Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   D1010_RS00305 Genome accession   NZ_CP045143
Coordinates   66428..66865 (+) Length   145 a.a.
NCBI ID   WP_027828809.1    Uniprot ID   A0A0R1XHW9
Organism   Schleiferilactobacillus harbinensis strain M1     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 37492..111380 66428..66865 within 0


Gene organization within MGE regions


Location: 37492..111380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1010_RS00175 (D1010_00175) - 37522..38052 (+) 531 WP_152259980.1 hypothetical protein -
  D1010_RS00180 (D1010_00180) - 38049..38516 (+) 468 WP_152259981.1 zinc ribbon domain-containing protein -
  D1010_RS00185 (D1010_00185) - 38681..39538 (+) 858 WP_193312131.1 heparinase II/III family protein -
  D1010_RS00190 (D1010_00190) - 39493..40626 (+) 1134 WP_152259983.1 heparinase II/III family protein -
  D1010_RS00195 (D1010_00195) - 40852..41667 (+) 816 WP_146993935.1 hypothetical protein -
  D1010_RS00200 (D1010_00200) - 41779..42786 (-) 1008 WP_152259984.1 serine hydrolase -
  D1010_RS00205 (D1010_00205) - 42803..44632 (-) 1830 WP_152259985.1 DMT family transporter -
  D1010_RS00210 (D1010_00210) - 44809..45551 (+) 743 Protein_41 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
  D1010_RS00215 (D1010_00215) - 45557..46870 (+) 1314 WP_152259986.1 APC family permease -
  D1010_RS00220 (D1010_00220) - 46875..47684 (+) 810 WP_152259987.1 GNAT family N-acetyltransferase -
  D1010_RS00225 (D1010_00225) - 47752..48171 (-) 420 WP_226911032.1 YjdF family protein -
  D1010_RS00230 (D1010_00230) - 48439..48834 (+) 396 WP_152259989.1 hypothetical protein -
  D1010_RS00235 (D1010_00235) arsC 48876..49283 (+) 408 WP_152259990.1 arsenate reductase (thioredoxin) -
  D1010_RS18860 - 49465..49524 (-) 60 WP_220095855.1 hypothetical protein -
  D1010_RS00240 (D1010_00240) - 49773..51011 (-) 1239 WP_152259991.1 S41 family peptidase -
  D1010_RS00245 (D1010_00245) - 51116..52015 (+) 900 WP_193312132.1 helix-turn-helix domain-containing protein -
  D1010_RS00250 (D1010_00250) - 52181..53815 (+) 1635 WP_226911033.1 ABC-F family ATP-binding cassette domain-containing protein -
  D1010_RS00255 (D1010_00255) - 54178..55383 (+) 1206 WP_152259993.1 RNA-guided endonuclease TnpB family protein -
  D1010_RS18865 - 55615..55665 (+) 51 WP_405127572.1 hypothetical protein -
  D1010_RS00260 (D1010_00260) abc-f 55836..57419 (-) 1584 WP_152259994.1 ribosomal protection-like ABC-F family protein -
  D1010_RS00265 (D1010_00265) recQ 57759..59582 (+) 1824 WP_405127553.1 DNA helicase RecQ -
  D1010_RS00270 (D1010_00270) - 59545..61164 (-) 1620 WP_193312133.1 ABC transporter ATP-binding protein -
  D1010_RS00275 (D1010_00275) - 61262..62242 (+) 981 WP_150392788.1 helix-turn-helix domain-containing protein -
  D1010_RS00280 (D1010_00280) - 62256..63869 (-) 1614 WP_152259997.1 ABC transporter ATP-binding protein -
  D1010_RS00285 (D1010_00285) - 63982..64959 (+) 978 WP_150392790.1 helix-turn-helix domain-containing protein -
  D1010_RS00290 (D1010_00290) rpsF 65112..65405 (+) 294 WP_027828812.1 30S ribosomal protein S6 -
  D1010_RS00295 (D1010_00295) ssb 65438..66025 (+) 588 WP_063516798.1 single-stranded DNA-binding protein Machinery gene
  D1010_RS00300 (D1010_00300) rpsR 66069..66308 (+) 240 WP_027828810.1 30S ribosomal protein S18 -
  D1010_RS00305 (D1010_00305) ssb 66428..66865 (+) 438 WP_027828809.1 single-stranded DNA-binding protein Machinery gene
  D1010_RS00310 (D1010_00310) - 66933..67556 (-) 624 WP_152259998.1 hypothetical protein -
  D1010_RS00315 (D1010_00315) - 67913..69145 (+) 1233 WP_152259999.1 IS110 family transposase -
  D1010_RS00320 (D1010_00320) - 69434..69634 (-) 201 WP_027828808.1 cold-shock protein -
  D1010_RS00325 (D1010_00325) - 69960..70736 (-) 777 WP_152260000.1 sulfite exporter TauE/SafE family protein -
  D1010_RS00330 (D1010_00330) - 70848..71444 (+) 597 WP_152260001.1 TMEM175 family protein -
  D1010_RS00335 (D1010_00335) - 71434..72213 (+) 780 WP_226911034.1 hypothetical protein -
  D1010_RS00340 (D1010_00340) - 72198..73223 (-) 1026 WP_146995169.1 NAD-dependent epimerase/dehydratase family protein -
  D1010_RS00345 (D1010_00345) - 73394..74764 (-) 1371 WP_152260002.1 transposase -
  D1010_RS00350 (D1010_00350) - 74901..75356 (+) 456 WP_152260003.1 MerR family transcriptional regulator -
  D1010_RS00355 (D1010_00355) - 75428..76360 (+) 933 WP_146995171.1 ATP-binding cassette domain-containing protein -
  D1010_RS00360 (D1010_00360) - 76353..77066 (+) 714 WP_152260004.1 ABC transporter permease -
  D1010_RS00365 (D1010_00365) - 77089..77802 (+) 714 WP_152260005.1 ABC transporter permease -
  D1010_RS00370 (D1010_00370) - 77948..78448 (+) 501 WP_152260006.1 EXLDI protein -
  D1010_RS00375 (D1010_00375) - 78496..79060 (+) 565 Protein_76 TMEM175 family protein -
  D1010_RS00380 (D1010_00380) - 79090..79335 (+) 246 WP_063516802.1 DUF3781 domain-containing protein -
  D1010_RS00385 (D1010_00385) - 79336..80040 (-) 705 WP_152260007.1 M23 family metallopeptidase -
  D1010_RS00390 (D1010_00390) - 80077..80730 (-) 654 WP_152260008.1 hypothetical protein -
  D1010_RS00395 (D1010_00395) - 80770..81696 (-) 927 WP_152260009.1 sensor histidine kinase KdpD -
  D1010_RS00400 (D1010_00400) - 81699..82376 (-) 678 WP_152260010.1 response regulator transcription factor -
  D1010_RS00405 (D1010_00405) - 82475..83374 (-) 900 WP_226911035.1 agmatine deiminase family protein -
  D1010_RS17960 - 83623..83790 (-) 168 WP_193312134.1 hypothetical protein -
  D1010_RS00410 (D1010_00410) - 83792..84274 (-) 483 WP_152260012.1 NUDIX hydrolase -
  D1010_RS00415 (D1010_00415) - 84348..84731 (-) 384 WP_152260013.1 hypothetical protein -
  D1010_RS00420 (D1010_00420) sdaAB 84937..85602 (+) 666 WP_152260014.1 L-serine ammonia-lyase, iron-sulfur-dependent subunit beta -
  D1010_RS00425 (D1010_00425) sdaAA 85617..86486 (+) 870 WP_152260015.1 L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha -
  D1010_RS00430 (D1010_00430) - 86483..86965 (+) 483 WP_152260016.1 kinase -
  D1010_RS00435 (D1010_00435) - 87125..89137 (+) 2013 WP_146995182.1 DHH family phosphoesterase -
  D1010_RS00440 (D1010_00440) rplI 89165..89620 (+) 456 WP_027828796.1 50S ribosomal protein L9 -
  D1010_RS00445 (D1010_00445) dnaB 89662..91071 (+) 1410 WP_027828795.1 replicative DNA helicase -
  D1010_RS00450 (D1010_00450) - 91247..92551 (+) 1305 WP_027828794.1 adenylosuccinate synthase -
  D1010_RS18870 - 92734..92802 (+) 69 Protein_93 hypothetical protein -
  D1010_RS00460 (D1010_00460) - 92861..93613 (+) 753 WP_193312135.1 ATP-binding cassette domain-containing protein -
  D1010_RS00465 (D1010_00465) - 93610..94362 (+) 753 WP_152260018.1 ABC transporter permease -
  D1010_RS00470 (D1010_00470) - 94497..94865 (+) 369 WP_027828792.1 PadR family transcriptional regulator -
  D1010_RS00475 (D1010_00475) - 94837..95670 (+) 834 WP_152260019.1 hypothetical protein -
  D1010_RS00480 (D1010_00480) - 95810..96508 (+) 699 WP_152260020.1 hypothetical protein -
  D1010_RS00485 (D1010_00485) - 96505..100419 (-) 3915 WP_152260021.1 lectin-like domain-containing protein -
  D1010_RS00490 (D1010_00490) - 100641..101177 (+) 537 WP_063516101.1 TetR/AcrR family transcriptional regulator -
  D1010_RS00495 (D1010_00495) - 101177..102235 (+) 1059 WP_152260022.1 ABC transporter permease -
  D1010_RS00500 (D1010_00500) - 102250..102933 (+) 684 WP_027828787.1 ABC transporter ATP-binding protein -
  D1010_RS00505 (D1010_00505) - 103068..103571 (+) 504 WP_152260023.1 hypothetical protein -
  D1010_RS00510 (D1010_00510) - 103514..103858 (+) 345 WP_264371664.1 CPBP family intramembrane glutamic endopeptidase -
  D1010_RS00515 (D1010_00515) - 103909..104532 (+) 624 WP_152260025.1 SAP domain-containing protein -
  D1010_RS00520 (D1010_00520) - 104529..104858 (-) 330 WP_152260026.1 MazG nucleotide pyrophosphohydrolase domain-containing protein -
  D1010_RS00525 (D1010_00525) - 104935..105120 (-) 186 WP_152260027.1 hypothetical protein -
  D1010_RS00530 (D1010_00530) - 105304..106674 (+) 1371 WP_152260028.1 transposase -
  D1010_RS00535 (D1010_00535) - 106746..107162 (-) 417 WP_152260029.1 ABC-2 transporter permease -
  D1010_RS00540 (D1010_00540) - 107140..107880 (-) 741 WP_152260030.1 ABC transporter ATP-binding protein -
  D1010_RS00545 (D1010_00545) - 107870..108331 (-) 462 WP_152260031.1 helix-turn-helix domain-containing protein -
  D1010_RS00550 (D1010_00550) - 108527..108739 (+) 213 WP_027828781.1 helix-turn-helix transcriptional regulator -
  D1010_RS00555 (D1010_00555) - 108736..109242 (+) 507 WP_152260032.1 DUF3278 domain-containing protein -
  D1010_RS00560 (D1010_00560) - 109396..110157 (+) 762 WP_152260033.1 CPBP family intramembrane glutamic endopeptidase -
  D1010_RS00565 (D1010_00565) - 110180..110869 (+) 690 WP_152260034.1 CPBP family intramembrane glutamic endopeptidase -
  D1010_RS00570 (D1010_00570) - 110942..111172 (-) 231 WP_063516456.1 CsbD family protein -
  D1010_RS00575 (D1010_00575) - 111207..111380 (-) 174 WP_082231185.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -

Sequence


Protein


Download         Length: 145 a.a.        Molecular weight: 15786.64 Da        Isoelectric Point: 4.9527

>NTDB_id=392299 D1010_RS00305 WP_027828809.1 66428..66865(+) (ssb) [Schleiferilactobacillus harbinensis strain M1]
MINRVTLVGRLVRDVELKYTPGGTARAWFNLAVTRNFKNANGERDSDFIECTVWGKSAESFVNFTCKGSLVGIEGQIRTR
SYEKDGQKVYASAVSVDSFALLESKAITDARRAGQPVTTAGKDDNVFVAAGNGENIDIADDDLPF

Nucleotide


Download         Length: 438 bp        

>NTDB_id=392299 D1010_RS00305 WP_027828809.1 66428..66865(+) (ssb) [Schleiferilactobacillus harbinensis strain M1]
ATGATTAATCGGGTAACGCTGGTGGGCCGCTTGGTGCGGGATGTGGAGTTAAAGTATACGCCGGGCGGAACTGCGCGGGC
ATGGTTCAATCTGGCAGTGACGCGTAATTTTAAGAACGCCAACGGTGAACGGGATTCTGATTTTATCGAATGTACGGTAT
GGGGCAAGTCGGCGGAATCATTTGTCAACTTCACCTGCAAGGGTTCTCTGGTGGGAATTGAGGGCCAAATCCGGACGCGC
AGCTATGAGAAAGATGGTCAGAAAGTTTACGCCTCGGCAGTCTCGGTGGATAGTTTTGCCCTCCTGGAATCAAAGGCCAT
AACTGACGCCCGGCGTGCTGGCCAGCCAGTAACAACTGCTGGAAAAGACGACAACGTCTTCGTCGCCGCGGGCAATGGCG
AAAACATCGACATCGCCGATGACGACCTGCCATTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0R1XHW9

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

48.235

100

0.566

  ssbA Bacillus subtilis subsp. subtilis str. 168

41.279

100

0.49

  ssbB Streptococcus sobrinus strain NIDR 6715-7

40

100

0.4

  ssbB/cilA Streptococcus mitis NCTC 12261

37.931

100

0.379

  ssbB/cilA Streptococcus pneumoniae TIGR4

37.241

100

0.372

  ssbB/cilA Streptococcus mitis SK321

37.241

100

0.372

  ssbB/cilA Streptococcus pneumoniae Rx1

36.552

100

0.366

  ssbB/cilA Streptococcus pneumoniae D39

36.552

100

0.366

  ssbB/cilA Streptococcus pneumoniae R6

36.552

100

0.366


Multiple sequence alignment