Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GBN67_RS00610 Genome accession   NZ_CP045051
Coordinates   127891..129975 (+) Length   694 a.a.
NCBI ID   WP_234963357.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ312     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 122891..134975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN67_RS00590 (GBN67_00590) pilM 124911..125969 (+) 1059 WP_058870420.1 pilus assembly protein PilM Machinery gene
  GBN67_RS00595 (GBN67_00595) pilN 125969..126625 (+) 657 WP_234963356.1 PilN domain-containing protein Machinery gene
  GBN67_RS00600 (GBN67_00600) pilO 126622..127344 (+) 723 WP_004691497.1 type 4a pilus biogenesis protein PilO Machinery gene
  GBN67_RS00605 (GBN67_00605) pilP 127344..127871 (+) 528 WP_004691496.1 pilus assembly protein PilP Machinery gene
  GBN67_RS00610 (GBN67_00610) pilQ 127891..129975 (+) 2085 WP_234963357.1 type IV pilus secretin PilQ family protein Machinery gene
  GBN67_RS00615 (GBN67_00615) - 130064..130546 (+) 483 WP_207778853.1 shikimate kinase -
  GBN67_RS00620 (GBN67_00620) aroB 130577..131662 (+) 1086 WP_234954450.1 3-dehydroquinate synthase -
  GBN67_RS00625 (GBN67_00625) - 131670..132509 (+) 840 WP_234954448.1 hypothetical protein -

Sequence

Protein


Download         Length: 694 a.a.        Molecular weight: 75450.62 Da        Isoelectric Point: 5.1564

>NTDB_id=391519 GBN67_RS00610 WP_234963357.1 127891..129975(+) (pilQ) [Acinetobacter johnsonii strain AJ312]
MKNSAKDFIFGDGNTMNHMFRQFSMGAIAMAVMQAASAQVSITNIVPMQIPGQGTEIRVMFNGLPPQPQAYQLEQPSRLV
LDFDKAQQSLKQGSIAVATAEASTVDVTSDAQRSRLTVNLADAGAFTTRVEGNTFILKINSANPALATPVAATRSEQGIS
NIGFQRGSDGEGQVVIDLKGSNTPVDVKQQGSKIVVRVLGNKIPVHLSRRLNVNDFATPVSTIDAQNDGSNGLITIQSNG
SFEYMAYQAEDKLTISLKRPEDKNPMKPKAPSAYTGKKISLDFQDIEVRRVLQLLADFTGINMVASDTVQGNITLRLKDV
PWDQALDIIMKTKNLDKRRNGNVIWIAPVAELIKSEEDEAKAIAQSIKLSPIQTDYIQLSYAKSADILKLLEDSRDSKGS
ESNRTAGSDSLALESLLSARGSAVSDSRTNTLIINDTQQNIDKIRRMIDLLDVAVKQVMVEARIVTASTDFSRELGIKWS
AKKEAGNNSPTWGGDFQPSFDLGVDLATSTAGTFNFGLLKISDYMLNLELTALQADGHGEVLSAPKVLTGDKQKAYILRG
TEIPYQTWSATEGLKTEFRAANLKLEVTPSITPDGKVQMALMVEKDSIGDITNDGIAIDTSELQTNVLVADGETVVLGGI
FDDVKRNDYQKVPFLGDLPVVGNLFKNNTKTTMQTELLIFVTPRIVNDSVSRNH

Nucleotide


Download         Length: 2085 bp        

>NTDB_id=391519 GBN67_RS00610 WP_234963357.1 127891..129975(+) (pilQ) [Acinetobacter johnsonii strain AJ312]
ATGAAAAATAGTGCTAAAGATTTTATATTTGGGGATGGCAATACGATGAACCATATGTTCCGTCAGTTTTCAATGGGTGC
AATTGCAATGGCGGTGATGCAGGCTGCAAGTGCGCAAGTCAGTATTACGAATATTGTGCCGATGCAAATTCCGGGACAGG
GAACAGAAATTCGTGTCATGTTCAACGGTTTGCCACCACAGCCGCAAGCCTATCAGTTGGAACAGCCATCACGTTTGGTT
TTGGATTTTGATAAAGCACAGCAGAGCCTAAAACAAGGCAGTATTGCGGTAGCAACGGCTGAAGCAAGTACAGTAGATGT
AACTTCAGATGCGCAGCGTTCACGTTTAACTGTAAATTTGGCTGATGCGGGTGCATTTACTACACGTGTTGAAGGTAATA
CCTTTATTCTGAAAATTAACTCAGCGAACCCGGCGCTAGCAACACCTGTTGCTGCGACGCGTTCAGAGCAAGGTATTTCA
AATATTGGCTTCCAGCGTGGTTCAGACGGTGAAGGCCAAGTTGTGATTGATTTAAAAGGTAGCAATACCCCTGTCGATGT
GAAACAACAAGGCAGTAAAATTGTGGTTCGTGTTTTGGGAAATAAAATTCCTGTCCATTTATCACGTCGTCTAAATGTGA
ATGATTTCGCAACACCAGTCTCGACCATTGATGCACAAAACGATGGTAGTAACGGCCTGATTACGATTCAATCGAACGGC
AGTTTTGAATATATGGCTTATCAGGCAGAAGATAAGCTCACCATTAGTTTAAAGCGTCCTGAAGATAAAAATCCAATGAA
GCCTAAAGCACCAAGTGCTTATACGGGCAAAAAGATTTCTTTAGATTTTCAAGATATTGAAGTGCGACGTGTATTACAGC
TCTTAGCTGATTTTACAGGCATTAACATGGTGGCTTCTGACACTGTACAAGGGAATATTACCTTGCGCCTTAAAGATGTG
CCATGGGATCAAGCGCTTGATATTATTATGAAAACCAAGAATTTGGATAAGCGACGCAATGGCAATGTGATTTGGATTGC
ACCTGTAGCTGAACTGATCAAGTCTGAGGAAGATGAAGCCAAAGCGATTGCGCAAAGTATTAAGCTTTCACCAATACAAA
CCGATTACATTCAATTGAGTTATGCTAAATCGGCGGATATACTGAAGTTGTTGGAAGACTCTCGTGATAGCAAAGGAAGT
GAGTCAAATCGTACAGCGGGCTCAGACTCCTTGGCTTTGGAAAGTTTGTTGAGTGCTCGTGGTAGTGCAGTTTCTGACTC
GCGTACCAATACGCTAATCATTAATGATACGCAGCAAAATATTGACAAAATCCGACGTATGATCGACTTACTGGATGTCG
CAGTGAAACAGGTGATGGTCGAAGCACGTATTGTGACAGCGTCAACAGACTTTTCTCGTGAATTGGGGATTAAGTGGAGT
GCGAAGAAAGAGGCGGGAAATAATAGCCCAACTTGGGGTGGGGATTTTCAACCAAGTTTTGATTTAGGTGTGGATTTGGC
GACCTCGACAGCAGGAACATTTAACTTTGGTTTATTGAAAATTTCAGACTATATGTTAAACCTTGAACTAACCGCACTTC
AAGCAGATGGACATGGTGAAGTCTTGTCGGCGCCTAAAGTACTTACTGGCGATAAACAGAAAGCCTATATCTTACGTGGT
ACAGAGATTCCTTATCAAACATGGAGTGCGACGGAAGGCTTAAAAACCGAGTTCCGTGCGGCAAACTTGAAGTTAGAAGT
GACGCCAAGCATTACCCCGGATGGTAAAGTTCAGATGGCACTGATGGTTGAGAAAGACTCGATTGGTGACATTACCAATG
ATGGTATTGCAATTGACACCAGTGAATTACAAACCAATGTGTTGGTTGCGGATGGTGAGACTGTCGTACTGGGTGGTATT
TTTGATGATGTGAAACGTAATGACTATCAAAAAGTGCCATTTTTAGGTGATTTACCCGTAGTTGGTAATTTATTTAAGAA
TAATACCAAAACGACGATGCAAACTGAGTTGTTGATTTTTGTGACACCACGAATTGTTAATGACAGTGTTTCAAGAAATC
ATTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(305-348)

AMIN domain (AMIN)

(55-135)

Bacterial type II and III secretion system protein (Secretin)

(532-686)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Acinetobacter baumannii D1279779

63.939

100

0.664
  pilQ Acinetobacter baumannii strain A118

63.8

100

0.663
  comQ Acinetobacter baylyi ADP1

62.64

100

0.643
  pilQ Pseudomonas aeruginosa PAK

39.595

99.712

0.395
  pilQ Legionella pneumophila strain Lp02

37.881

99.28

0.376
  pilQ Legionella pneumophila strain ERS1305867

38.166

97.406

0.372

Multiple sequence alignment