Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   F1539_RS05370 Genome accession   NZ_CP043772
Coordinates   1023499..1023948 (+) Length   149 a.a.
NCBI ID   WP_006996184.1    Uniprot ID   A0AAV2U2D0
Organism   Haemophilus influenzae biotype aegyptius strain HE24/F3037     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1018499..1028948
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F1539_RS05350 (F1539_05355) rsmE 1018963..1019700 (-) 738 WP_006996180.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  F1539_RS05355 (F1539_05360) lnt 1019750..1021279 (-) 1530 WP_013525535.1 apolipoprotein N-acyltransferase -
  F1539_RS05360 (F1539_05365) corC 1021302..1022201 (-) 900 WP_013525534.1 CNNM family magnesium/cobalt transport protein CorC -
  F1539_RS05365 (F1539_05370) ampD 1022824..1023384 (-) 561 WP_006996183.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  F1539_RS05370 (F1539_05375) pilA 1023499..1023948 (+) 450 WP_006996184.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  F1539_RS05375 (F1539_05380) pilB 1023945..1025339 (+) 1395 WP_013525533.1 GspE/PulE family protein Machinery gene
  F1539_RS05380 (F1539_05385) - 1025336..1026553 (+) 1218 Protein_1052 type II secretion system F family protein -
  F1539_RS05385 (F1539_05390) pilD 1026550..1027242 (+) 693 WP_013525531.1 A24 family peptidase Machinery gene
  F1539_RS05390 (F1539_05395) rho 1027297..1028559 (-) 1263 WP_005666690.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15542.86 Da        Isoelectric Point: 8.0922

>NTDB_id=385837 F1539_RS05370 WP_006996184.1 1023499..1023948(+) (pilA) [Haemophilus influenzae biotype aegyptius strain HE24/F3037]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTNETTNCTGGKNGIA
ADITAAKGYVKSVTTSNGAITVKGDGTLANMEYILQATGNAATGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=385837 F1539_RS05370 WP_006996184.1 1023499..1023948(+) (pilA) [Haemophilus influenzae biotype aegyptius strain HE24/F3037]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCCTCTTATCAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGCACAAATGAAACAACAAACTGTACGGGTGGAAAAAATGGTATTGCA
GCAGATATAACCGCAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAACAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTTACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

99.329

100

0.993
  pilA Haemophilus influenzae Rd KW20

91.275

100

0.913
  pilA Glaesserella parasuis strain SC1401

54.362

100

0.544
  pilA2 Legionella pneumophila str. Paris

42.177

98.658

0.416
  pilA2 Legionella pneumophila strain ERS1305867

42.177

98.658

0.416
  pilA Vibrio campbellii strain DS40M4

39.716

94.631

0.376

Multiple sequence alignment