Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GCS73_RS08495 Genome accession   NZ_CP045308
Coordinates   1853559..1854779 (-) Length   406 a.a.
NCBI ID   WP_003631624.1    Uniprot ID   D3HSR8
Organism   Legionella longbeachae strain B41211CHC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1848559..1859779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCS73_RS08465 (GCS73_08395) - 1848915..1849469 (-) 555 WP_003631631.1 HPF/RaiA family ribosome-associated protein -
  GCS73_RS08470 (GCS73_08400) - 1849521..1849970 (-) 450 WP_003631630.1 CBS domain-containing protein -
  GCS73_RS08475 (GCS73_08405) - 1850269..1850472 (+) 204 WP_003631628.1 DUF1328 family protein -
  GCS73_RS08480 (GCS73_08410) - 1850561..1851772 (-) 1212 WP_003631627.1 sterol desaturase family protein -
  GCS73_RS08485 (GCS73_08415) - 1851811..1852566 (-) 756 WP_003631626.1 HAD-IA family hydrolase -
  GCS73_RS08490 (GCS73_08420) - 1852587..1853447 (-) 861 WP_003631625.1 prepilin peptidase -
  GCS73_RS08495 (GCS73_08425) pilC 1853559..1854779 (-) 1221 WP_003631624.1 type II secretion system F family protein Machinery gene
  GCS73_RS08500 (GCS73_08430) pilB 1854792..1856510 (-) 1719 WP_003631623.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GCS73_RS08505 (GCS73_08435) - 1856853..1857590 (+) 738 WP_012979093.1 methyltransferase domain-containing protein -
  GCS73_RS08510 (GCS73_08440) - 1857637..1858218 (-) 582 WP_003631620.1 hypothetical protein -
  GCS73_RS08515 (GCS73_08445) - 1858438..1858764 (+) 327 WP_003631619.1 DUF1820 family protein -
  GCS73_RS08520 (GCS73_08450) - 1858808..1859368 (+) 561 WP_003631618.1 hypoxanthine-guanine phosphoribosyltransferase -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44593.63 Da        Isoelectric Point: 10.1093

>NTDB_id=393357 GCS73_RS08495 WP_003631624.1 1853559..1854779(-) (pilC) [Legionella longbeachae strain B41211CHC]
MKKNANTTLTFHYTGVNKASQKINGDIEARSLALAKVELRRQGIIISKIVKKRSPILKRNSKKVKSSDITVFSRQLATMI
ESGIPLVQSFDIVAKGQTNTRLKELIEQIKHDVETGLTLSEALKKHPSHFNDLFCNLVDAGEKSGSLDIMLNKIATYKEK
IETIKKKIKKALTYPMAVIVVALIVTAGLLIFVVPQFEALFKGFGADLPMMTKTVVELSKFFQSYWYLIFGSLIGIVYAF
IYAVKHSSQFAQNVDKTLLKIPVIGPIIEKAAIARFARTLSITFAAGLPLVEALKSVAGATGNIIFSNATNKIRDEVSTG
QQMNKAMENTQLFPNMVIQMVAIGEESGALERMLSKVADFYEEDVDNAVDSLSSLLEPIIMTILGVLVGGLVVSMYLPIF
KLGAAI

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=393357 GCS73_RS08495 WP_003631624.1 1853559..1854779(-) (pilC) [Legionella longbeachae strain B41211CHC]
ATGAAAAAAAATGCAAATACTACATTAACTTTTCATTACACAGGAGTCAATAAAGCTTCGCAAAAAATCAATGGGGATAT
AGAAGCTAGAAGCCTTGCTTTAGCTAAAGTAGAACTACGTAGACAAGGGATTATTATTAGTAAAATTGTAAAAAAACGAT
CCCCTATTTTAAAACGAAATAGCAAAAAAGTTAAATCTTCAGACATCACGGTGTTTAGCCGTCAATTAGCGACAATGATT
GAATCAGGTATCCCTTTAGTTCAATCTTTTGATATTGTAGCTAAAGGGCAAACTAACACACGCCTCAAAGAGTTAATTGA
GCAAATTAAGCACGATGTTGAAACAGGATTAACACTTTCTGAAGCCTTAAAAAAACATCCCTCTCATTTTAATGATTTAT
TTTGCAATTTAGTCGATGCTGGTGAAAAATCAGGCTCTCTCGATATCATGTTGAATAAAATCGCTACTTATAAAGAAAAA
ATAGAAACCATAAAAAAGAAAATCAAAAAAGCATTAACCTACCCTATGGCAGTGATCGTTGTAGCTCTTATTGTTACTGC
AGGATTACTTATTTTTGTGGTGCCCCAGTTTGAAGCATTATTTAAAGGTTTTGGGGCAGATTTACCCATGATGACAAAAA
CCGTAGTAGAACTTTCTAAATTTTTTCAATCCTATTGGTATCTTATATTTGGATCATTAATTGGAATTGTTTACGCTTTT
ATTTATGCAGTAAAACACTCCTCGCAATTTGCTCAAAATGTAGATAAAACATTATTAAAAATTCCTGTAATTGGACCCAT
TATTGAAAAAGCTGCGATTGCCCGTTTTGCAAGAACACTATCAATTACCTTCGCCGCAGGTTTGCCTCTGGTTGAAGCAC
TTAAATCAGTTGCAGGTGCTACAGGGAACATTATCTTTTCAAATGCAACAAATAAAATCAGAGATGAAGTTTCCACTGGA
CAACAAATGAACAAAGCCATGGAAAACACTCAATTATTCCCGAATATGGTAATTCAAATGGTTGCCATAGGCGAAGAATC
AGGCGCATTGGAAAGAATGCTAAGTAAAGTTGCTGATTTTTATGAAGAAGATGTTGATAATGCAGTTGACTCCCTCAGTA
GTTTACTAGAACCTATAATTATGACTATTTTGGGAGTTCTAGTGGGAGGCCTTGTAGTTTCAATGTATTTGCCAATTTTT
AAATTAGGTGCTGCAATATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D3HSR8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

77.094

100

0.771
  pilC Acinetobacter baumannii D1279779

53.086

99.754

0.53
  pilC Pseudomonas stutzeri DSM 10701

51.97

100

0.52
  pilC Acinetobacter baylyi ADP1

51.111

99.754

0.51
  pilG Neisseria gonorrhoeae MS11

44.146

100

0.446
  pilG Neisseria meningitidis 44/76-A

44.146

100

0.446
  pilC Vibrio cholerae strain A1552

42.857

96.552

0.414
  pilC Vibrio campbellii strain DS40M4

39.163

100

0.392
  pilC Thermus thermophilus HB27

35.731

100

0.367

Multiple sequence alignment