Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   M301_RS03095 Genome accession   NC_014207
Coordinates   661926..663143 (-) Length   405 a.a.
NCBI ID   WP_013147302.1    Uniprot ID   D7DN47
Organism   Methylotenera versatilis 301     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 656926..668143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M301_RS03075 (M301_0598) - 657295..658617 (+) 1323 WP_013147298.1 aminopeptidase P N-terminal domain-containing protein -
  M301_RS03080 (M301_0599) - 658631..659800 (+) 1170 WP_013147299.1 FAD-dependent oxidoreductase -
  M301_RS03085 (M301_0600) - 659797..661005 (+) 1209 WP_013147300.1 FAD-dependent monooxygenase -
  M301_RS03090 (M301_0601) - 661079..661807 (+) 729 WP_013147301.1 DsbC family protein -
  M301_RS03095 (M301_0602) pilC 661926..663143 (-) 1218 WP_013147302.1 type II secretion system F family protein Machinery gene
  M301_RS03100 (M301_0603) pilB 663158..664876 (-) 1719 WP_013147303.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M301_RS03105 (M301_0604) - 665214..665417 (+) 204 WP_013147304.1 cold-shock protein -
  M301_RS03110 (M301_0605) icd 665526..666782 (-) 1257 WP_013147305.1 NADP-dependent isocitrate dehydrogenase -
  M301_RS03115 (M301_0606) - 666828..667379 (+) 552 WP_013147306.1 pseudouridine synthase -
  M301_RS03120 (M301_0607) - 667407..668117 (+) 711 WP_041359733.1 type 1 glutamine amidotransferase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44110.01 Da        Isoelectric Point: 9.7378

>NTDB_id=37381 M301_RS03095 WP_013147302.1 661926..663143(-) (pilC) [Methylotenera versatilis 301]
MAANIKATKEVTYSWEGKDKKGRSVKGEIRATGESFVNATLRRQGIVVTKVKKQSSFTNKGSVSDKDITLFTRQLATMMK
AGVPLLQSFDIVGKGHSNPAVAKLLLDIKSDVETGSSMSAAFRKYPLYFDALFCNLIGAGEQAGILDTLLDRLATYKEKI
LAIKSKIKSALFYPISIIVVAFVIVAVIMIFVIPAFKTLFDGFGAELPAPTLIVMAISDIFVQWWWAIFGSIGFGIWFFF
YTWKRSLSMQATMDRLILKVPVFGPLIRKATIARWARTLATMFSAGVPLVEALDSVAGASGNRVYYDATKKVQSEISTGT
SLTVAMQNTDVFPNMVLQMTAIGEESGALDAMLSKVADFFEAEVDDAVDALASLMEPVIMVVLGVLIGGLVIAMYMPIFK
MGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=37381 M301_RS03095 WP_013147302.1 661926..663143(-) (pilC) [Methylotenera versatilis 301]
ATGGCAGCAAATATTAAAGCCACCAAAGAAGTTACATACTCTTGGGAAGGTAAAGACAAAAAGGGCCGGTCAGTCAAAGG
CGAAATACGAGCCACAGGTGAGTCTTTTGTGAATGCCACTTTGCGACGTCAGGGCATTGTTGTGACCAAAGTAAAAAAAC
AAAGCAGCTTCACGAATAAAGGTAGCGTTTCAGATAAAGACATTACGCTTTTTACTCGCCAACTTGCTACCATGATGAAA
GCAGGCGTACCACTTCTACAATCTTTTGATATTGTAGGTAAAGGCCATAGTAATCCAGCTGTCGCTAAGCTTTTATTAGA
CATTAAATCTGACGTAGAAACAGGCAGCAGCATGAGCGCGGCATTTCGTAAATATCCCCTCTATTTTGATGCTTTATTTT
GCAACTTAATCGGTGCAGGGGAGCAAGCAGGTATTTTAGACACGCTACTTGATCGCCTTGCTACTTATAAAGAAAAAATA
CTCGCTATTAAATCAAAGATTAAGTCAGCTCTTTTCTATCCAATATCAATTATCGTAGTTGCATTTGTGATTGTCGCAGT
GATAATGATTTTTGTGATTCCAGCATTTAAGACACTATTCGATGGGTTTGGCGCAGAGTTACCAGCCCCGACTTTAATTG
TCATGGCGATTTCAGATATTTTTGTGCAATGGTGGTGGGCGATTTTTGGTTCCATTGGATTCGGCATTTGGTTTTTCTTT
TACACATGGAAACGCTCTCTGAGCATGCAAGCCACCATGGATCGATTAATTCTTAAGGTTCCTGTTTTTGGTCCACTAAT
TCGAAAAGCTACAATTGCGCGATGGGCTCGTACTTTAGCGACTATGTTTTCTGCTGGTGTACCTCTTGTAGAAGCCTTAG
ACTCAGTTGCGGGAGCATCTGGCAACCGTGTTTACTATGACGCAACAAAAAAAGTACAGAGTGAAATTAGTACGGGTACA
AGCCTAACTGTTGCTATGCAAAATACCGATGTATTTCCTAATATGGTGCTACAAATGACAGCCATTGGTGAAGAATCTGG
CGCATTGGATGCTATGCTAAGTAAAGTAGCAGATTTTTTTGAAGCTGAAGTTGATGATGCAGTTGATGCACTAGCTAGCT
TGATGGAACCAGTCATCATGGTTGTGCTCGGCGTTCTGATTGGTGGGCTGGTTATCGCCATGTATATGCCTATATTTAAA
ATGGGCTCGGTAGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D7DN47

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.731

99.259

0.533
  pilC Legionella pneumophila strain ERS1305867

53.518

98.272

0.526
  pilC Acinetobacter baumannii D1279779

50.99

99.753

0.509
  pilC Acinetobacter baylyi ADP1

50.882

98.025

0.499
  pilG Neisseria gonorrhoeae MS11

49.38

99.506

0.491
  pilG Neisseria meningitidis 44/76-A

49.132

99.506

0.489
  pilC Vibrio campbellii strain DS40M4

41.895

99.012

0.415
  pilC Vibrio cholerae strain A1552

40.436

100

0.412
  pilC Thermus thermophilus HB27

37.717

99.506

0.375

Multiple sequence alignment