Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   FOZ74_RS02785 Genome accession   NZ_CP042344
Coordinates   548122..549339 (+) Length   405 a.a.
NCBI ID   WP_146911643.1    Uniprot ID   A0A5B8RQZ2
Organism   Comamonas flocculans strain NLF-7-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 543122..554339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOZ74_RS02760 (FOZ74_02760) - 543262..543624 (-) 363 WP_146911638.1 DsrE family protein -
  FOZ74_RS02765 (FOZ74_02765) - 543715..545478 (-) 1764 WP_146911639.1 methyl-accepting chemotaxis protein -
  FOZ74_RS02770 (FOZ74_02770) rpmB 545750..545983 (+) 234 WP_146911640.1 50S ribosomal protein L28 -
  FOZ74_RS02775 (FOZ74_02775) rpmG 546003..546173 (+) 171 WP_146911641.1 50S ribosomal protein L33 -
  FOZ74_RS02780 (FOZ74_02780) pilB 546362..548095 (+) 1734 WP_146911642.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FOZ74_RS02785 (FOZ74_02785) pilC 548122..549339 (+) 1218 WP_146911643.1 type II secretion system F family protein Machinery gene
  FOZ74_RS02790 (FOZ74_02790) pilD 549343..550236 (+) 894 WP_146911644.1 A24 family peptidase Machinery gene
  FOZ74_RS02795 (FOZ74_02795) coaE 550243..550860 (+) 618 WP_146911645.1 dephospho-CoA kinase -
  FOZ74_RS02800 (FOZ74_02800) zapD 550890..551645 (+) 756 WP_146911646.1 cell division protein ZapD -
  FOZ74_RS02805 (FOZ74_02805) - 551823..552110 (+) 288 WP_146911647.1 DUF1330 domain-containing protein -
  FOZ74_RS02810 (FOZ74_02810) - 552253..552816 (+) 564 WP_146911648.1 DUF6622 family protein -
  FOZ74_RS02815 (FOZ74_02815) - 552830..553117 (+) 288 WP_146911649.1 2TM domain-containing protein -
  FOZ74_RS02820 (FOZ74_02820) - 553124..553783 (+) 660 WP_146911650.1 glutathione S-transferase -
  FOZ74_RS02825 (FOZ74_02825) - 553780..554262 (+) 483 WP_146911651.1 DUF4188 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44470.37 Da        Isoelectric Point: 9.4891

>NTDB_id=377177 FOZ74_RS02785 WP_146911643.1 548122..549339(+) (pilC) [Comamonas flocculans strain NLF-7-7]
MATAAAKGIKDFVFEWEGKDRNGKIVRGELRAGGENQVQASLRRQGVMPTKIKKRRMRSGKKIKPKDIALFTRQMATMMK
AGVPLLQAFDIVGRGNTNPSVTKLLNDIRNDVETGTSLNAAFRKYPMYFDSLYCNLVEAGEAAGILEALLDRLATYMEKT
EAIKSKIKSALMYPISVIIVAFVVVTVIMIFVIPAFKEVFTSFGADLPAPTLIVMGMSEFFVQYWWLIFGVLGGGFYFFM
QAWRRSEKMQMAMDRFLLKIPIFGDLINKSAVARWTRTLSTMFAAGVPLVEALDSVGGAAGNAVYAVATDKIQQEVSTGT
SLTAAMTNANVFPSMVLQMCAIGEESGSIDHMLGKAADFYEDEVDEMVKGLSSLLEPIIIVFLGTIIGGIVVSMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=377177 FOZ74_RS02785 WP_146911643.1 548122..549339(+) (pilC) [Comamonas flocculans strain NLF-7-7]
ATGGCAACCGCTGCGGCCAAAGGCATCAAGGACTTCGTATTCGAATGGGAGGGCAAGGACCGCAACGGAAAGATCGTGCG
CGGCGAGCTGCGTGCCGGTGGCGAGAACCAGGTCCAGGCCAGCCTGCGCCGCCAGGGGGTCATGCCCACCAAGATCAAGA
AGCGGCGCATGCGCTCGGGCAAGAAGATCAAGCCCAAGGACATCGCCCTGTTCACGCGCCAGATGGCCACCATGATGAAG
GCCGGCGTGCCGCTGCTGCAGGCCTTCGACATCGTCGGGCGCGGCAACACCAATCCGAGCGTGACCAAGCTGCTCAACGA
CATCCGCAACGACGTCGAAACCGGCACCTCGCTGAACGCGGCTTTTCGCAAGTACCCGATGTATTTCGACAGCCTGTACT
GCAACCTGGTGGAGGCCGGCGAGGCTGCCGGCATCCTGGAGGCGCTGCTCGACCGGCTGGCCACCTACATGGAAAAGACC
GAGGCCATCAAGAGCAAGATCAAGTCGGCGCTGATGTACCCGATCTCGGTGATCATCGTCGCCTTCGTCGTGGTCACGGT
GATCATGATCTTCGTCATCCCTGCCTTCAAGGAGGTGTTCACCTCCTTCGGCGCCGACCTGCCCGCGCCCACGCTCATCG
TGATGGGCATGAGCGAATTCTTCGTGCAGTACTGGTGGCTGATCTTCGGCGTGCTGGGCGGGGGCTTCTACTTCTTCATG
CAGGCCTGGCGGCGCAGCGAGAAGATGCAGATGGCGATGGACCGCTTCCTGCTCAAGATCCCGATCTTCGGCGACCTGAT
CAACAAGTCGGCGGTGGCGCGCTGGACGCGCACGCTCTCGACCATGTTCGCCGCCGGCGTGCCGCTGGTGGAAGCGCTCG
ATTCGGTCGGCGGCGCCGCAGGCAACGCGGTCTATGCGGTGGCCACCGACAAGATCCAGCAGGAAGTCTCCACCGGCACC
AGCCTCACCGCCGCCATGACCAATGCCAACGTCTTCCCTTCCATGGTGCTGCAGATGTGCGCCATCGGTGAGGAATCGGG
CTCGATCGACCACATGCTGGGCAAGGCGGCGGATTTCTACGAGGACGAGGTCGACGAAATGGTCAAGGGCCTGTCCAGCC
TGCTCGAGCCCATCATCATCGTCTTCCTGGGCACCATCATCGGCGGCATCGTGGTGTCCATGTACCTGCCCATCTTCAAG
CTCGGCCAGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8RQZ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.198

99.753

0.551
  pilG Neisseria gonorrhoeae MS11

53.5

98.765

0.528
  pilG Neisseria meningitidis 44/76-A

53.25

98.765

0.526
  pilC Legionella pneumophila strain ERS1305867

50.754

98.272

0.499
  pilC Acinetobacter baylyi ADP1

50

98.272

0.491
  pilC Acinetobacter baumannii D1279779

48.615

98.025

0.477
  pilC Vibrio cholerae strain A1552

40.554

98.025

0.398
  pilC Vibrio campbellii strain DS40M4

39

98.765

0.385

Multiple sequence alignment