Detailed information NTG0392298

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: HO399_RS04160
• Genome accession: NZ_CP053599
• Coordinates: 891765..892748 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain NEBExpress
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 886765..897748

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HO399_RS04145 (HO399_04145)	dppA	887908..889515 (+)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
HO399_RS21920	-	889549..889659 (-)	111	Protein_805	hypothetical protein	-
HO399_RS04150 (HO399_04150)	dppB	889823..890842 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
HO399_RS04155 (HO399_04155)	dppC	890852..891754 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
HO399_RS04160 (HO399_04160)	amiE	891765..892748 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
HO399_RS04165 (HO399_04165)	dppF	892745..893749 (+)	1005	WP_000107012.1	dipeptide ABC transporter ATP-binding subunit DppF	-
HO399_RS04170 (HO399_04170)	yhjV	893779..895050 (-)	1272	WP_001295225.1	aromatic amino acid transport family protein	-
HO399_RS04175 (HO399_04175)	-	895526..895633 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
HO399_RS04180 (HO399_04180)	-	896009..896116 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
HO399_RS04185 (HO399_04185)	ldrD	896492..896599 (+)	108	WP_000141634.1	type I toxin-antitoxin system toxic polypeptide LdrD	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=392298 HO399_RS04160 WP_001196486.1 891765..892748(+) (amiE) [Escherichia coli strain NEBExpress]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=392298 HO399_RS04160 WP_001196486.1 891765..892748(+) (amiE) [Escherichia coli strain NEBExpress]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404