Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FR741_RS03125 Genome accession   NZ_CP042451
Coordinates   631863..633548 (-) Length   561 a.a.
NCBI ID   WP_167426248.1    Uniprot ID   -
Organism   Vibrio diabolicus strain FA3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 626863..638548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FR741_RS03095 (FR741_03240) rplS 627103..627456 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  FR741_RS03100 (FR741_03245) yacG 627946..628140 (-) 195 WP_005396692.1 DNA gyrase inhibitor YacG -
  FR741_RS03105 (FR741_03250) zapD 628299..629039 (-) 741 WP_012841416.1 cell division protein ZapD -
  FR741_RS03110 (FR741_03255) coaE 629067..629681 (-) 615 WP_047008979.1 dephospho-CoA kinase -
  FR741_RS03115 (FR741_03260) pilD 629682..630551 (-) 870 WP_012841418.1 A24 family peptidase Machinery gene
  FR741_RS03120 (FR741_03265) pilC 630616..631839 (-) 1224 WP_017635918.1 type II secretion system F family protein Machinery gene
  FR741_RS03125 (FR741_03270) pilB 631863..633548 (-) 1686 WP_167426248.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FR741_RS03130 (FR741_03275) pilA 633548..634027 (-) 480 WP_167426249.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  FR741_RS03135 (FR741_03285) nadC 634292..635179 (-) 888 WP_167426250.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FR741_RS03140 (FR741_03290) ampD 635272..635823 (+) 552 WP_046875077.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FR741_RS03145 (FR741_03295) pdhR 636229..636996 (+) 768 WP_005396680.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62184.04 Da        Isoelectric Point: 5.4048

>NTDB_id=377992 FR741_RS03125 WP_167426248.1 631863..633548(-) (pilB) [Vibrio diabolicus strain FA3]
MLTNLSTVLRQKGLLTFSQEESLIEQVKASGISMPEALLSSGLLTSSELAEHLSSLFSLSQPELSQYEYASLCQQLGLRE
LITRHNALPLQRTPSTLLLAVADPTNQQTEDDFRFATGLQVELVLADFRQLSAAIRRLYGRSLSHEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKQSQELTVQLQHLGIQASDN
IFKADPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=377992 FR741_RS03125 WP_167426248.1 631863..633548(-) (pilB) [Vibrio diabolicus strain FA3]
ATGCTAACCAACCTCTCAACAGTTCTTCGCCAAAAAGGGTTACTGACCTTTTCCCAAGAAGAATCGTTAATAGAGCAAGT
CAAAGCTTCTGGCATTTCGATGCCAGAGGCTTTACTCAGTTCTGGATTATTGACATCAAGCGAACTCGCTGAGCACTTAA
GTTCTCTCTTTAGTTTGAGTCAGCCCGAGTTATCTCAGTATGAGTACGCTTCGCTTTGTCAACAACTCGGTCTACGTGAA
TTAATCACTCGACATAACGCACTCCCACTCCAGCGTACGCCTTCAACTTTGTTATTAGCCGTTGCCGACCCGACGAACCA
ACAAACAGAAGATGACTTTCGTTTTGCGACAGGGTTACAGGTTGAACTAGTCTTGGCGGATTTTCGTCAACTCAGCGCGG
CAATTCGACGCTTGTATGGTCGTTCATTGAGTCATGAGAAGTCTGGATTAAAAGAGATCAACCAAGAAGAGTTAGCCAAT
CTGGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATAAA
CCAAATTCTGTTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAACCTTATGAAAAGATGTACCGCGTTCGCC
TACGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTAAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCGAT
TGATATGCGTGTATCCACGCTACCGACTCTATTCGGAGAGAAAATTGTATTACGACTGCTCGACAGCAGCTCTGCGTCAC
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGCCCTACCGGGAGCGGAAAAACCGTTTCTCTGTACACTGGGCTGAGTATTCTCAATAAGCCAGAAATCAA
CATTTCCACAGCAGAAGATCCGGTAGAAATTAACTTATCGGGCATTAACCAAGTACAAGTACAACCCAAGATCGGCTTTG
GCTTCGCAGAGGCATTGCGCTCTTTTCTTCGTCAAGACCCCGATGTGGTAATGGTCGGTGAGATTCGTGACTTAGACACT
GCGGAAATCGCCATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTACATACCAATTCTGCGGCTGAAACGGT
GATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCCTCTCTTAGTTTGATTATTGCCCAGCGCTTGGCCC
GTAAACTGTGCTCGCACTGCAAACAATCACAAGAGCTTACCGTTCAGCTACAACATCTCGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCGATCCCGATGGATGCAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCGGAAGCGCTTATTAAAGGCGCCTCGGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

95.544

100

0.955
  pilB Vibrio campbellii strain DS40M4

89.84

100

0.898
  pilB Vibrio cholerae strain A1552

73.843

100

0.74
  pilB Acinetobacter baumannii D1279779

51.619

93.583

0.483
  pilB Legionella pneumophila strain ERS1305867

49.353

96.435

0.476
  pilB Acinetobacter baylyi ADP1

47.217

99.287

0.469
  pilF Neisseria gonorrhoeae MS11

45.213

100

0.455
  pilB/pilB1 Synechocystis sp. PCC 6803

37.037

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.508

100

0.369
  pilF Thermus thermophilus HB27

35.943

100

0.36

Multiple sequence alignment