Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M301_RS03100 Genome accession   NC_014207
Coordinates   663158..664876 (-) Length   572 a.a.
NCBI ID   WP_013147303.1    Uniprot ID   D7DN48
Organism   Methylotenera versatilis 301     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 658158..669876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M301_RS03080 (M301_0599) - 658631..659800 (+) 1170 WP_013147299.1 FAD-dependent oxidoreductase -
  M301_RS03085 (M301_0600) - 659797..661005 (+) 1209 WP_013147300.1 FAD-dependent monooxygenase -
  M301_RS03090 (M301_0601) - 661079..661807 (+) 729 WP_013147301.1 DsbC family protein -
  M301_RS03095 (M301_0602) pilC 661926..663143 (-) 1218 WP_013147302.1 type II secretion system F family protein Machinery gene
  M301_RS03100 (M301_0603) pilB 663158..664876 (-) 1719 WP_013147303.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M301_RS03105 (M301_0604) - 665214..665417 (+) 204 WP_013147304.1 cold-shock protein -
  M301_RS03110 (M301_0605) icd 665526..666782 (-) 1257 WP_013147305.1 NADP-dependent isocitrate dehydrogenase -
  M301_RS03115 (M301_0606) - 666828..667379 (+) 552 WP_013147306.1 pseudouridine synthase -
  M301_RS03120 (M301_0607) - 667407..668117 (+) 711 WP_041359733.1 type 1 glutamine amidotransferase -
  M301_RS03125 (M301_0608) aroC 668165..669259 (+) 1095 WP_013147308.1 chorismate synthase -
  M301_RS03130 (M301_0609) - 669390..669626 (+) 237 WP_013147309.1 YdcH family protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62723.12 Da        Isoelectric Point: 4.9693

>NTDB_id=37382 M301_RS03100 WP_013147303.1 663158..664876(-) (pilB) [Methylotenera versatilis 301]
MANAPTSNKLSGLARTLIQEKLLSEDEANAIQAQATSVKNPFISQLITSRKLTAQVIAEASAKAFGFPYFNLDAFNPDYL
PAKKIDTKLMQTNRVLALQVRNNILYVAISDPTNLHALDSVQFQMGMALSPVVVEDDKLGRWIDKISEASDTSMKSMSLD
DDYDLGGDDVASVEDEVQTQEVDDAPVVKFLNKMLLDAINMGASDLHFEPYEKFYRVRYRVDGILREITQPPLAIKEKLA
SRIKVISSMDISEKRIPQDGRMKLVLSKTRTIDFRVSTLPLINGEKIVMRILDPSSATLGIEALGYEPVQKERLLSAVSR
PYGLVLVTGPTGSGKTVSLYTCLNILNSPDVNIATAEDPCEIPLAGINQVNVNDKQGLTFAAALKSFLRQDPDIIMIGEI
RDLETADMAIKAASTGHMVLSTLHTNDAPSTLTRLLNMGVAPFNIASAVSLITAQRLARRLCKNCKVPLDVPKEALYGVG
FTEDDFKDEWQLYGPKEGGCELCNNGYKGRVGIYQVMPITDAISRIIMKSGTAHDIADQAKLEGVKDLRQSGLMKVMQGL
TSIEEVEACTNE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=37382 M301_RS03100 WP_013147303.1 663158..664876(-) (pilB) [Methylotenera versatilis 301]
ATGGCCAATGCACCTACATCAAACAAACTAAGCGGCCTTGCCCGCACATTAATCCAAGAAAAGTTACTTTCTGAGGATGA
AGCGAATGCCATACAAGCGCAAGCAACCTCTGTTAAGAACCCATTTATTTCCCAGCTTATTACCAGTAGGAAGTTAACCG
CCCAAGTCATTGCCGAAGCATCGGCAAAAGCATTTGGTTTCCCCTACTTTAACTTAGATGCATTTAACCCAGATTACCTC
CCAGCTAAAAAAATTGATACTAAACTCATGCAAACCAATCGCGTGTTGGCATTGCAAGTACGTAACAATATTTTGTATGT
TGCAATCTCAGATCCTACTAATTTACATGCATTAGACAGCGTTCAGTTTCAAATGGGCATGGCTTTATCACCGGTCGTCG
TTGAAGATGACAAGCTGGGGCGCTGGATAGATAAAATTTCTGAAGCTAGTGACACCAGCATGAAGTCTATGAGTCTTGAT
GACGATTATGACTTAGGCGGTGATGATGTAGCATCAGTCGAAGATGAAGTGCAGACACAAGAAGTTGATGATGCTCCTGT
TGTTAAGTTTCTAAATAAAATGTTGTTAGATGCTATCAATATGGGGGCGTCTGACTTGCACTTTGAGCCGTACGAGAAAT
TTTACCGCGTACGCTATCGTGTAGATGGCATACTTAGGGAAATCACACAGCCACCACTCGCCATCAAGGAAAAATTAGCT
TCGCGGATTAAAGTTATTTCCAGCATGGACATTTCTGAAAAACGCATCCCACAAGATGGGCGTATGAAGCTGGTGTTATC
CAAAACGCGAACCATCGATTTCCGTGTCAGCACATTGCCGCTTATCAATGGCGAAAAAATCGTGATGCGTATTTTAGATC
CATCAAGCGCCACATTAGGTATTGAGGCGCTTGGCTACGAGCCAGTTCAAAAAGAACGTCTACTAAGCGCTGTCAGTCGT
CCGTATGGACTAGTATTGGTAACAGGGCCTACGGGTTCTGGCAAAACAGTATCGCTGTACACCTGCCTGAATATATTAAA
TAGTCCTGATGTGAACATCGCGACAGCGGAAGATCCATGCGAGATTCCATTAGCTGGCATCAACCAAGTCAACGTAAATG
ATAAGCAAGGGTTAACCTTTGCCGCTGCATTAAAATCATTTCTTCGCCAAGATCCTGACATCATCATGATTGGTGAGATT
CGTGATTTAGAAACTGCGGACATGGCAATTAAAGCCGCGTCTACAGGCCATATGGTATTGTCTACGCTACATACCAATGA
TGCGCCCTCAACATTGACACGTTTACTTAATATGGGCGTTGCACCATTCAATATTGCATCAGCTGTTTCACTGATTACAG
CACAACGCTTAGCAAGACGTTTGTGCAAAAACTGTAAAGTTCCTTTAGACGTTCCAAAAGAAGCTTTATACGGTGTGGGC
TTCACTGAAGACGACTTTAAAGATGAGTGGCAACTATACGGCCCCAAAGAAGGTGGTTGCGAACTATGTAACAACGGCTA
CAAAGGCCGAGTCGGCATTTATCAAGTGATGCCCATTACTGACGCGATTAGCCGTATTATTATGAAAAGCGGAACCGCAC
ATGACATAGCGGATCAAGCAAAACTTGAAGGCGTAAAGGATTTGCGCCAATCGGGTTTAATGAAGGTAATGCAAGGTTTA
ACTTCGATCGAGGAAGTCGAAGCATGTACGAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-149)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D7DN48

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.052

100

0.552
  pilB Acinetobacter baylyi ADP1

54.181

100

0.544
  pilF Neisseria gonorrhoeae MS11

51.95

98.601

0.512
  pilB Legionella pneumophila strain ERS1305867

50.347

100

0.507
  pilB Vibrio cholerae strain A1552

46.018

98.776

0.455
  pilB Vibrio parahaemolyticus RIMD 2210633

45.15

99.126

0.448
  pilB Vibrio campbellii strain DS40M4

44.444

99.126

0.441
  pilF Thermus thermophilus HB27

38.028

99.301

0.378

Multiple sequence alignment