Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F1539_RS05375 Genome accession   NZ_CP043772
Coordinates   1023945..1025339 (+) Length   464 a.a.
NCBI ID   WP_013525533.1    Uniprot ID   A0AAV2U5G9
Organism   Haemophilus influenzae biotype aegyptius strain HE24/F3037     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1018945..1030339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F1539_RS05350 (F1539_05355) rsmE 1018963..1019700 (-) 738 WP_006996180.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  F1539_RS05355 (F1539_05360) lnt 1019750..1021279 (-) 1530 WP_013525535.1 apolipoprotein N-acyltransferase -
  F1539_RS05360 (F1539_05365) corC 1021302..1022201 (-) 900 WP_013525534.1 CNNM family magnesium/cobalt transport protein CorC -
  F1539_RS05365 (F1539_05370) ampD 1022824..1023384 (-) 561 WP_006996183.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  F1539_RS05370 (F1539_05375) pilA 1023499..1023948 (+) 450 WP_006996184.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  F1539_RS05375 (F1539_05380) pilB 1023945..1025339 (+) 1395 WP_013525533.1 GspE/PulE family protein Machinery gene
  F1539_RS05380 (F1539_05385) - 1025336..1026553 (+) 1218 Protein_1052 type II secretion system F family protein -
  F1539_RS05385 (F1539_05390) pilD 1026550..1027242 (+) 693 WP_013525531.1 A24 family peptidase Machinery gene
  F1539_RS05390 (F1539_05395) rho 1027297..1028559 (-) 1263 WP_005666690.1 transcription termination factor Rho -
  F1539_RS05395 (F1539_05400) metJ 1028807..1029124 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  F1539_RS05400 (F1539_05405) cueR 1029138..1029524 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  F1539_RS05405 (F1539_05410) - 1029601..1029807 (+) 207 WP_013525530.1 heavy-metal-associated domain-containing protein -
  F1539_RS05410 (F1539_05415) - 1029881..1030087 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52905.44 Da        Isoelectric Point: 5.8398

>NTDB_id=385838 F1539_RS05375 WP_013525533.1 1023945..1025339(+) (pilB) [Haemophilus influenzae biotype aegyptius strain HE24/F3037]
MTSYALLHTQRVTAQNGEIFTISPDLWVRNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFQNLRVSGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=385838 F1539_RS05375 WP_013525533.1 1023945..1025339(+) (pilB) [Haemophilus influenzae biotype aegyptius strain HE24/F3037]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACGATCTCGCCTGATTTATG
GGTGCGTAATCAACAACAGCAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCGCCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATAGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTAACTGGCCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACCGCTGAGGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAACCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGC
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGTGATGAAGAAAGTGCGATGATTGCACTACGTGC
CGCTCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTTGTCATTGCACAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACAGATTTTCAAAATTTACGTGTAAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-395)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

98.491

100

0.985
  pilB Haemophilus influenzae Rd KW20

96.76

99.784

0.966
  pilB Glaesserella parasuis strain SC1401

57.675

98.276

0.567
  pilB Vibrio cholerae strain A1552

39.848

100

0.453
  pilB Legionella pneumophila strain ERS1305867

39.757

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

40.373

100

0.42
  pilB Vibrio campbellii strain DS40M4

40.206

100

0.42
  pilB Acinetobacter baylyi ADP1

38.306

100

0.409
  pilB Acinetobacter baumannii D1279779

38.877

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.853

100

0.384
  pilF Neisseria gonorrhoeae MS11

36.975

100

0.379
  pilF Thermus thermophilus HB27

35.924

100

0.369

Multiple sequence alignment