Detailed information NTG0391515

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: GBN67_RS00590
• Genome accession: NZ_CP045051
• Coordinates: 124911..125969 (+)
• Length: 352 a.a.
• NCBI ID: WP_058870420.1
• Uniprot ID: A0A345B4L2
• Organism: Acinetobacter johnsonii strain AJ312
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 119911..130969

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GBN67_RS00575 (GBN67_00575)	mraY	120190..121308 (+)	1119	WP_058951625.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-
GBN67_RS00580 (GBN67_00580)	-	121378..122184 (+)	807	WP_234963354.1	putative RNA methyltransferase	-
GBN67_RS00585 (GBN67_00585)	ponA	122216..124744 (-)	2529	WP_234963355.1	penicillin-binding protein PBP1a	-
GBN67_RS00590 (GBN67_00590)	pilM	124911..125969 (+)	1059	WP_058870420.1	pilus assembly protein PilM	Machinery gene
GBN67_RS00595 (GBN67_00595)	pilN	125969..126625 (+)	657	WP_234963356.1	PilN domain-containing protein	Machinery gene
GBN67_RS00600 (GBN67_00600)	pilO	126622..127344 (+)	723	WP_004691497.1	type 4a pilus biogenesis protein PilO	Machinery gene
GBN67_RS00605 (GBN67_00605)	pilP	127344..127871 (+)	528	WP_004691496.1	pilus assembly protein PilP	Machinery gene
GBN67_RS00610 (GBN67_00610)	pilQ	127891..129975 (+)	2085	WP_234963357.1	type IV pilus secretin PilQ family protein	Machinery gene
GBN67_RS00615 (GBN67_00615)	-	130064..130546 (+)	483	WP_207778853.1	shikimate kinase	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38953.50 Da
• Isoelectric Point: 4.4181

>NTDB_id=391515 GBN67_RS00590 WP_058870420.1 124911..125969(+) (pilM) [Acinetobacter johnsonii strain AJ312]
MLRLYRKPSKGLIGVDISSTSVKVLELSVKNNRYWVESYALVPLPEGSVVEKNILNPEAVGDALERAVNLANVQSTDVAL
AVPTSMVITKIIEMDADMNDDEREIQIRDDAEQYIPFPLDEASLDFEVLPDRLANPNRVNVLLVATRIENVEARSEALEL
GGLTPKIADVESFAIENAFKVFSDTLPMGVNTVGILDIGHTMTTLSVMQNNKVIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKSRTLPDDYDIEVLEPFLEAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=391515 GBN67_RS00590 WP_058870420.1 124911..125969(+) (pilM) [Acinetobacter johnsonii strain AJ312]
GTGCTCAGGTTATATCGTAAGCCAAGTAAGGGGTTAATAGGAGTAGATATTAGTTCGACTTCTGTTAAGGTATTGGAACT
TTCTGTTAAAAACAATCGGTATTGGGTGGAGAGCTATGCACTAGTCCCATTACCAGAAGGAAGTGTGGTCGAAAAAAATA
TATTGAATCCAGAAGCGGTTGGTGATGCCCTTGAGCGTGCGGTAAATTTGGCCAATGTTCAATCGACAGACGTAGCACTG
GCAGTTCCGACGTCCATGGTGATTACTAAAATCATTGAAATGGATGCCGATATGAACGATGACGAACGTGAAATTCAGAT
TCGTGATGATGCTGAGCAATATATTCCATTCCCATTAGATGAAGCGAGTTTGGACTTTGAAGTCCTTCCAGACCGTTTAG
CCAATCCCAATCGTGTCAATGTACTCCTTGTTGCAACCCGCATTGAAAATGTAGAGGCGCGTTCCGAAGCATTGGAGTTA
GGTGGTTTAACGCCTAAGATTGCCGATGTTGAAAGTTTCGCCATTGAAAATGCATTCAAAGTCTTTTCAGATACGCTTCC
AATGGGTGTAAACACCGTTGGGATTCTGGACATCGGTCATACCATGACCACCTTATCTGTCATGCAGAATAATAAAGTCA
TTTATACCCGTGAGCAGGTGTTCGGTGGTAAACAACTGACTCAAGAAATTCAAAACCGTTATGGTTTGTCTTTTGAAGAA
GCTGGACGTGCCAAAAAAAGCCGTACCTTGCCTGACGACTATGATATTGAAGTACTTGAACCTTTTCTTGAAGCTGTGGT
GCAACAAGCAGCACGTTCTTTGCAGTTCTTCTTTTCGTCTTCTCAATTCAATGAGATCGACCATATTTTATTGGCAGGGG
GGAATGCCAATATTCCAGGACTGGCGAAGTTATTACAACAAAAATTAGGTTATCGGGTCACTATTGCCAACCCATTTTTA
CAGATGGGCTTTTCTCCACAAATCGACATTAAAAAAATTGAAAATGATGCTTCATCACTCATGGTGGCATGTGGATTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A345B4L2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	85.227	100	0.852
comM	Acinetobacter nosocomialis M2	85.227	100	0.852
comM	Acinetobacter baylyi ADP1	80.398	100	0.804
pilM	Legionella pneumophila strain ERS1305867	40.96	100	0.412