Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   F8B64_RS04030 Genome accession   NZ_CP044497
Coordinates   773034..773483 (+) Length   149 a.a.
NCBI ID   WP_012054948.1    Uniprot ID   A0AAX3ITG0
Organism   Haemophilus influenzae strain PittGG     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 768034..778483
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F8B64_RS04010 (F8B64_04010) rsmE 768495..769232 (-) 738 WP_012054951.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  F8B64_RS04015 (F8B64_04015) lnt 769282..770811 (-) 1530 WP_074039664.1 apolipoprotein N-acyltransferase -
  F8B64_RS04020 (F8B64_04020) corC 770834..771733 (-) 900 WP_005648977.1 CNNM family magnesium/cobalt transport protein CorC -
  F8B64_RS04025 (F8B64_04025) ampD 772356..772919 (-) 564 WP_012054949.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  F8B64_RS04030 (F8B64_04030) pilA 773034..773483 (+) 450 WP_012054948.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  F8B64_RS04035 (F8B64_04035) pilB 773480..774874 (+) 1395 WP_012054947.1 GspE/PulE family protein Machinery gene
  F8B64_RS04040 (F8B64_04040) pilC 774871..776091 (+) 1221 WP_012054946.1 type II secretion system F family protein Machinery gene
  F8B64_RS04045 (F8B64_04045) pilD 776088..776780 (+) 693 WP_005666688.1 A24 family peptidase Machinery gene
  F8B64_RS04050 (F8B64_04050) rho 776835..778097 (-) 1263 WP_005666690.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15523.99 Da        Isoelectric Point: 9.6179

>NTDB_id=390512 F8B64_RS04030 WP_012054948.1 773034..773483(+) (pilA) [Haemophilus influenzae strain PittGG]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTGKPSTCSGGSNGIA
ADITTAKGYVASVKTQSGGITVKGNGTLANMEYILQAKGNATAGVTWTTTCKGTDASLFPANFCRSVTK

Nucleotide


Download         Length: 450 bp        

>NTDB_id=390512 F8B64_RS04030 WP_012054948.1 773034..773483(+) (pilA) [Haemophilus influenzae strain PittGG]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCCTCTTATCAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCTTCTGCGC
CTTATAAGGCTGATGTGGAATTATGCGTTTATAGCACAGGCAAACCTTCTACTTGCTCAGGAGGAAGCAATGGAATTGCA
GCTGATATTACAACAGCAAAAGGCTATGTAGCCTCAGTGAAAACTCAATCAGGTGGTATTACAGTAAAAGGGAATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTAAAGGTAATGCTACAGCAGGTGTAACTTGGACAACAACCTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCAGAAGTGTCACAAAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

91.946

100

0.919
  pilA Haemophilus influenzae 86-028NP

87.919

100

0.879
  pilA Glaesserella parasuis strain SC1401

56.757

99.329

0.564
  pilA2 Legionella pneumophila strain ERS1305867

39.735

100

0.403
  pilA2 Legionella pneumophila str. Paris

40.559

95.973

0.389
  comP Acinetobacter baylyi ADP1

38.621

97.315

0.376
  pilA Vibrio campbellii strain DS40M4

39.007

94.631

0.369

Multiple sequence alignment