Detailed information NTG0391517

Bioinformatically predicted

Overview

• Name: pilO
• Type: Machinery gene
• Locus tag: GBN67_RS00600
• Genome accession: NZ_CP045051
• Coordinates: 126622..127344 (+)
• Length: 240 a.a.
• NCBI ID: WP_004691497.1
• Uniprot ID: A0A380U8X3
• Organism: Acinetobacter johnsonii strain AJ312
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 121622..132344

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GBN67_RS00585 (GBN67_00585)	ponA	122216..124744 (-)	2529	WP_234963355.1	penicillin-binding protein PBP1a	-
GBN67_RS00590 (GBN67_00590)	pilM	124911..125969 (+)	1059	WP_058870420.1	pilus assembly protein PilM	Machinery gene
GBN67_RS00595 (GBN67_00595)	pilN	125969..126625 (+)	657	WP_234963356.1	PilN domain-containing protein	Machinery gene
GBN67_RS00600 (GBN67_00600)	pilO	126622..127344 (+)	723	WP_004691497.1	type 4a pilus biogenesis protein PilO	Machinery gene
GBN67_RS00605 (GBN67_00605)	pilP	127344..127871 (+)	528	WP_004691496.1	pilus assembly protein PilP	Machinery gene
GBN67_RS00610 (GBN67_00610)	pilQ	127891..129975 (+)	2085	WP_234963357.1	type IV pilus secretin PilQ family protein	Machinery gene
GBN67_RS00615 (GBN67_00615)	-	130064..130546 (+)	483	WP_207778853.1	shikimate kinase	-
GBN67_RS00620 (GBN67_00620)	aroB	130577..131662 (+)	1086	WP_234954450.1	3-dehydroquinate synthase	-

Sequence

Protein

• Length: 240 a.a.
• Molecular weight: 27004.43 Da
• Isoelectric Point: 4.9937

>NTDB_id=391517 GBN67_RS00600 WP_004691497.1 126622..127344(+) (pilO) [Acinetobacter johnsonii strain AJ312]
MSRDEFEELTSENHAVTKKKMTVDKFFQQFNTLDPNNYGSWPFSVKLTCWIFIILVIGALGYFVVIKPKLDAISNAHAQE
QNLLNEFREKDSKLRNLQQYQAQLQEMEANFNQQLEQLPKETEIPGLVEDINVSGVNSGLKFKNIRLEPEIRQEFFIEQP
ISIEATGDYHSFGSFVSGIAALPRIVTLHDFDISASSNNEKKTDIPQISYSVKAKTYRYVGATAAASVENTAKADAGGAQ

Nucleotide

• Length: 723 bp

>NTDB_id=391517 GBN67_RS00600 WP_004691497.1 126622..127344(+) (pilO) [Acinetobacter johnsonii strain AJ312]
ATGAGTCGCGATGAATTTGAAGAATTAACATCAGAAAACCATGCTGTAACCAAGAAAAAAATGACGGTTGATAAGTTTTT
TCAACAGTTTAATACCTTAGATCCAAACAATTACGGTAGTTGGCCCTTTTCAGTCAAATTGACTTGTTGGATCTTTATCA
TTTTAGTAATTGGGGCATTGGGTTATTTTGTCGTGATTAAACCGAAGCTTGATGCGATTTCAAATGCACATGCACAAGAA
CAAAATTTACTGAATGAGTTTCGTGAAAAAGATTCAAAATTACGTAATTTGCAGCAATATCAAGCGCAATTGCAAGAAAT
GGAAGCCAATTTTAATCAGCAGTTAGAACAGCTGCCGAAAGAAACTGAAATTCCTGGTTTGGTTGAAGATATCAACGTGA
GCGGCGTAAATTCTGGATTAAAATTTAAAAATATTCGTTTAGAGCCAGAAATTCGCCAAGAGTTCTTTATCGAACAGCCG
ATTTCCATTGAAGCGACGGGTGATTATCATTCTTTTGGTTCATTTGTCAGTGGAATTGCAGCTTTACCGCGTATTGTGAC
CTTGCATGACTTCGATATCTCAGCATCAAGTAATAATGAGAAAAAGACGGATATTCCGCAAATTTCATACAGTGTAAAGG
CTAAAACTTACCGCTATGTCGGGGCGACAGCTGCTGCAAGTGTGGAAAATACAGCTAAAGCAGATGCAGGAGGTGCTCAG
TAA

Domains

Predicted by InterproScan.

• Pilus assembly protein, PilO (PilO) (74-220)

3D structure

• AlphaFold DB: A0A380U8X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilO	Acinetobacter baumannii D1279779	72.385	99.583	0.721
comO	Acinetobacter baylyi ADP1	67.755	100	0.692