Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   F0316_RS01145 Genome accession   NZ_CP043554
Coordinates   233302..235017 (+) Length   571 a.a.
NCBI ID   WP_000788423.1    Uniprot ID   A0A6B3LC57
Organism   Vibrio cholerae strain RFB16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 228302..240017
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0316_RS01125 (F0316_01125) pilM 230595..231611 (+) 1017 WP_000520443.1 type IV pilus assembly protein PilM Machinery gene
  F0316_RS01130 (F0316_01130) pilN 231601..232185 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  F0316_RS01135 (F0316_01135) pilO 232178..232768 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  F0316_RS01140 (F0316_01140) pilP 232758..233276 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  F0316_RS01145 (F0316_01145) pilQ 233302..235017 (+) 1716 WP_000788423.1 type IV pilus secretin PilQ Machinery gene
  F0316_RS01150 (F0316_01150) aroK 235204..235728 (+) 525 WP_000818616.1 shikimate kinase AroK -
  F0316_RS01155 (F0316_01155) aroB 235756..236841 (+) 1086 WP_053055863.1 3-dehydroquinate synthase -
  F0316_RS01160 (F0316_01160) - 236896..238371 (+) 1476 WP_000050190.1 AAA family ATPase -
  F0316_RS01165 (F0316_01165) - 238441..239274 (+) 834 WP_000744680.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62374.50 Da        Isoelectric Point: 4.6119

>NTDB_id=385042 F0316_RS01145 WP_000788423.1 233302..235017(+) (pilQ) [Vibrio cholerae strain RFB16]
MKNGLKTYVAQTWLTLWVGFALCASSMVFSAESATANQLENIDFRVNKEKAAVLIVELASPSAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVESVEVFRKEPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLEELGVRWGVMSTNGSHSVGGSIESNLWQKGLLADDEFPVDEFLNVNLASTSANASSIAFQVAKLG
SGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=385042 F0316_RS01145 WP_000788423.1 233302..235017(+) (pilQ) [Vibrio cholerae strain RFB16]
ATGAAAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTGTGGGTTGGTTTTGCACTTTGTGCTTCTTCCAT
GGTGTTTAGTGCTGAATCCGCTACCGCCAACCAGTTGGAAAACATCGACTTTCGGGTCAACAAAGAGAAGGCGGCGGTCT
TAATTGTCGAATTGGCTTCGCCATCGGCTGTCGTGGATGTGCAAAAAGTCCAAGAAGGGTTGAGCATTGAACTCCTGAAA
ACCGATGTGGCGGATGACAAGCTGTATCTGCTTGATGTGAAAGATTTTTCGACCCCTGTTGAGAGCGTGGAAGTATTCCG
TAAAGAGCCAAGCACACAACTGGTTGTGACTGTCGATGGGGAGTTTCAGCATGATTATACCTTGAAAGGAAAATACCTTG
AGGTCGTGATCAGTAAACTCAAAGCTGATGAAAAGCCTAAGCCCAAGAGTGTCTTGGAAAAAGAAGGCAAGCTGATTTCA
ATTAACTTCCAAGATATTCCGGTGCGTAACGTACTGCAGCTGATTGCCGATTATAACGGTTTCAACTTAGTGGTTTCCGA
TTCGGTGGTGGGTAACCTGACTTTACGCTTGGATGGCGTGCCTTGGCAGCAAGTGTTGGACATTATTCTGCAAGTCAAAG
GGCTCGATAAGCGGGTCGATGGCAATGTGATTTTGATTGCGCCGAAAGAGGAACTCGACTTGCGCGAGAAACAAGCGCTC
GAAAAAGCCCGTTTAGCAGAAGAGTTGGGCGATTTAAAATCAGAAATCATCAAGATTAACTTTGCCAAAGCGTCTGACAT
TGCGGCGATGATCGGCGGTGAAGGCAACGTCAACATGTTGTCTGAACGAGGTTCGATCAGCATCGACGAACGAACTAACT
CGCTATTGATCCGAGAGCTGCCCGATAACATTGCCGTGATCCGCGAGATCATTGAATCGCTGGATATTCCGGTCAAACAA
GTTCAAATCGAAGCGCGGATTGTCACCGTCAAAGAAGGTAACTTGGAGGAGCTGGGGGTACGCTGGGGCGTAATGTCGAC
CAATGGTAGCCACTCGGTCGGAGGAAGCATAGAGAGTAACTTATGGCAAAAAGGGTTATTGGCGGATGATGAATTTCCGG
TGGATGAATTCTTAAATGTGAATTTGGCTTCCACCTCTGCCAATGCGTCTTCGATTGCTTTCCAAGTCGCTAAATTGGGC
TCGGGTACGTTGTTGGATTTGGAGCTTTCAGCATTACAAAATGAATCGAAGGCCGAAATCATCTCTAGCCCTCGCTTGAT
TACCACCAATAAACAGCCTGCCTATATTGAGCAAGGTACTGAGATCCCTTATCTGGAATCGTCTTCCAGTGGTGCCTCTA
CGGTCGCGTTTAAAAAAGCGGTATTGAGCTTGAAGGTGACACCACAAATCACCCCAGATAACCGCTTGGTGCTGGACTTG
AGCGTGACCCAAGACCGACGCGGTGAAACGGTGAAAACCGGCACTGGCGAAGCGGTTTCTATTGATACTCAGCGCATTGG
CACTCAAGTGTTGGTGAATAACGGAGAAACTGTGGTGTTGGGTGGCATTTTCCAACACAGCATCAATAATTCGGTAGATA
AGGTGCCACTGCTGGGGGATTTGCCTGTTTTGGGCGCGTTATTCAGACGAACCTATGAGCAAATGGGCAAAAGTGAACTG
CTGATTTTTGTCACGCCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(411-569)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6B3LC57

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

99.825

100

0.998
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

99.825

100

0.998
  pilQ Vibrio campbellii strain DS40M4

78.545

96.322

0.757
  pilQ Legionella pneumophila strain ERS1305867

42.778

94.571

0.405
  pilQ Legionella pneumophila strain Lp02

42.778

94.571

0.405
  comQ Acinetobacter baylyi ADP1

39.336

100

0.394
  pilQ Acinetobacter baumannii strain A118

37.819

100

0.389
  pilQ Acinetobacter baumannii D1279779

37.819

100

0.389
  pilQ Pseudomonas aeruginosa PAK

39.344

96.147

0.378

Multiple sequence alignment