Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GCS73_RS08500 Genome accession   NZ_CP045308
Coordinates   1854792..1856510 (-) Length   572 a.a.
NCBI ID   WP_003631623.1    Uniprot ID   -
Organism   Legionella longbeachae strain B41211CHC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1849792..1861510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCS73_RS08475 (GCS73_08405) - 1850269..1850472 (+) 204 WP_003631628.1 DUF1328 family protein -
  GCS73_RS08480 (GCS73_08410) - 1850561..1851772 (-) 1212 WP_003631627.1 sterol desaturase family protein -
  GCS73_RS08485 (GCS73_08415) - 1851811..1852566 (-) 756 WP_003631626.1 HAD-IA family hydrolase -
  GCS73_RS08490 (GCS73_08420) - 1852587..1853447 (-) 861 WP_003631625.1 prepilin peptidase -
  GCS73_RS08495 (GCS73_08425) pilC 1853559..1854779 (-) 1221 WP_003631624.1 type II secretion system F family protein Machinery gene
  GCS73_RS08500 (GCS73_08430) pilB 1854792..1856510 (-) 1719 WP_003631623.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GCS73_RS08505 (GCS73_08435) - 1856853..1857590 (+) 738 WP_012979093.1 methyltransferase domain-containing protein -
  GCS73_RS08510 (GCS73_08440) - 1857637..1858218 (-) 582 WP_003631620.1 hypothetical protein -
  GCS73_RS08515 (GCS73_08445) - 1858438..1858764 (+) 327 WP_003631619.1 DUF1820 family protein -
  GCS73_RS08520 (GCS73_08450) - 1858808..1859368 (+) 561 WP_003631618.1 hypoxanthine-guanine phosphoribosyltransferase -
  GCS73_RS08525 (GCS73_08455) - 1859700..1860395 (-) 696 WP_003631616.1 putative DNA-binding domain-containing protein -
  GCS73_RS08530 (GCS73_08460) - 1860382..1861415 (-) 1034 Protein_1596 DUF692 domain-containing protein -
  GCS73_RS08535 - 1861351..1861500 (-) 150 WP_003631613.1 hypothetical protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 63374.18 Da        Isoelectric Point: 6.0426

>NTDB_id=393358 GCS73_RS08500 WP_003631623.1 1854792..1856510(-) (pilB) [Legionella longbeachae strain B41211CHC]
MLGSNDFKLYGIGQLFVLEKLLEKSEAIEYYKLADAEKISLVQYLVKNNILSAIQIAHTASQNFGVPMLDLDCIDIESLP
TSLVNEALIRRHSMIPLFCRGNHLYLATDDPSKQTSLKEIQFQTGLNTYAIVVETDKLNVLIDHLLTTKENQCLSEFVGD
TNEMDGLIINEEEEHELNAPASITEDAPIVKFVNKILLDAIKQGASDIHFEPYDKEYRIRYRQDGILHEIATPPARLAAR
ITARIKIMSCLDISERRIPQDGGFKMKLSKTRTIDFRVSTCPTIAGEKVVTRILDPGSVKLGIEALGFSPLQKDHFIHAI
ERPQGMILVTGPTGSGKTVTLYTALNILNTKEVNISTAEDPVEIKMHGINQVNINPKAGLTFSNALRSFLRQDPDIIMVG
EIRDLETAEIAVKASQTGHLVLSTLHTNSASETLNRLVNMGIPTFNITSSVTLIIAQRLARKLCEHCKILRDDFTKSGLL
ELGFTEADVQEIKLYKAVGCNKCTAGYRGRIGLFEVLPMTKTIGQLIMSGGNALDILKAAQDEGMLTIYQSGLEKIKHGI
TTIEEVNRVTVD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=393358 GCS73_RS08500 WP_003631623.1 1854792..1856510(-) (pilB) [Legionella longbeachae strain B41211CHC]
ATGTTAGGAAGCAATGATTTTAAATTATATGGGATAGGTCAACTTTTTGTCTTAGAGAAACTTTTAGAAAAATCTGAGGC
CATAGAGTACTATAAGCTTGCCGATGCTGAAAAAATCTCATTAGTTCAATACCTTGTTAAAAACAACATATTATCAGCCA
TACAAATTGCCCACACAGCATCGCAAAATTTTGGTGTGCCTATGCTGGATCTCGATTGTATTGATATAGAATCACTCCCT
ACAAGTTTAGTGAATGAGGCATTAATTCGGCGTCATTCAATGATTCCGTTATTTTGTCGAGGAAATCATTTATATTTAGC
CACTGACGATCCAAGCAAACAAACTTCATTGAAAGAGATCCAATTTCAAACTGGACTTAATACTTATGCGATCGTAGTAG
AAACAGATAAGCTCAATGTCCTAATTGATCATTTGCTCACCACTAAAGAAAACCAATGTTTATCCGAGTTTGTTGGTGAT
ACTAATGAGATGGATGGACTCATTATTAATGAAGAGGAAGAGCATGAACTCAATGCGCCCGCCTCGATAACAGAAGATGC
TCCAATCGTCAAATTTGTCAATAAAATTCTTCTAGATGCCATTAAACAAGGAGCGTCTGATATTCACTTTGAACCCTATG
ATAAAGAATATCGTATACGATACCGCCAAGACGGAATTTTGCATGAAATAGCAACTCCACCTGCAAGATTAGCAGCCAGA
ATTACTGCCCGCATCAAAATAATGTCTTGTTTGGATATTTCTGAAAGGCGTATCCCACAAGATGGTGGTTTTAAAATGAA
ATTATCTAAAACAAGAACCATTGATTTTCGTGTTAGCACCTGCCCTACCATTGCTGGTGAAAAAGTAGTAACTCGTATTC
TTGATCCAGGTTCTGTTAAATTGGGGATTGAAGCTTTAGGTTTTAGCCCTTTGCAAAAAGATCATTTTATCCATGCAATT
GAACGTCCCCAAGGCATGATTTTGGTTACTGGACCGACTGGAAGTGGCAAAACCGTAACCTTATATACCGCATTAAATAT
ACTTAATACCAAGGAAGTGAATATTTCCACCGCGGAGGATCCTGTAGAAATTAAAATGCATGGTATTAATCAAGTGAACA
TAAATCCTAAGGCAGGATTAACATTCTCTAATGCCCTACGATCTTTTTTGCGCCAAGACCCCGACATCATTATGGTGGGA
GAGATACGTGACTTGGAAACAGCAGAAATAGCGGTCAAAGCATCACAAACCGGGCATTTAGTCCTTTCGACCTTGCATAC
GAATAGTGCATCTGAAACATTAAATCGCTTGGTGAATATGGGAATTCCAACATTTAATATCACCAGTTCCGTCACTCTCA
TAATTGCGCAGCGTCTCGCCCGTAAATTATGTGAACATTGTAAAATTTTAAGAGATGACTTCACTAAATCAGGCTTACTC
GAATTAGGATTTACAGAAGCAGACGTACAAGAAATCAAGTTATACAAAGCAGTAGGTTGTAATAAATGTACCGCTGGTTA
CCGAGGAAGAATAGGATTATTTGAAGTATTACCCATGACTAAAACCATTGGTCAATTAATCATGTCAGGAGGTAATGCCC
TTGATATTCTAAAAGCGGCACAAGATGAAGGCATGCTGACTATTTACCAATCTGGGCTTGAAAAGATAAAACATGGTATT
ACAACTATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

78.147

100

0.781
  pilB Acinetobacter baumannii D1279779

52.205

99.126

0.517
  pilB Acinetobacter baylyi ADP1

52.239

93.706

0.49
  pilB Vibrio cholerae strain A1552

48.794

94.231

0.46
  pilB Vibrio parahaemolyticus RIMD 2210633

48.168

95.455

0.46
  pilB Vibrio campbellii strain DS40M4

48.045

93.881

0.451
  pilF Neisseria gonorrhoeae MS11

45.133

98.776

0.446
  pilF Thermus thermophilus HB27

39.439

93.531

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.024

93.182

0.364

Multiple sequence alignment