Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F8A90_RS03700 Genome accession   NZ_CP044522
Coordinates   863776..865512 (-) Length   578 a.a.
NCBI ID   WP_200019034.1    Uniprot ID   -
Organism   Cobetia sp. cqz5-12     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 858776..870512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F8A90_RS03675 (F8A90_03675) - 859407..860360 (+) 954 WP_200019026.1 Nudix family hydrolase -
  F8A90_RS03680 (F8A90_03680) yacG 860524..860769 (-) 246 WP_043332481.1 DNA gyrase inhibitor YacG -
  F8A90_RS03685 (F8A90_03685) coaE 860762..861412 (-) 651 WP_200019028.1 dephospho-CoA kinase -
  F8A90_RS03690 (F8A90_03690) pilD 861409..862269 (-) 861 WP_200019030.1 A24 family peptidase Machinery gene
  F8A90_RS03695 (F8A90_03695) pilC 862360..863598 (-) 1239 WP_200019032.1 type II secretion system F family protein Machinery gene
  F8A90_RS03700 (F8A90_03700) pilB 863776..865512 (-) 1737 WP_200019034.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F8A90_RS03705 (F8A90_03705) comP 866086..866523 (+) 438 WP_200019036.1 pilin Machinery gene
  F8A90_RS03710 (F8A90_03710) - 866594..868360 (+) 1767 WP_200019038.1 hypothetical protein -
  F8A90_RS03715 (F8A90_03715) - 868411..868713 (-) 303 WP_043332474.1 hypothetical protein -
  F8A90_RS03720 (F8A90_03720) - 868884..870182 (-) 1299 WP_200019040.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63543.63 Da        Isoelectric Point: 4.8371

>NTDB_id=390819 F8A90_RS03700 WP_200019034.1 863776..865512(-) (pilB) [Cobetia sp. cqz5-12]
MPQSEHMLKGFAARLVDSGLLEPQAAQLAEQEANEAGESLLLHIIAHGLVTARDATLAAAWEYGLPYVDLDALRIDSLPS
IDELPEKVLRKLGVLPLYRREHRLTVGVPSPSTLTHLDELQFATGLSIESVLVAADQLTPALEQYLEAGEESAMDMLSED
GEALDGLDFEGEEDNNNDLAITANSDDAPVVRFVHKVLLDAIKRGASDIHFEPYESTYRIRFRIDGMLVENTRPPFNMRN
RISARLKVMSRMDISERRLPQDGAIKLRLSKSRSIDFRVNTLPTVYGEKLVLRILDPSSAKMGIEALGFEPEQRAMFEKT
LNQPQGMILVTGPTGSGKTVTLYTGLNILNTPERNISTAEDPVELKVEGVNQVNVLPKIGLDFASALRAFLRQDPDVVMV
GEIRDKETAEIAVKAAQTGHLVLSTLHTNSAPETLTRLSNMGIAPFNIASSVSLIIAQRLARRLCTHCRQPTEIPPEAMA
SMGFSEDNGFSAAELEAATIFRPVGCSQCTLGYKGRVGIYEVMPISDEMSRLIMREGTAIELADQARKEGLSDLRRSGLR
KVMSGMTSLEEVNRITKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=390819 F8A90_RS03700 WP_200019034.1 863776..865512(-) (pilB) [Cobetia sp. cqz5-12]
ATGCCCCAGTCAGAACACATGCTAAAAGGATTTGCCGCCCGACTGGTCGACTCTGGCCTGCTCGAACCACAGGCAGCACA
GCTGGCTGAACAGGAAGCCAATGAGGCCGGGGAAAGCCTGTTGCTGCACATCATTGCGCATGGTCTGGTGACAGCACGTG
ACGCGACATTGGCGGCCGCCTGGGAATATGGCCTGCCGTATGTGGATCTCGATGCCCTTCGTATCGACTCGCTGCCTTCC
ATTGACGAGCTGCCCGAGAAGGTCTTGCGCAAGCTGGGTGTACTGCCACTCTATCGCCGTGAGCATCGGCTGACCGTCGG
CGTGCCCTCCCCATCAACACTTACGCATTTAGATGAGCTGCAATTTGCCACTGGCTTGTCGATCGAGAGTGTCCTGGTCG
CCGCCGACCAGCTGACGCCAGCATTGGAGCAGTACCTGGAAGCCGGCGAAGAAAGCGCCATGGACATGCTGAGCGAAGAT
GGCGAAGCACTCGATGGGCTGGATTTTGAAGGCGAAGAAGATAACAACAACGATCTAGCCATTACAGCCAACAGTGATGA
TGCGCCAGTGGTGCGCTTCGTTCACAAGGTACTGCTGGATGCCATCAAGCGTGGTGCCTCGGATATCCACTTCGAGCCCT
ACGAATCCACCTATCGCATTCGTTTTCGTATCGATGGCATGTTGGTTGAGAATACCCGTCCACCCTTCAATATGCGTAAC
CGGATCTCGGCACGCCTGAAGGTCATGTCGCGCATGGATATTTCTGAGCGCCGTTTACCGCAGGACGGCGCGATCAAGCT
GCGCCTGTCCAAGTCCCGTTCCATCGATTTCCGCGTCAATACACTACCCACCGTGTATGGCGAGAAGCTGGTACTGCGTA
TCCTGGACCCCAGCTCGGCCAAGATGGGCATCGAGGCGCTGGGCTTCGAACCCGAACAGCGCGCCATGTTCGAAAAGACG
CTGAATCAGCCCCAGGGCATGATTCTGGTCACTGGGCCTACCGGTAGCGGCAAGACCGTGACGCTCTACACCGGCTTGAA
TATCCTCAATACACCGGAGCGCAATATCTCGACGGCGGAAGATCCGGTCGAACTCAAGGTAGAAGGCGTCAACCAGGTCA
ACGTGCTACCCAAGATCGGACTGGACTTCGCCTCGGCCCTGCGCGCCTTCCTGCGCCAGGATCCCGATGTAGTGATGGTG
GGGGAAATTCGAGACAAGGAAACCGCCGAGATTGCTGTCAAGGCTGCACAGACCGGCCACCTGGTGCTTTCTACCCTGCA
CACCAACTCAGCACCCGAGACACTGACGCGTCTGAGCAACATGGGGATTGCCCCTTTCAACATCGCGAGCTCCGTCTCGT
TGATCATCGCTCAGCGCCTTGCGCGTCGCCTGTGCACCCACTGCCGCCAGCCCACGGAAATTCCGCCCGAGGCGATGGCC
TCGATGGGCTTCAGTGAAGACAATGGCTTCAGTGCAGCAGAACTCGAGGCAGCGACCATATTCCGCCCCGTCGGTTGCAG
CCAGTGCACACTGGGTTACAAGGGGCGAGTCGGCATTTATGAAGTCATGCCGATCTCGGATGAGATGAGCCGCTTGATCA
TGCGTGAGGGCACCGCCATCGAGCTGGCAGACCAGGCGCGCAAGGAAGGGCTTTCTGACCTGAGGCGTAGCGGCTTGCGC
AAGGTGATGTCGGGCATGACCTCGCTGGAAGAAGTCAATCGTATCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

52.273

98.962

0.517
  pilB Acinetobacter baumannii D1279779

52.197

98.443

0.514
  pilB Legionella pneumophila strain ERS1305867

51.304

99.481

0.51
  pilB Vibrio parahaemolyticus RIMD 2210633

47.652

99.481

0.474
  pilB Vibrio cholerae strain A1552

46.655

100

0.471
  pilB Vibrio campbellii strain DS40M4

47.038

99.308

0.467
  pilF Neisseria gonorrhoeae MS11

47.662

96.194

0.458
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.715

99.654

0.386
  pilB/pilB1 Synechocystis sp. PCC 6803

42.015

91.003

0.382
  pilF Thermus thermophilus HB27

38.584

97.751

0.377

Multiple sequence alignment