Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   FXO12_RS13770 Genome accession   NZ_CP043060
Coordinates   3054682..3055890 (+) Length   402 a.a.
NCBI ID   WP_057976748.1    Uniprot ID   A0A4Q0HS76
Organism   Pseudomonas sp. J380     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3049682..3060890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FXO12_RS13735 (FXO12_13735) - 3049833..3050321 (-) 489 WP_160049698.1 hypothetical protein -
  FXO12_RS13740 (FXO12_13740) trfA 3050583..3051515 (-) 933 WP_244251342.1 plasmid replication initiator TrfA -
  FXO12_RS13745 (FXO12_13745) - 3051473..3051682 (-) 210 WP_160049699.1 AlpA family transcriptional regulator -
  FXO12_RS13750 (FXO12_13750) - 3051798..3052547 (-) 750 WP_160049700.1 hypothetical protein -
  FXO12_RS13755 (FXO12_13755) - 3052544..3053761 (-) 1218 WP_160049701.1 tyrosine-type recombinase/integrase -
  FXO12_RS13765 (FXO12_13765) - 3054067..3054480 (-) 414 WP_057976751.1 pilin -
  FXO12_RS13770 (FXO12_13770) pilC 3054682..3055890 (+) 1209 WP_057976748.1 type II secretion system F family protein Machinery gene
  FXO12_RS13775 (FXO12_13775) pilD 3055887..3056753 (+) 867 WP_057976746.1 A24 family peptidase Machinery gene
  FXO12_RS13780 (FXO12_13780) coaE 3056750..3057373 (+) 624 WP_057976744.1 dephospho-CoA kinase -
  FXO12_RS13785 (FXO12_13785) yacG 3057370..3057570 (+) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  FXO12_RS13790 (FXO12_13790) - 3057567..3057782 (-) 216 WP_003171680.1 hypothetical protein -
  FXO12_RS13795 (FXO12_13795) - 3057847..3058536 (-) 690 WP_054919398.1 energy-coupling factor ABC transporter permease -
  FXO12_RS13800 (FXO12_13800) - 3058665..3059294 (+) 630 WP_003188431.1 DUF1780 domain-containing protein -
  FXO12_RS13805 (FXO12_13805) - 3059349..3059522 (+) 174 WP_034126248.1 DUF3094 family protein -
  FXO12_RS13810 (FXO12_13810) - 3059583..3060881 (+) 1299 WP_032903990.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43701.36 Da        Isoelectric Point: 10.1291

>NTDB_id=380428 FXO12_RS13770 WP_057976748.1 3054682..3055890(+) (pilC) [Pseudomonas sp. J380]
MNDTPTIYAWEGINRKGRRVSGQIAGHNLALIKVQLRQQGICPERVRKKSCGLHIFKTRITPADIALFTRQLATLLKAGI
PLLQAFDIISEGVENLQLRELVKALKQHIASGHPLAAALRQYPRYFDELYCNLVAAGEIAGALETLLERVAIHLEKSQRL
KTRIKKAMTYPVAVLVIATGVSTVLLIHVVPQFQNLFAGVNGQLPGFTLAVIALSELLQHAWWMLALAVGAIMVAGHHAY
RTYSGFRHWLAAGLLKVPVAGKLLHRSAVARYARTLSTTFAAGVPLVQALGSVAGAVGNGPFKQAIERMRHDVSTGMQLN
QSMHNSGLFPGMAIQMTAIGEESGTLDRMLEKVANHYESDVDTLVDNLTSLMEPLIMVVLGGIVGALVVAMYLPVFQLGT
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=380428 FXO12_RS13770 WP_057976748.1 3054682..3055890(+) (pilC) [Pseudomonas sp. J380]
ATGAACGACACTCCAACAATCTATGCCTGGGAAGGCATCAACCGCAAAGGCCGCCGGGTTTCCGGGCAAATCGCCGGGCA
TAACCTTGCGCTGATCAAGGTACAACTGCGCCAGCAAGGGATCTGCCCTGAGCGTGTGCGCAAGAAATCTTGCGGTTTAC
ACATCTTCAAGACGCGAATAACGCCCGCAGATATTGCCCTGTTCACTCGCCAGTTAGCCACCTTGCTCAAAGCCGGCATC
CCGCTGCTGCAAGCCTTCGACATCATCAGCGAAGGCGTCGAAAACCTACAGCTGCGCGAACTGGTGAAGGCGTTGAAACA
GCACATTGCCAGCGGCCACCCCTTGGCCGCTGCCCTGCGTCAATATCCCCGCTACTTCGATGAGTTGTATTGCAATCTGG
TAGCAGCTGGCGAAATAGCCGGCGCCCTGGAAACCTTGCTCGAGCGCGTGGCGATTCATCTGGAAAAAAGCCAACGGCTC
AAAACCAGGATCAAGAAAGCCATGACCTACCCTGTCGCGGTACTGGTAATTGCCACAGGGGTGAGCACAGTCCTGTTGAT
TCACGTTGTGCCGCAGTTCCAGAACTTGTTCGCCGGGGTCAACGGCCAATTGCCGGGGTTTACCCTGGCGGTCATTGCGC
TGTCGGAACTGTTGCAACACGCGTGGTGGATGCTGGCGCTGGCCGTGGGCGCCATAATGGTCGCTGGGCATCACGCTTAT
CGAACTTACTCGGGCTTTCGCCATTGGTTGGCGGCAGGTTTGTTGAAAGTACCCGTGGCAGGCAAACTGCTGCACAGATC
TGCGGTTGCTCGCTACGCTCGCACACTTTCCACCACCTTTGCGGCGGGCGTCCCTCTGGTACAGGCATTGGGTTCGGTGG
CCGGCGCCGTCGGCAATGGCCCCTTCAAACAGGCAATCGAACGTATGCGCCACGATGTATCCACAGGCATGCAGCTGAAT
CAATCGATGCATAACAGCGGCCTGTTCCCAGGCATGGCGATCCAGATGACCGCAATCGGGGAGGAGTCCGGCACCCTGGA
CCGCATGCTGGAAAAAGTCGCGAACCACTACGAGTCAGACGTGGATACCTTGGTCGACAACCTCACCAGCCTGATGGAGC
CGCTGATCATGGTCGTATTGGGGGGGATTGTCGGCGCGCTGGTAGTCGCCATGTACCTGCCGGTGTTTCAATTGGGGACG
GCGTTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Q0HS76

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.683

98.756

0.57
  pilC Acinetobacter baumannii D1279779

50.503

99.005

0.5
  pilC Acinetobacter baylyi ADP1

50.505

98.507

0.498
  pilC Legionella pneumophila strain ERS1305867

48.507

100

0.485
  pilC Vibrio cholerae strain A1552

39.848

98.01

0.391
  pilG Neisseria gonorrhoeae MS11

40.519

95.771

0.388
  pilC Thermus thermophilus HB27

38.404

99.751

0.383
  pilG Neisseria meningitidis 44/76-A

39.583

95.522

0.378
  pilC Vibrio campbellii strain DS40M4

37.245

97.512

0.363

Multiple sequence alignment