Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   FGL73_RS08115 Genome accession   NZ_CP042408
Coordinates   1683896..1684834 (-) Length   312 a.a.
NCBI ID   WP_058205111.1    Uniprot ID   -
Organism   Lactococcus lactis strain CBA3619     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1678896..1689834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL73_RS08075 (FGL73_08075) - 1679784..1680626 (-) 843 WP_038602352.1 metal ABC transporter substrate-binding protein -
  FGL73_RS08080 (FGL73_08080) - 1680623..1681060 (-) 438 WP_021721874.1 zinc-dependent MarR family transcriptional regulator -
  FGL73_RS08085 (FGL73_08085) comGG 1681141..1681416 (-) 276 WP_021721873.1 hypothetical protein Machinery gene
  FGL73_RS08090 (FGL73_08090) comGF 1681464..1681910 (-) 447 WP_021721872.1 competence type IV pilus minor pilin ComGF Machinery gene
  FGL73_RS08095 (FGL73_08095) comGE 1681873..1682169 (-) 297 WP_038602356.1 competence type IV pilus minor pilin ComGE Machinery gene
  FGL73_RS08100 (FGL73_08100) comGD 1682141..1682572 (-) 432 WP_080651959.1 competence type IV pilus minor pilin ComGD Machinery gene
  FGL73_RS08105 (FGL73_08105) comGC 1682532..1682915 (-) 384 WP_080739889.1 competence type IV pilus major pilin ComGC Machinery gene
  FGL73_RS08110 (FGL73_08110) comGB 1682929..1684002 (-) 1074 WP_080739890.1 competence type IV pilus assembly protein ComGB Machinery gene
  FGL73_RS08115 (FGL73_08115) comGA 1683896..1684834 (-) 939 WP_058205111.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35579.84 Da        Isoelectric Point: 6.4008

>NTDB_id=377723 FGL73_RS08115 WP_058205111.1 1683896..1684834(-) (comGA) [Lactococcus lactis strain CBA3619]
MIPKMAKELIQKAIDRGASDIYLIASKEKYKLYFRQMTARVLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHAASVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTSTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=377723 FGL73_RS08115 WP_058205111.1 1683896..1684834(-) (comGA) [Lactococcus lactis strain CBA3619]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAAAGCAATTGATAGAGGTGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCGCGTGTTCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
TTAGAGGAAAGTAATGCAAAACGTTTACGTCTTTCAACAGTGGGGGATTTCGAGGGTAATGAATCCTTGGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCTGGTCCAGTAGGCTCGGGCAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTATTTTCAACCGTTCATGCCGCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGATATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACATCTACTGAAGCCAAGAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

77.885

100

0.779
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.984

99.679

0.548
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

54.019

99.679

0.538
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.055

99.679

0.529
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.055

99.679

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.055

99.679

0.529
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.055

99.679

0.529
  comYA Streptococcus mutans UA140

52.244

100

0.522
  comYA Streptococcus mutans UA159

52.244

100

0.522
  comYA Streptococcus gordonii str. Challis substr. CH1

51.438

100

0.516

Multiple sequence alignment