Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   NLA_RS02035 Genome accession   NC_014752
Coordinates   358226..359902 (+) Length   558 a.a.
NCBI ID   WP_003710978.1    Uniprot ID   D0WCG9
Organism   Neisseria lactamica 020-06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 353226..364902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLA_RS01995 (NLA_3790) rpmG 353424..353579 (+) 156 WP_003689824.1 50S ribosomal protein L33 -
  NLA_RS02000 (NLA_3800) ubiM 353768..354952 (-) 1185 WP_042508131.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  NLA_RS02005 (NLA_3810) rpmA 355337..355609 (-) 273 WP_002212328.1 50S ribosomal protein L27 -
  NLA_RS02010 (NLA_3820) rplU 355634..355942 (-) 309 WP_002216394.1 50S ribosomal protein L21 -
  NLA_RS02015 (NLA_3830) - 356166..357140 (+) 975 WP_003710984.1 polyprenyl synthetase family protein -
  NLA_RS02020 (NLA_3840) - 357145..357591 (+) 447 WP_013448429.1 DUF441 domain-containing protein -
  NLA_RS02030 (NLA_3850) - 357772..357978 (+) 207 WP_003714007.1 hypothetical protein -
  NLA_RS02035 (NLA_3860) pilF 358226..359902 (+) 1677 WP_003710978.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NLA_RS02040 (NLA_3870) - 359987..360196 (-) 210 WP_013448430.1 DNA gyrase inhibitor YacG -
  NLA_RS02045 (NLA_3880) coaE 360189..360821 (-) 633 WP_013448431.1 dephospho-CoA kinase -
  NLA_RS02050 (NLA_3890) pilD 360823..361683 (-) 861 WP_013448432.1 prepilin peptidase Machinery gene
  NLA_RS02055 (NLA_3900) pilG 361761..362993 (-) 1233 WP_003710974.1 type 4 pilus assembly protein PilG Machinery gene

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62047.15 Da        Isoelectric Point: 5.4107

>NTDB_id=39052 NLA_RS02035 WP_003710978.1 358226..359902(+) (pilF) [Neisseria lactamica 020-06]
MSVGLLRTLVQNQVVTVEQAEHYYKESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSVLDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRDNKVFFAVSDPTQMPQIQKTVSAAGIAVELVIVEDDQLAGLLDWVGSRSTSLLQELGEEQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPIDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNEKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEIGFTDEDLAKDWKLY
HAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=39052 NLA_RS02035 WP_003710978.1 358226..359902(+) (pilF) [Neisseria lactamica 020-06]
ATGAGCGTAGGTTTGCTGAGAACTTTGGTTCAAAACCAAGTGGTTACTGTCGAACAGGCCGAACACTATTATAAGGAGTC
GCAGGCGGGTAAAGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCTAAATCCCTTGCGGCACTGATTGCCA
GGGTGTTCAGTTATTCGGTTCTTGATTTGCGCCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAAGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGACAATAAAGTATTTTTTGCGGTTTCCGATCCGACACAGATGCC
GCAAATTCAGAAAACCGTGTCTGCCGCAGGGATTGCGGTCGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGGTCGAGATCGACATCGCTGCTTCAGGAGCTTGGGGAGGAGCAGGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAATGAGGAGGCGGAGGACGGCCCTGTTCCGAGGTTTATCCATAAAACTTTGTCGGATGCCTTGCGCAGCGG
GGCATCGGACATTCATTTTGAGTTTTACGAACACAATGCCCGTATCCGTTTCCGCGTGGACGGGCAGCTTCGCGAGGTGG
TTCAGCCGCCTATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTGATGTCGCGTTTGGATATTTCCGAAAAACGC
ATACCGCAGGACGGCAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTATCGATTTCCGCGTCAGCACTTTGCCGAC
GCTGTTTGGCGAAAAGGTTGTGATGCGGATTTTGAACTCCGATGCCGCGTCTTTGAATATCGACCAGCTCGGTTTTGAGC
CGTTTCAGAAAAAATTGCTGTTGGAAGCGATTCATCGTCCTTACGGTATGGTGCTGGTAACCGGCCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTCAACGTCAATGAGAAGCAGGGCCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGATCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATCAAGGCGGCACAG
ACGGGGCATATGGTGTTTTCCACCCTGCACACCAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGTTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCTTCTGCTCTGAAGGAAATCGGCTTCACCGATGAGGATCTTGCAAAAGATTGGAAACTTTAC
CATGCCGTCGGTTGCGACCGTTGCCGGGGTCAGGGTTACAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAGTTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(178-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D0WCG9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

97.849

100

0.978
  pilB Acinetobacter baumannii D1279779

50.355

100

0.509
  pilB Acinetobacter baylyi ADP1

50.356

100

0.507
  pilB Legionella pneumophila strain ERS1305867

47.703

100

0.484
  pilB Vibrio cholerae strain A1552

45.552

100

0.459
  pilB Vibrio parahaemolyticus RIMD 2210633

45.39

100

0.459
  pilB Vibrio campbellii strain DS40M4

44.149

100

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.179

96.057

0.376

Multiple sequence alignment