Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FOZ74_RS02780 Genome accession   NZ_CP042344
Coordinates   546362..548095 (+) Length   577 a.a.
NCBI ID   WP_146911642.1    Uniprot ID   A0A5B8RT87
Organism   Comamonas flocculans strain NLF-7-7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 541362..553095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOZ74_RS02755 (FOZ74_02755) - 542779..543237 (+) 459 WP_146911637.1 RidA family protein -
  FOZ74_RS02760 (FOZ74_02760) - 543262..543624 (-) 363 WP_146911638.1 DsrE family protein -
  FOZ74_RS02765 (FOZ74_02765) - 543715..545478 (-) 1764 WP_146911639.1 methyl-accepting chemotaxis protein -
  FOZ74_RS02770 (FOZ74_02770) rpmB 545750..545983 (+) 234 WP_146911640.1 50S ribosomal protein L28 -
  FOZ74_RS02775 (FOZ74_02775) rpmG 546003..546173 (+) 171 WP_146911641.1 50S ribosomal protein L33 -
  FOZ74_RS02780 (FOZ74_02780) pilB 546362..548095 (+) 1734 WP_146911642.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FOZ74_RS02785 (FOZ74_02785) pilC 548122..549339 (+) 1218 WP_146911643.1 type II secretion system F family protein Machinery gene
  FOZ74_RS02790 (FOZ74_02790) pilD 549343..550236 (+) 894 WP_146911644.1 A24 family peptidase Machinery gene
  FOZ74_RS02795 (FOZ74_02795) coaE 550243..550860 (+) 618 WP_146911645.1 dephospho-CoA kinase -
  FOZ74_RS02800 (FOZ74_02800) zapD 550890..551645 (+) 756 WP_146911646.1 cell division protein ZapD -
  FOZ74_RS02805 (FOZ74_02805) - 551823..552110 (+) 288 WP_146911647.1 DUF1330 domain-containing protein -
  FOZ74_RS02810 (FOZ74_02810) - 552253..552816 (+) 564 WP_146911648.1 DUF6622 family protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62583.58 Da        Isoelectric Point: 5.1526

>NTDB_id=377176 FOZ74_RS02780 WP_146911642.1 546362..548095(+) (pilB) [Comamonas flocculans strain NLF-7-7]
MATAGTAPKEAAAVALPSLARALMSAGKLAQNSAEDISRKAQASRNSFIAELLAAKSLSAGELAQTLSNTFGAPLLDVDA
LDAAHLPRDLLDTKLCQAYKVVALAKRSNRLIALTADPTNQEAAEKIKFTTQLGVDWVVGEYDKLLRLVEATTKSVSESL
DSITGGDFEFDDAPVEEETEGPDTAGADVEDAPVVRFLHKMLLDAFNMRASDLHFEPYEHNYRVRFRVDGELQEITSPPV
AIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARMGIDALGYEPEEKER
LLAAINRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNISTAEDPSEINMPGVNQVNVNEKAGLTFAVALKAFLRQDPD
IIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKQPADIPH
ESLVEAGYREEDIDGSWVTYKPVGCAACNNGYKGRVGIYEVMPISEELQRIILADGSALEIAAQARAEGVRSLRQSGLHK
ARMGFTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=377176 FOZ74_RS02780 WP_146911642.1 546362..548095(+) (pilB) [Comamonas flocculans strain NLF-7-7]
ATGGCCACCGCCGGTACCGCACCAAAGGAAGCAGCAGCCGTGGCATTGCCCAGCCTGGCGCGCGCGCTCATGTCGGCAGG
CAAGCTGGCGCAGAACAGCGCCGAAGACATCTCGCGCAAGGCACAGGCAAGCCGCAACAGCTTCATTGCGGAGCTGCTCG
CAGCCAAGTCCCTGAGCGCCGGCGAGCTGGCGCAGACGCTGTCCAACACCTTTGGTGCGCCCCTGCTGGACGTGGATGCG
CTGGACGCCGCCCACCTGCCCCGGGACCTGCTCGACACCAAGCTGTGCCAGGCCTACAAGGTCGTGGCCCTGGCCAAGCG
CAGCAACCGGCTGATCGCGCTCACCGCCGACCCGACCAACCAGGAGGCGGCAGAGAAGATCAAGTTCACCACCCAGCTCG
GCGTGGACTGGGTGGTGGGCGAATACGACAAGCTGCTGCGGCTGGTCGAGGCGACGACCAAGAGCGTCTCCGAGAGCCTG
GACAGCATCACCGGCGGCGACTTCGAGTTCGACGACGCCCCGGTCGAGGAAGAAACCGAAGGCCCGGACACCGCGGGCGC
CGACGTCGAGGACGCGCCGGTCGTGCGCTTCCTGCACAAGATGCTGCTGGACGCGTTCAACATGCGCGCCTCCGACCTGC
ACTTCGAGCCCTACGAGCACAACTACCGGGTGCGTTTTCGCGTCGACGGCGAGTTGCAGGAGATCACCTCCCCGCCGGTG
GCCATCAAGGACAAGCTCGCGTCGCGCATCAAGGTGATCTCGCGCCTCGATATCTCGGAAAAGCGCGTGCCCCAGGACGG
GCGCATGAAGCTCAAGGTCGGGCCGGACCGCGTCATCGACTTTCGCGTCAGCACGCTGCCGACGCTGTTCGGCGAAAAGA
TCGTCATCCGCATCCTGGACCCGAGCAGCGCGCGCATGGGCATCGACGCGCTGGGCTACGAGCCCGAGGAGAAGGAGCGG
CTGCTGGCCGCGATCAACCGCCCCTACGGCATGATCCTGGTGACCGGCCCCACGGGCTCGGGCAAGACGGTCTCGCTCTA
CACCTGCCTGAATCTGCTGAACAAGCCGGGCGTGAACATCTCCACCGCCGAAGACCCCTCGGAAATCAACATGCCGGGCG
TCAACCAGGTCAACGTCAACGAAAAGGCGGGGCTGACCTTCGCGGTGGCACTCAAGGCCTTCCTGCGCCAGGACCCGGAC
ATCATCATGGTGGGCGAAATCCGCGACCTGGAAACCGCCGACATCGCCATCAAGGCCGCGCAGACCGGCCACCTGGTGCT
CTCGACGCTGCACACCAACGACGCGCCGACCACGCTCACGCGCATGCGCAACATGGGCATCGCGCCGTTCAACATCGCCT
CCAGCGTGATCCTGATCACCGCCCAGCGCCTGGCGCGGCGCCTGTGCCCGCAGTGCAAGCAGCCGGCCGACATCCCGCAT
GAAAGCCTGGTCGAGGCAGGCTACCGGGAAGAAGACATCGACGGCAGCTGGGTCACCTACAAGCCCGTGGGCTGCGCCGC
CTGCAACAACGGCTACAAGGGGCGGGTCGGCATCTATGAAGTGATGCCGATCTCCGAGGAGCTGCAGCGCATCATCCTGG
CCGACGGCAGCGCGCTGGAGATTGCCGCCCAGGCACGCGCCGAAGGCGTGCGCTCGCTGCGCCAATCGGGCCTGCACAAG
GCCAGGATGGGCTTCACCTCGCTCGAAGAGGTGCTGGCAGTCACCAACGAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.345

100

0.539
  pilB Acinetobacter baumannii D1279779

55.433

94.107

0.522
  pilF Neisseria gonorrhoeae MS11

51.675

98.267

0.508
  pilB Legionella pneumophila strain ERS1305867

49.647

98.094

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

46.82

98.094

0.459
  pilB Vibrio cholerae strain A1552

46.06

98.96

0.456
  pilB Vibrio campbellii strain DS40M4

46.466

98.094

0.456
  pilF Thermus thermophilus HB27

39.531

96.014

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.824

92.548

0.378

Multiple sequence alignment