Detailed information NTG0377178

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FOZ74_RS02790
• Genome accession: NZ_CP042344
• Coordinates: 549343..550236 (+)
• Length: 297 a.a.
• NCBI ID: WP_146911644.1
• Uniprot ID: A0A5B8RQY6
• Organism: Comamonas flocculans strain NLF-7-7
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 544343..555236

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FOZ74_RS02770 (FOZ74_02770)	rpmB	545750..545983 (+)	234	WP_146911640.1	50S ribosomal protein L28	-
FOZ74_RS02775 (FOZ74_02775)	rpmG	546003..546173 (+)	171	WP_146911641.1	50S ribosomal protein L33	-
FOZ74_RS02780 (FOZ74_02780)	pilB	546362..548095 (+)	1734	WP_146911642.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FOZ74_RS02785 (FOZ74_02785)	pilC	548122..549339 (+)	1218	WP_146911643.1	type II secretion system F family protein	Machinery gene
FOZ74_RS02790 (FOZ74_02790)	pilD	549343..550236 (+)	894	WP_146911644.1	A24 family peptidase	Machinery gene
FOZ74_RS02795 (FOZ74_02795)	coaE	550243..550860 (+)	618	WP_146911645.1	dephospho-CoA kinase	-
FOZ74_RS02800 (FOZ74_02800)	zapD	550890..551645 (+)	756	WP_146911646.1	cell division protein ZapD	-
FOZ74_RS02805 (FOZ74_02805)	-	551823..552110 (+)	288	WP_146911647.1	DUF1330 domain-containing protein	-
FOZ74_RS02810 (FOZ74_02810)	-	552253..552816 (+)	564	WP_146911648.1	DUF6622 family protein	-
FOZ74_RS02815 (FOZ74_02815)	-	552830..553117 (+)	288	WP_146911649.1	2TM domain-containing protein	-
FOZ74_RS02820 (FOZ74_02820)	-	553124..553783 (+)	660	WP_146911650.1	glutathione S-transferase	-
FOZ74_RS02825 (FOZ74_02825)	-	553780..554262 (+)	483	WP_146911651.1	DUF4188 domain-containing protein	-

Sequence

Protein

• Length: 297 a.a.
• Molecular weight: 31351.79 Da
• Isoelectric Point: 7.1591

>NTDB_id=377178 FOZ74_RS02790 WP_146911644.1 549343..550236(+) (pilD) [Comamonas flocculans strain NLF-7-7]
MTASVWTDAALLGVLGLLIGSFLNVVIHRLPRMMHHQWAQECAAWAAEQNGGGSGPESAPTGEPAPYNLLVPRSQCPHCA
HPIRWFENIPVFSYLALRGRCAGCGKAIGLRYPAVELATGLLFFFCGLHWGLGPAGLAWSAFSAALVAAACIDWDTTLLP
DDITLPLLWGGLLASLLHYSGVTLTDAVLGAMGGYLSLWSVYWGFKLATGKEGMGYGDFKLFAALGAWFGWQALVPIILL
ASVVGAVVGVVLKLRGGLHEGKYVPFGPFLAGGGFVALLWGPQQVMHALLGVLGLAA

Nucleotide

• Length: 894 bp

>NTDB_id=377178 FOZ74_RS02790 WP_146911644.1 549343..550236(+) (pilD) [Comamonas flocculans strain NLF-7-7]
ATGACGGCGTCGGTCTGGACGGACGCCGCGCTGCTCGGCGTCCTGGGGCTGCTGATCGGCAGCTTTCTGAACGTGGTGAT
CCACCGCCTGCCGCGCATGATGCACCACCAGTGGGCGCAGGAGTGCGCGGCCTGGGCCGCGGAGCAGAACGGCGGCGGCT
CCGGCCCGGAGTCCGCACCAACGGGCGAACCCGCTCCCTACAACCTGCTGGTGCCGCGCTCGCAGTGTCCGCATTGCGCG
CACCCGATACGCTGGTTCGAGAATATTCCCGTCTTCAGCTATCTGGCGCTGCGCGGGCGCTGCGCGGGCTGCGGCAAGGC
CATCGGCCTGCGCTATCCGGCGGTGGAGCTCGCCACCGGGCTGCTGTTCTTCTTCTGCGGGCTGCACTGGGGCCTGGGCC
CGGCGGGCCTGGCATGGAGCGCCTTCTCGGCCGCGCTGGTGGCCGCTGCCTGCATAGACTGGGACACCACGCTGCTGCCC
GACGACATCACGCTGCCGCTGCTCTGGGGCGGGCTGCTCGCGAGCCTGCTGCACTACAGCGGCGTCACGCTCACCGACGC
GGTGCTGGGCGCCATGGGTGGCTACCTGTCGCTGTGGAGCGTCTACTGGGGCTTCAAGCTTGCCACCGGCAAGGAAGGCA
TGGGCTACGGCGACTTCAAGCTGTTTGCCGCGCTGGGCGCATGGTTCGGCTGGCAGGCGCTGGTGCCGATCATCCTGCTG
GCCTCGGTCGTCGGTGCGGTCGTGGGCGTGGTGCTCAAGCTCAGGGGCGGGCTGCACGAGGGCAAGTACGTGCCCTTCGG
CCCGTTTCTGGCCGGTGGCGGTTTCGTGGCCCTGTTGTGGGGCCCGCAGCAGGTCATGCACGCTCTGCTCGGCGTGCTGG
GCCTGGCGGCCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (143-250)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (14-129)

3D structure

• AlphaFold DB: A0A5B8RQY6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.786	94.276	0.488
pilD	Vibrio campbellii strain DS40M4	48.454	97.98	0.475
pilD	Acinetobacter baumannii D1279779	44.484	94.613	0.421
pilD	Acinetobacter nosocomialis M2	44.484	94.613	0.421
pilD	Neisseria gonorrhoeae MS11	44.361	89.562	0.397