Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FOB71_RS20880 Genome accession   NZ_CP044069
Coordinates   2796705..2798390 (+) Length   561 a.a.
NCBI ID   WP_017421193.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FDAARGOS_663     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2791705..2803390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB71_RS20860 (FOB71_20855) pdhR 2793320..2794087 (-) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  FOB71_RS20865 (FOB71_20860) ampD 2794476..2795048 (-) 573 WP_017421196.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FOB71_RS20870 (FOB71_20865) nadC 2795141..2796028 (+) 888 WP_017421195.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FOB71_RS20875 (FOB71_20870) - 2796251..2796700 (+) 450 WP_176309687.1 pilin -
  FOB71_RS20880 (FOB71_20875) pilB 2796705..2798390 (+) 1686 WP_017421193.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FOB71_RS20885 (FOB71_20880) pilC 2798438..2799664 (+) 1227 WP_017421192.1 type II secretion system F family protein Machinery gene
  FOB71_RS20890 (FOB71_20885) pilD 2799758..2800627 (+) 870 WP_026060802.1 A24 family peptidase Machinery gene
  FOB71_RS20895 (FOB71_20890) coaE 2800629..2801237 (+) 609 WP_017421190.1 dephospho-CoA kinase -
  FOB71_RS20900 (FOB71_20895) zapD 2801270..2802010 (+) 741 WP_017421189.1 cell division protein ZapD -
  FOB71_RS20905 (FOB71_20900) yacG 2802073..2802267 (+) 195 WP_026060801.1 DNA gyrase inhibitor YacG -
  FOB71_RS20910 (FOB71_20905) rplS 2802579..2802932 (-) 354 WP_011079544.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62342.33 Da        Isoelectric Point: 5.6848

>NTDB_id=387386 FOB71_RS20880 WP_017421193.1 2796705..2798390(+) (pilB) [Vibrio vulnificus strain FDAARGOS_663]
MTNLATILRQANQLSLTQEQACRENIQASGVTAPEALLQLGFFQPDELTEKLSAIFGLPRTDLNQYDYQELCNQLGLREL
ITRHQALPLQRSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQDLQAAIRRLYGKSIKQSQGSGLKEIHQDELAN
LVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGMI
LMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADDL
IYQADRQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=387386 FOB71_RS20880 WP_017421193.1 2796705..2798390(+) (pilB) [Vibrio vulnificus strain FDAARGOS_663]
ATGACTAACCTTGCCACAATTCTTCGTCAGGCCAATCAACTCAGCCTAACGCAAGAACAAGCGTGTCGAGAAAACATCCA
AGCTTCTGGTGTGACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCTGATGAACTAACCGAAAAGCTAAGTG
CGATTTTTGGCTTACCACGCACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGGTTGCGTGAGCTG
ATCACTCGTCACCAAGCACTGCCACTGCAAAGAAGCAACGCTGGTTTAATACTCGCGGTTGCAGACCCGACCAATATTCA
AGCTGAAGATGATTTCCGTTTTGCCACAGGCGTGCAGGTGGAGATTGTTTTAGCCGATTGTCAAGATCTGCAAGCGGCGA
TTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAGGCTCTGGTTTAAAAGAGATTCACCAAGATGAACTGGCGAAT
TTGGTCGAGGTTTCAGATGACGAAGTCGAGTCCATTGAAGACTTAAGCCAAGATGAATCCCCCGTTAGCCGCTTTATCAA
TCAAATTCTGCTTGATGCGGTGCGCAAAGGCGCGTCCGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTACGAT
TGCGTTGCGATGGTATTTTGGTGGAAGTGCAACAGCCTCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAAATC
CTCTCCAAGCTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGCATTAAGCTACGCCTTAACCAAGAAACCGCGAT
TGATATGCGCGTTTCCACTTTACCTACGCTGTTTGGTGAAAAAATTGTGCTGCGACTTCTCGACAGCTCGAGTAGCAGCT
TGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGAATGATT
TTGATGACAGGTCCAACGGGCAGCGGCAAAACCGTTTCGCTGTACACAGGCTTGAGTTTGCTTAACCGACCTGAAGTGAA
TATCTCCACCGCGGAAGACCCGGTCGAAATTAACCTCCCGGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTTTTG
GTTTTGCCGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAAACC
GCCGAGATCGCCGTCAAAGCAGCCCAAACCGGCCACCTTGTGCTATCCACCTTACATACCAACTCGGCTGCTGAAACCAT
TGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTGGCTTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGGCTC
GTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCAACATTTGAGTTACTCGGTCTAAAAGCTGACGACTTG
ATTTATCAAGCTGATAGACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCATGCC
TTTTGATGAACAGCTTGCAGAGGCGATAGTGAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTCGCTCGGCAAAAAGGCA
TGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGTTTAGAAGAGCTGCAACGCGTCCTCTAT
TTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.649

100

0.766
  pilB Vibrio parahaemolyticus RIMD 2210633

75.893

99.822

0.758
  pilB Vibrio cholerae strain A1552

74.199

100

0.743
  pilB Legionella pneumophila strain ERS1305867

53.216

91.444

0.487
  pilB Acinetobacter baumannii D1279779

51.538

92.692

0.478
  pilB Acinetobacter baylyi ADP1

50.478

93.226

0.471
  pilF Neisseria gonorrhoeae MS11

45.633

100

0.456
  pilF Thermus thermophilus HB27

40.215

99.287

0.399
  pilB/pilB1 Synechocystis sp. PCC 6803

37.063

100

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.679

94.474

0.365
  pilB Haemophilus influenzae Rd KW20

41.736

86.275

0.36

Multiple sequence alignment