Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FR741_RS03130 Genome accession   NZ_CP042451
Coordinates   633548..634027 (-) Length   159 a.a.
NCBI ID   WP_167426249.1    Uniprot ID   -
Organism   Vibrio diabolicus strain FA3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 628548..639027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FR741_RS03110 (FR741_03255) coaE 629067..629681 (-) 615 WP_047008979.1 dephospho-CoA kinase -
  FR741_RS03115 (FR741_03260) pilD 629682..630551 (-) 870 WP_012841418.1 A24 family peptidase Machinery gene
  FR741_RS03120 (FR741_03265) pilC 630616..631839 (-) 1224 WP_017635918.1 type II secretion system F family protein Machinery gene
  FR741_RS03125 (FR741_03270) pilB 631863..633548 (-) 1686 WP_167426248.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FR741_RS03130 (FR741_03275) pilA 633548..634027 (-) 480 WP_167426249.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  FR741_RS03135 (FR741_03285) nadC 634292..635179 (-) 888 WP_167426250.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FR741_RS03140 (FR741_03290) ampD 635272..635823 (+) 552 WP_046875077.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FR741_RS03145 (FR741_03295) pdhR 636229..636996 (+) 768 WP_005396680.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16931.04 Da        Isoelectric Point: 4.7159

>NTDB_id=377993 FR741_RS03130 WP_167426249.1 633548..634027(-) (pilA) [Vibrio diabolicus strain FA3]
MKTNKQKKQQGFTLIELMIVVAIIGVLAAYAIPAYQDYTKRATLGEFPKAASSVKVAVELCGHENAADSDSFKDSCVGGT
DGNNGVPGTFELNLMEITTAEGSASGAVDIRVKATDAKGPIKQNETYVLTGTYSQNGITWSGKCYTDAAMQNEQTDYCP

Nucleotide


Download         Length: 480 bp        

>NTDB_id=377993 FR741_RS03130 WP_167426249.1 633548..634027(-) (pilA) [Vibrio diabolicus strain FA3]
ATGAAAACGAATAAACAGAAGAAGCAGCAAGGTTTTACGCTGATTGAATTAATGATTGTGGTAGCGATTATCGGTGTTTT
AGCAGCATATGCAATCCCTGCTTACCAAGACTATACAAAACGTGCGACTCTCGGAGAGTTCCCCAAAGCTGCATCTTCCG
TAAAGGTAGCAGTTGAATTATGTGGCCATGAAAATGCAGCAGATTCTGACTCATTTAAAGATTCCTGTGTAGGTGGTACT
GATGGAAACAATGGAGTTCCTGGCACATTTGAACTAAACCTTATGGAAATTACGACGGCTGAAGGTAGCGCATCAGGAGC
GGTAGATATCCGAGTTAAAGCTACTGATGCCAAAGGGCCAATTAAACAAAATGAAACTTATGTATTAACTGGAACCTACT
CCCAAAATGGTATTACATGGTCAGGCAAGTGTTATACCGATGCCGCTATGCAAAACGAACAAACAGATTACTGCCCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

64.151

100

0.642
  pilA Haemophilus influenzae 86-028NP

37.821

98.113

0.371
  pilA Haemophilus influenzae Rd KW20

37.179

98.113

0.365

Multiple sequence alignment