Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GCK66_RS02205 Genome accession   NZ_CP045220
Coordinates   471150..472391 (-) Length   413 a.a.
NCBI ID   WP_206818892.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain Aer_Brac66     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 466150..477391
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCK66_RS02175 (GCK66_02200) - 466991..468175 (+) 1185 WP_206818896.1 tetratricopeptide repeat protein -
  GCK66_RS02180 (GCK66_02205) mutT 468184..468597 (+) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  GCK66_RS02185 (GCK66_02210) yacG 468658..468852 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  GCK66_RS02190 (GCK66_02215) zapD 468862..469584 (-) 723 WP_206818895.1 cell division protein ZapD -
  GCK66_RS02195 (GCK66_02220) coaE 469622..470236 (-) 615 WP_206818894.1 dephospho-CoA kinase -
  GCK66_RS02200 (GCK66_02225) pilD 470255..471127 (-) 873 WP_206818893.1 A24 family peptidase Machinery gene
  GCK66_RS02205 (GCK66_02230) pilC 471150..472391 (-) 1242 WP_206818892.1 type II secretion system F family protein Machinery gene
  GCK66_RS02210 (GCK66_02235) pilB 472507..474213 (-) 1707 WP_029302067.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  GCK66_RS02215 (GCK66_02240) tapA 474220..474636 (-) 417 WP_042066959.1 type IVa pilus major pilin TapA -
  GCK66_RS02220 (GCK66_02250) nadC 474959..475816 (-) 858 WP_206818891.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GCK66_RS02225 (GCK66_02255) - 475822..476292 (-) 471 WP_206818890.1 retropepsin-like aspartic protease -
  GCK66_RS02230 (GCK66_02260) ampD 476440..477012 (+) 573 WP_206818889.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45468.60 Da        Isoelectric Point: 9.8637

>NTDB_id=392764 GCK66_RS02205 WP_206818892.1 471150..472391(-) (pilC) [Aeromonas hydrophila strain Aer_Brac66]
MATLTQKQNAPKKVFAFRWSGVNRKGQKVSGELQADSINTVKAELRKQGVNVTKVSKKSQGIFSKGGAKIKPMDIAVVSR
QITTMLSAGVPLVQSLQIIARSHEKAAMRELMGQIAADVETGTPMSEALRRHPRHFDDLYCDLVEAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPTMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPIFTQFVIGISRFMQNWWYVIFGSIA
LSIFLYVRAWRASQKVKDNTDKFILTIPVVGMILHKAAMARFARTLSTTFSAGIPLVDALVSAAGASGNYVYRTAVMAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVATIFEQEVDDLVDGLTSLLEPLIMVVLGVLVGGMVVA
MYLPIFKLGSVIH

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=392764 GCK66_RS02205 WP_206818892.1 471150..472391(-) (pilC) [Aeromonas hydrophila strain Aer_Brac66]
ATGGCAACGCTAACCCAGAAACAGAATGCCCCCAAGAAAGTCTTCGCCTTTCGCTGGAGCGGGGTTAATCGCAAGGGCCA
GAAGGTCTCCGGCGAATTGCAGGCCGACAGCATCAACACGGTCAAGGCAGAGCTGCGCAAACAGGGTGTCAACGTCACCA
AGGTGAGCAAGAAGAGCCAGGGGATCTTCTCCAAGGGCGGCGCCAAGATCAAGCCGATGGACATCGCCGTCGTCTCCCGC
CAGATCACCACCATGTTGTCAGCCGGTGTACCTCTGGTCCAGAGCCTGCAGATCATCGCTCGCAGCCACGAGAAGGCGGC
CATGCGCGAGCTCATGGGCCAGATTGCCGCCGATGTGGAGACCGGCACGCCCATGTCCGAGGCACTGCGCCGCCATCCCC
GTCACTTTGACGATCTCTATTGCGATCTGGTGGAGGCTGGCGAGCAGTCCGGCGCTCTGGAGACCATCTACGATCGCATC
GCCACCTATCGCGAGAAATCGGAAGCACTCAAGTCCAAGATCAAGAAAGCCATGTTTTACCCCACCATGGTCATCCTGGT
GGCCATTGTCGTTACCTCCATCCTGCTACTGTTTGTCATTCCCCAGTTCGAGGACATCTTCAAGAGCTTCGGTGCCGAGC
TGCCCATCTTTACCCAGTTTGTCATCGGCATCTCCCGTTTCATGCAGAACTGGTGGTATGTGATCTTTGGCAGCATAGCG
CTTTCCATCTTTCTCTACGTGCGCGCCTGGCGCGCCTCCCAGAAGGTCAAGGACAACACCGACAAGTTCATACTGACCAT
CCCCGTGGTCGGCATGATATTGCACAAGGCCGCCATGGCCCGTTTCGCCCGTACCCTCTCCACCACCTTCTCTGCCGGTA
TCCCGCTGGTAGATGCCTTGGTTTCTGCAGCTGGCGCCTCCGGCAACTATGTCTACCGTACTGCCGTCATGGCCATTCGC
AACGAGGTGGTGGCCGGCATGCAGATCAACGTGGCCATGCGCACCGTCGATCTGTTCCCGGACATGGTGATCCAGATGGT
GATGATCGGCGAGGAGTCTGGCGCCATCGATGATATGCTCTCCAAGGTCGCCACCATCTTCGAACAGGAGGTGGACGATC
TGGTCGATGGCCTCACCAGCCTGCTGGAACCTCTCATCATGGTGGTGCTGGGGGTTCTGGTCGGCGGCATGGTGGTCGCC
ATGTATCTGCCCATCTTCAAACTGGGGTCGGTGATCCACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.566

95.884

0.542
  pilC Acinetobacter baumannii D1279779

53.75

96.852

0.521
  pilC Acinetobacter baylyi ADP1

52.141

96.126

0.501
  pilC Legionella pneumophila strain ERS1305867

51.111

98.063

0.501
  pilC Vibrio cholerae strain A1552

47.487

96.368

0.458
  pilC Vibrio campbellii strain DS40M4

46.348

96.126

0.446
  pilG Neisseria gonorrhoeae MS11

39.552

97.337

0.385
  pilG Neisseria meningitidis 44/76-A

39.303

97.337

0.383
  pilC Thermus thermophilus HB27

37.5

96.852

0.363

Multiple sequence alignment