Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F8B64_RS04035 Genome accession   NZ_CP044497
Coordinates   773480..774874 (+) Length   464 a.a.
NCBI ID   WP_012054947.1    Uniprot ID   -
Organism   Haemophilus influenzae strain PittGG     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 768480..779874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F8B64_RS04010 (F8B64_04010) rsmE 768495..769232 (-) 738 WP_012054951.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  F8B64_RS04015 (F8B64_04015) lnt 769282..770811 (-) 1530 WP_074039664.1 apolipoprotein N-acyltransferase -
  F8B64_RS04020 (F8B64_04020) corC 770834..771733 (-) 900 WP_005648977.1 CNNM family magnesium/cobalt transport protein CorC -
  F8B64_RS04025 (F8B64_04025) ampD 772356..772919 (-) 564 WP_012054949.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  F8B64_RS04030 (F8B64_04030) pilA 773034..773483 (+) 450 WP_012054948.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  F8B64_RS04035 (F8B64_04035) pilB 773480..774874 (+) 1395 WP_012054947.1 GspE/PulE family protein Machinery gene
  F8B64_RS04040 (F8B64_04040) pilC 774871..776091 (+) 1221 WP_012054946.1 type II secretion system F family protein Machinery gene
  F8B64_RS04045 (F8B64_04045) pilD 776088..776780 (+) 693 WP_005666688.1 A24 family peptidase Machinery gene
  F8B64_RS04050 (F8B64_04050) rho 776835..778097 (-) 1263 WP_005666690.1 transcription termination factor Rho -
  F8B64_RS04055 (F8B64_04055) metJ 778345..778662 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  F8B64_RS04060 (F8B64_04060) cueR 778676..779062 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  F8B64_RS04065 (F8B64_04065) - 779139..779345 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  F8B64_RS04070 (F8B64_04070) - 779419..779625 (+) 207 WP_012054944.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52926.39 Da        Isoelectric Point: 5.8398

>NTDB_id=390513 F8B64_RS04035 WP_012054947.1 773480..774874(+) (pilB) [Haemophilus influenzae strain PittGG]
MTSYALLHTQRVTAKNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFNRLLR
TFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCNQGYQGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVNQGMTDNKELERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=390513 F8B64_RS04035 WP_012054947.1 773480..774874(+) (pilB) [Haemophilus influenzae strain PittGG]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTAAAAATGGCGAAGTATTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTACGTTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCCTGTGAAACCATTGCGTTTATAACGGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAATTGTTACAACAACTTACTCCGCGTCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACTTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAGAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACAGAAAATCAGCAACAAGCATTTCAACGTGCGCTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACTGCTGAAGATC
CCATTGAAATTGAGCTGGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAACCGCCTATTGCGT
ACTTTTTTACGCCAAGATCCCGATATCATTATGTTAGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAAACCGGGCATTTAGTACTCTCAACTTTACATACCAATGATGCGATATCTGCCATTTCTCGATTACAACAACTTG
GTATTCAACAGTATGAAATTGAAAACAGCTTACTGCTCGTTATTGCGCAACGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCAATCAAGGTTATCAAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACGGATTTTAAAAATTTACATGCGAGTGGTTTAGAAAAAGTTAATCAAGGAATGACGGATAATA
AAGAACTTGAACGTGTGCTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-97)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

96.976

99.784

0.968
  pilB Haemophilus influenzae 86-028NP

96.121

100

0.961
  pilB Glaesserella parasuis strain SC1401

57.08

97.414

0.556
  pilB Vibrio cholerae strain A1552

39.469

100

0.448
  pilB Vibrio campbellii strain DS40M4

40.491

100

0.427
  pilB Vibrio parahaemolyticus RIMD 2210633

40.373

100

0.42
  pilB Legionella pneumophila strain ERS1305867

38.945

100

0.414
  pilB Acinetobacter baylyi ADP1

37.903

100

0.405
  pilB Acinetobacter baumannii D1279779

38.669

100

0.401
  pilF Neisseria gonorrhoeae MS11

36.345

100

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.966

100

0.373
  pilF Thermus thermophilus HB27

35.611

100

0.371

Multiple sequence alignment