neisseria gonorrhoeae MS11

48.41

99.298

0.481
  pilB/pilB1 Synechocystis sp. PCC 6803

37.236

100

0.402
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.544

92.281

0.365
  pilF Thermus thermophilus HB27

37.184

97.193

0.361

Multiple sequence alignment