Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   HO399_RS04490 Genome accession   NZ_CP053599
Coordinates   977604..978368 (-) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli strain NEBExpress     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 972604..983368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HO399_RS04480 (HO399_04480) nikR 976394..976795 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  HO399_RS04485 (HO399_04485) nikE 976801..977607 (-) 807 WP_000173665.1 nickel import ATP-binding protein NikE -
  HO399_RS04490 (HO399_04490) amiE 977604..978368 (-) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  HO399_RS04495 (HO399_04495) nikC 978368..979201 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  HO399_RS04500 (HO399_04500) nikB 979198..980142 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  HO399_RS04505 (HO399_04505) nikA 980142..981716 (-) 1575 WP_000953348.1 nickel ABC transporter substrate-binding protein -
  HO399_RS04510 (HO399_04510) acpT 981827..982414 (-) 588 WP_000285773.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=392299 HO399_RS04490 WP_001136236.1 977604..978368(-) (amiE) [Escherichia coli strain NEBExpress]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=392299 HO399_RS04490 WP_001136236.1 977604..978368(-) (amiE) [Escherichia coli strain NEBExpress]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398

Multiple sequence alignment