Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   F0316_RS01125 Genome accession   NZ_CP043554
Coordinates   230595..231611 (+) Length   338 a.a.
NCBI ID   WP_000520443.1    Uniprot ID   -
Organism   Vibrio cholerae strain RFB16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 225595..236611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0316_RS01110 (F0316_01110) - 226136..226867 (-) 732 WP_000495223.1 glutathione peroxidase -
  F0316_RS01115 (F0316_01115) oxyR 227007..227900 (+) 894 WP_149560152.1 DNA-binding transcriptional regulator OxyR -
  F0316_RS01120 (F0316_01120) - 227975..230452 (-) 2478 WP_162808121.1 penicillin-binding protein 1A -
  F0316_RS01125 (F0316_01125) pilM 230595..231611 (+) 1017 WP_000520443.1 type IV pilus assembly protein PilM Machinery gene
  F0316_RS01130 (F0316_01130) pilN 231601..232185 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  F0316_RS01135 (F0316_01135) pilO 232178..232768 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  F0316_RS01140 (F0316_01140) pilP 232758..233276 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  F0316_RS01145 (F0316_01145) pilQ 233302..235017 (+) 1716 WP_000788423.1 type IV pilus secretin PilQ Machinery gene
  F0316_RS01150 (F0316_01150) aroK 235204..235728 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37688.39 Da        Isoelectric Point: 7.1492

>NTDB_id=385038 F0316_RS01125 WP_000520443.1 230595..231611(+) (pilM) [Vibrio cholerae strain RFB16]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKHPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=385038 F0316_RS01125 WP_000520443.1 230595..231611(+) (pilM) [Vibrio cholerae strain RFB16]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCAGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACACCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.704

100

0.997
  pilM Vibrio campbellii strain DS40M4

48.225

100

0.482

Multiple sequence alignment