Detailed information NTG0039053

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NLA_RS02050
• Genome accession: NC_014752
• Coordinates: 360823..361683 (-)
• Length: 286 a.a.
• NCBI ID: WP_013448432.1
• Uniprot ID: E4ZBA2
• Organism: Neisseria lactamica 020-06
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 355823..366683

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NLA_RS02015 (NLA_3830)	-	356166..357140 (+)	975	WP_003710984.1	polyprenyl synthetase family protein	-
NLA_RS02020 (NLA_3840)	-	357145..357591 (+)	447	WP_013448429.1	DUF441 domain-containing protein	-
NLA_RS02030 (NLA_3850)	-	357772..357978 (+)	207	WP_003714007.1	hypothetical protein	-
NLA_RS02035 (NLA_3860)	pilF	358226..359902 (+)	1677	WP_003710978.1	type IV-A pilus assembly ATPase PilB	Machinery gene
NLA_RS02040 (NLA_3870)	-	359987..360196 (-)	210	WP_013448430.1	DNA gyrase inhibitor YacG	-
NLA_RS02045 (NLA_3880)	coaE	360189..360821 (-)	633	WP_013448431.1	dephospho-CoA kinase	-
NLA_RS02050 (NLA_3890)	pilD	360823..361683 (-)	861	WP_013448432.1	prepilin peptidase	Machinery gene
NLA_RS02055 (NLA_3900)	pilG	361761..362993 (-)	1233	WP_003710974.1	type 4 pilus assembly protein PilG	Machinery gene
NLA_RS02060 (NLA_3910)	pgi	363347..364990 (+)	1644	WP_042508132.1	glucose-6-phosphate isomerase	-
NLA_RS02065	-	364960..365190 (+)	231	WP_036475185.1	hypothetical protein	-
NLA_RS02070 (NLA_3920)	dapD	365200..366021 (+)	822	WP_013448434.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31261.34 Da
• Isoelectric Point: 8.7141

>NTDB_id=39053 NLA_RS02050 WP_013448432.1 360823..361683(-) (pilD) [Neisseria lactamica 020-06]
MFALSVLSPFAVPLAAVLGLLVGSFLNVVIYRVPVMMERGWTVFAKEHLNLPLTDEESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLIAWQYGWSWITLGGLVLTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSLWLLCAVYKLLTGKTGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL

Nucleotide

• Length: 861 bp

>NTDB_id=39053 NLA_RS02050 WP_013448432.1 360823..361683(-) (pilD) [Neisseria lactamica 020-06]
ATGTTTGCTTTGTCTGTACTGTCTCCGTTTGCCGTACCTTTGGCAGCGGTGTTGGGACTGCTGGTCGGCAGTTTCCTGAA
TGTCGTCATTTACCGCGTACCCGTGATGATGGAGCGCGGTTGGACGGTATTTGCCAAAGAACATTTAAACCTGCCTCTGA
CCGATGAGGAAAGCCGTACCTTCAACCTGATGAAGCCGGATTCCTGCTGCCCCAAATGCCGCGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGCTACCTGCTCCTGCGCGGCAAATGCGCCTCCTGCCAAACCAAAATCAGCATACGTTATCC
CTTAATCGAGCTGCTGACCGGCGTATTGTTCGGGCTGATCGCCTGGCAATACGGCTGGTCTTGGATTACGCTGGGCGGAT
TGGTACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACACTGCCC
TTGATTTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATGGTTCATTATGGCTCTTATGTGCAGTGTATAAACTGCTCACAGGAAAAACCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCGCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCAGCATTTTGCCTTCGGTCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACGGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGCTGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (134-242)

3D structure

• AlphaFold DB: E4ZBA2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	97.544	99.65	0.972
pilD	Vibrio cholerae strain A1552	46.739	96.503	0.451
pilD	Acinetobacter baumannii D1279779	48.106	92.308	0.444
pilD	Acinetobacter nosocomialis M2	48.106	92.308	0.444
pilD	Vibrio campbellii strain DS40M4	46.014	96.503	0.444