Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F0316_RS02180 Genome accession   NZ_CP043554
Coordinates   436915..438603 (-) Length   562 a.a.
NCBI ID   WP_149560167.1    Uniprot ID   -
Organism   Vibrio cholerae strain RFB16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 431915..443603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0316_RS02155 (F0316_02155) yacG 432981..433178 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  F0316_RS02160 (F0316_02160) zapD 433336..434076 (-) 741 WP_000207194.1 cell division protein ZapD -
  F0316_RS02165 (F0316_02165) coaE 434108..434716 (-) 609 WP_000011557.1 dephospho-CoA kinase -
  F0316_RS02170 (F0316_02170) pilD 434713..435588 (-) 876 WP_000418747.1 A24 family peptidase Machinery gene
  F0316_RS02175 (F0316_02175) pilC 435646..436872 (-) 1227 WP_119779472.1 type II secretion system F family protein Machinery gene
  F0316_RS02180 (F0316_02180) pilB 436915..438603 (-) 1689 WP_149560167.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F0316_RS02185 (F0316_02185) pilA 438610..439032 (-) 423 WP_000649339.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  F0316_RS02190 (F0316_02190) nadC 439277..440167 (-) 891 WP_000665267.1 carboxylating nicotinate-nucleotide diphosphorylase -
  F0316_RS02195 (F0316_02195) ampD 440465..441010 (+) 546 WP_000567318.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  F0316_RS02200 (F0316_02200) fldB 441305..441823 (-) 519 WP_000690112.1 flavodoxin FldB -
  F0316_RS02205 (F0316_02205) xerD 441961..442869 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62021.12 Da        Isoelectric Point: 5.9438

>NTDB_id=385046 F0316_RS02180 WP_149560167.1 436915..438603(-) (pilB) [Vibrio cholerae strain RFB16]
MLTNLVAILRQAELISATQEQAVVTQVSASGTSVPEALLGLGIFSPQELTEQLSHIFGLPETDLSRYDYAPLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVSDDELQSIEDLSQDESPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=385046 F0316_RS02180 WP_149560167.1 436915..438603(-) (pilB) [Vibrio cholerae strain RFB16]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGCAACGCAAGAACAAGCGGTGGTTACGCAGGT
TAGCGCCTCGGGGACGTCGGTGCCCGAGGCGCTGCTTGGTTTAGGGATTTTCTCCCCCCAAGAACTCACCGAACAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCCCCCTGTGCCAACAGCTTGGGCTGCGTGAA
CTGATTACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTATTGCTGCTCGCCGTCTCTGACCCGACCTTACT
GCAAGCCGAAGAAGAGTTTCGTTTTGCCACAGGATTACAAGTTGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCGG
CGATTCGCCGTTTGTATGGCCGCTCGATTCAAGGCGCTGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCAAT
CTGGTTAAAGTCAGTGATGACGAGCTGCAATCTATTGAAGATCTCAGCCAAGATGAATCCCCCGTCAGTCGCTTTATCAA
CCAAGTGCTGCTCGATGCGGTACGTAAAGGTGCCTCGGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGGATCTTGGTCGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCCGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGCTTGCCACAAGATGGCCGGATTAAACTGCGCCTAAGCCGCGATACCGCCAT
TGATATGCGTGTATCCACGCTTCCCACTTTATGGGGAGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCTAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTATCTCAACGCCCTCAAAAGACCGCAAGGGATGATT
TTGATGACCGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTTAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTGGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCTGATGTAGTGATGGTCGGCGAAATACGCGATCTGGAAACT
GCAGAAATCGCGGTAAAAGCCGCACAAACCGGTCACTTGGTGCTTTCCACCCTGCACACCAACTCAGCCGCTGAAACCGT
AATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAACCTTGCGTCATCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCCTATGTAAACATTGCAAAATCGCGGTGCGCCCTTCTGCCCTATTGCAAAGCCAATTTGCGTTTCAGCCCAATGAA
ATCTTGTATGAAGCCAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAACGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

98.932

100

0.989
  pilB Vibrio parahaemolyticus RIMD 2210633

74.021

100

0.74
  pilB Vibrio campbellii strain DS40M4

73.843

100

0.738
  pilB Legionella pneumophila strain ERS1305867

51.619

93.416

0.482
  pilB Acinetobacter baylyi ADP1

50.672

92.705

0.47
  pilB Acinetobacter baumannii D1279779

50.383

92.883

0.468
  pilF Neisseria gonorrhoeae MS11

45.73

100

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

37.252

100

0.4
  pilF Thermus thermophilus HB27

39.556

96.263

0.381

Multiple sequence alignment