Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   F0316_RS02175 Genome accession   NZ_CP043554
Coordinates   435646..436872 (-) Length   408 a.a.
NCBI ID   WP_119779472.1    Uniprot ID   -
Organism   Vibrio cholerae strain RFB16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 430646..441872
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0316_RS02150 (F0316_02150) parC 430647..432932 (+) 2286 WP_149560166.1 DNA topoisomerase IV subunit A -
  F0316_RS02155 (F0316_02155) yacG 432981..433178 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  F0316_RS02160 (F0316_02160) zapD 433336..434076 (-) 741 WP_000207194.1 cell division protein ZapD -
  F0316_RS02165 (F0316_02165) coaE 434108..434716 (-) 609 WP_000011557.1 dephospho-CoA kinase -
  F0316_RS02170 (F0316_02170) pilD 434713..435588 (-) 876 WP_000418747.1 A24 family peptidase Machinery gene
  F0316_RS02175 (F0316_02175) pilC 435646..436872 (-) 1227 WP_119779472.1 type II secretion system F family protein Machinery gene
  F0316_RS02180 (F0316_02180) pilB 436915..438603 (-) 1689 WP_149560167.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F0316_RS02185 (F0316_02185) pilA 438610..439032 (-) 423 WP_000649339.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  F0316_RS02190 (F0316_02190) nadC 439277..440167 (-) 891 WP_000665267.1 carboxylating nicotinate-nucleotide diphosphorylase -
  F0316_RS02195 (F0316_02195) ampD 440465..441010 (+) 546 WP_000567318.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  F0316_RS02200 (F0316_02200) fldB 441305..441823 (-) 519 WP_000690112.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45164.46 Da        Isoelectric Point: 10.3424

>NTDB_id=385045 F0316_RS02175 WP_119779472.1 435646..436872(-) (pilC) [Vibrio cholerae strain RFB16]
MKATQTLPLKNYRWKGINSNGKKVSGQMLAISEIEVRDKLKDQHIQIKKLKKGSVSLLARLTHRVKSKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKSVEAGTPLSKAMRTASAHFDTLYVDLVETGEMSGNLPEVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQVLKLSHWVQAYSLWAFIAIAATIFG
LKALRKNSFQIRLKTSRLGLKFPIIGNVLAKASIAKFSRTLATSFAAGIPILASLKTTAKTSGNVHFETAINEVYRDTAA
GMPMYIAMRNTEAFPEMVLQMVMIGEESGQLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=385045 F0316_RS02175 WP_119779472.1 435646..436872(-) (pilC) [Vibrio cholerae strain RFB16]
ATGAAAGCGACCCAAACCTTACCTCTGAAAAATTATCGCTGGAAAGGCATCAACAGCAACGGCAAAAAAGTTTCCGGCCA
GATGCTCGCCATCTCCGAAATCGAGGTGCGCGATAAGCTCAAAGATCAGCATATTCAGATCAAAAAACTGAAAAAAGGCA
GTGTCTCTTTATTGGCGCGCCTAACCCATCGCGTGAAAAGCAAAGACATCACGATTTTGACTCGGCAGTTGGCGACCATG
CTCACCACGGGCGTACCGATTGTGCAAGCCCTCAAGTTGGTGGGCGATAATCACCGTAAAGCTGAGATGAAATCGATTCT
GGCGCAAATCACCAAAAGCGTGGAAGCGGGCACGCCGCTTTCCAAGGCGATGCGCACCGCCAGCGCCCATTTTGATACCT
TGTATGTCGACTTAGTCGAAACCGGAGAGATGTCCGGTAACTTACCTGAGGTGTTTGAGCGTTTGGCCACCTACCGCGAG
AAAAGCGAGCAGCTACGCGCCAAGGTGATCAAAGCGCTCATCTACCCGAGTATGGTAGTGTTGGTCGCGCTCGGAGTTTC
CTACTTAATGCTCACCATGGTCATCCCAGAATTTGAAAGCATGTTTAAAGGCTTTGGTGCTGAACTGCCTTGGTTTACGC
AGCAAGTGCTGAAACTCTCACACTGGGTGCAGGCTTACAGTTTATGGGCATTTATCGCCATCGCAGCAACCATTTTTGGC
TTGAAAGCGCTGCGTAAAAACTCCTTCCAGATCCGTTTGAAAACCAGTCGCTTGGGGCTGAAATTTCCGATTATTGGCAA
TGTACTCGCCAAAGCGTCCATCGCCAAATTCAGCCGCACCCTCGCCACCAGCTTTGCTGCGGGGATCCCGATCCTCGCCA
GTTTAAAAACCACGGCCAAAACCTCCGGCAATGTGCACTTTGAAACCGCGATTAATGAGGTGTACCGCGATACCGCTGCG
GGTATGCCGATGTACATTGCCATGCGCAATACCGAAGCTTTTCCCGAAATGGTGCTGCAAATGGTGATGATCGGTGAAGA
GTCTGGTCAATTAGATGACATGCTCAACAAGGTCGCGACCATCTATGAATTTGAAGTCGATAACACGGTCGATAACTTGG
GCAAGATTCTTGAACCACTGATCATCGTCTTTCTTGGGACAGTCGTGGGCGGCTTAGTGGTGGCGATGTACTTACCTATC
TTTAACTTAATGAGTGTATTGGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

99.755

100

0.998
  pilC Vibrio campbellii strain DS40M4

73.75

98.039

0.723
  pilC Acinetobacter baumannii D1279779

42.647

100

0.426
  pilC Acinetobacter baylyi ADP1

41.769

99.755

0.417
  pilC Legionella pneumophila strain ERS1305867

42.211

97.549

0.412
  pilC Pseudomonas stutzeri DSM 10701

42.105

97.794

0.412
  pilG Neisseria gonorrhoeae MS11

41.045

98.529

0.404
  pilG Neisseria meningitidis 44/76-A

40.547

98.529

0.4

Multiple sequence alignment