Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   NLA_RS02055 Genome accession   NC_014752
Coordinates   361761..362993 (-) Length   410 a.a.
NCBI ID   WP_003710974.1    Uniprot ID   E4ZBA3
Organism   Neisseria lactamica 020-06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 356761..367993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLA_RS02020 (NLA_3840) - 357145..357591 (+) 447 WP_013448429.1 DUF441 domain-containing protein -
  NLA_RS02030 (NLA_3850) - 357772..357978 (+) 207 WP_003714007.1 hypothetical protein -
  NLA_RS02035 (NLA_3860) pilF 358226..359902 (+) 1677 WP_003710978.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NLA_RS02040 (NLA_3870) - 359987..360196 (-) 210 WP_013448430.1 DNA gyrase inhibitor YacG -
  NLA_RS02045 (NLA_3880) coaE 360189..360821 (-) 633 WP_013448431.1 dephospho-CoA kinase -
  NLA_RS02050 (NLA_3890) pilD 360823..361683 (-) 861 WP_013448432.1 prepilin peptidase Machinery gene
  NLA_RS02055 (NLA_3900) pilG 361761..362993 (-) 1233 WP_003710974.1 type 4 pilus assembly protein PilG Machinery gene
  NLA_RS02060 (NLA_3910) pgi 363347..364990 (+) 1644 WP_042508132.1 glucose-6-phosphate isomerase -
  NLA_RS02065 - 364960..365190 (+) 231 WP_036475185.1 hypothetical protein -
  NLA_RS02070 (NLA_3920) dapD 365200..366021 (+) 822 WP_013448434.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  NLA_RS02075 (NLA_3930) fabI 366176..366961 (+) 786 WP_002223367.1 enoyl-ACP reductase FabI -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 45246.23 Da        Isoelectric Point: 10.0085

>NTDB_id=39054 NLA_RS02055 WP_003710974.1 361761..362993(-) (pilG) [Neisseria lactamica 020-06]
MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARKKLAKRGIRPLQVTRVKTSSKRKITQEDITVFTRQLS
TMIKAGLPLMQAFEIVARGHGNPSMTEMLMEIRSNVEQGSTLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAVY
KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTVMDMSDFFVSYGWMVLIALGFAI
YGFLKLKARSIKIQRRMDALLLRMPIFGDIVRKGTIARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRV
IQGLSMTSGMRATELFPNMMVQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIGTLLVAMYL
PLFNLGNVVG

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=39054 NLA_RS02055 WP_003710974.1 361761..362993(-) (pilG) [Neisseria lactamica 020-06]
ATGGCTAAAAATGGGGGATTTTCTTTGTTCGCAAAGAAAGAAAAACGCTTTATCTTTGAAGGCAGGCATTCCGCCTCCGA
CAAACTGGTCAACGGCGAAGTATCTGCGTTTACCGAAGAAGAGGCACGCAAAAAACTCGCCAAACGCGGCATCCGCCCGC
TGCAGGTTACCCGTGTGAAAACAAGCTCCAAGCGCAAAATCACACAAGAAGACATCACCGTCTTCACCCGCCAGCTGTCC
ACGATGATTAAAGCGGGCCTGCCGCTGATGCAGGCATTTGAAATCGTGGCGCGCGGACACGGCAACCCGTCTATGACGGA
AATGCTGATGGAAATCCGCAGCAATGTGGAACAGGGCAGCACGTTGAGCCGCGCATTCTCGAACCACCCTAAATATTTCG
ACCGCTTCTACTGCAACCTGGTTGCGGCGGGCGAAACGGGCGGCGTGTTGGAAAGCCTGTTGGACAAATTGGCAGTCTAT
AAAGAAAAAACCCAGGCCATCCGCAAAAAGGTAAAAACCGCGCTGACCTATCCGGTATCCGTCATCGCCGTCGCCATCGG
TTTGGTATTCGTGATGATGATTTTCGTACTGCCCGCCTTTAAAGAGGTTTACGCCAATATGGGCGCGGAGCTTCCCGCAC
TGACCCAAACAGTGATGGATATGTCCGACTTTTTCGTCTCATACGGCTGGATGGTGCTGATCGCACTCGGCTTTGCCATA
TACGGCTTCCTCAAATTGAAGGCGCGTTCGATTAAAATCCAACGGCGTATGGATGCCCTGCTGCTGCGTATGCCGATTTT
CGGAGACATCGTCCGTAAAGGGACGATTGCCCGCTGGGGCAGGACGACGGCGACACTGATTGCGGCAGGCGTGCCTTTGG
TCGATGTATTGGACTCCACTGCCGGCGCGGCGGGCAATTTAATCTATGAAGAAGCCACCCGGGAAATCCGTACGCGCGTC
ATCCAAGGCCTGTCTATGACTTCGGGGATGCGTGCGACGGAACTGTTCCCCAATATGATGGTGCAGATGTCCTCCATCGG
CGAGGAATCGGGTTCTTTGGACGATATGCTCAACAAAGCCGCCGAATTTTATGAAGACGAGGTGGACAATGCGGTCGGCA
GGCTGTCCGCTATGATGGAGCCGATCATTATCGTGATTTTGGGCTTGGTCATCGGTACGCTTCTGGTCGCTATGTATCTG
CCGCTGTTCAACTTGGGCAACGTGGTCGGCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(281-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4ZBA3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

97.8

99.756

0.976
  pilG Neisseria meningitidis 44/76-A

97.066

99.756

0.968
  pilC Acinetobacter baumannii D1279779

47.487

97.073

0.461
  pilC Acinetobacter baylyi ADP1

46.115

97.317

0.449
  pilC Legionella pneumophila strain ERS1305867

45.34

96.829

0.439
  pilC Pseudomonas stutzeri DSM 10701

44.527

98.049

0.437
  pilC Vibrio cholerae strain A1552

40.943

98.293

0.402
  pilC Vibrio campbellii strain DS40M4

39.066

99.268

0.388

Multiple sequence alignment