Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E3226_RS00845 Genome accession   NZ_CP041713
Coordinates   141742..142890 (+) Length   382 a.a.
NCBI ID   WP_187356874.1    Uniprot ID   -
Organism   Legionella geestiana strain HL-0438-4026     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 136742..147890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3226_RS00830 (E3226_000545) - 137723..138214 (-) 492 WP_135842101.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  E3226_RS00835 (E3226_000550) - 138193..138858 (-) 666 WP_135842102.1 uracil-DNA glycosylase -
  E3226_RS00840 (E3226_000555) vpdC 138858..141623 (-) 2766 WP_135842103.1 Dot/Icm T4SS effector VpdC -
  E3226_RS00845 (E3226_000560) pilB 141742..142890 (+) 1149 WP_187356874.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  E3226_RS00850 (E3226_000565) - 142883..144100 (+) 1218 WP_135842105.1 type II secretion system F family protein -
  E3226_RS00855 (E3226_000570) - 144103..144969 (+) 867 WP_028387373.1 A24 family peptidase -
  E3226_RS00860 (E3226_000575) - 145042..146238 (+) 1197 WP_028387374.1 MltA domain-containing protein -
  E3226_RS00865 (E3226_000580) - 146242..147606 (+) 1365 WP_135842106.1 MATE family efflux transporter -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 41638.23 Da        Isoelectric Point: 6.4984

>NTDB_id=373344 E3226_RS00845 WP_187356874.1 141742..142890(+) (pilB) [Legionella geestiana strain HL-0438-4026]
MIDALMDGHDADVVQQVETLLTSALSMNASDIHVEPFQDGLRVRFRIDGLLREIVTLPATLAPRVVSRLKIMSNLDISEK
RLPQDGRYRLQPANEKPADFRVSTCPVIHGEKVVLRLLEQGNASLPLDALGMLPEQLALLERVLSRPQGLVLVTGPTGSG
KTITLYAALVRLNAVMRNIATVEDPVEILLNGINQVAINPKTGLDFATVLRALLRQDPDVLMIGEIRDRETAEIAVKAAH
TGHLVLSTLHTTSAAESLVRLQHMGIAAWLLATSLSLVIAQRLVRVLCVHCRIPDTEGTFKAQGCSRCNQGYQGRTGIFE
VLNLDDTLRDAILRGDNTLKLQALSRECGMFSLNKSGTILVRAGVTTAEELYRVTGEEAADA

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=373344 E3226_RS00845 WP_187356874.1 141742..142890(+) (pilB) [Legionella geestiana strain HL-0438-4026]
ATGATAGACGCCCTCATGGATGGTCATGACGCGGATGTGGTGCAACAGGTTGAAACCCTGCTCACTTCTGCGCTTTCGAT
GAATGCCTCCGATATTCACGTTGAACCCTTTCAGGATGGATTGCGCGTACGTTTTCGCATTGATGGCCTGCTGCGTGAAA
TTGTCACCCTGCCGGCAACACTCGCGCCACGCGTAGTTTCCCGGCTTAAGATAATGTCAAACCTTGATATTTCCGAAAAA
CGCCTGCCTCAGGATGGGCGCTACCGTTTGCAGCCTGCAAATGAAAAACCAGCGGATTTTCGTGTCAGTACCTGTCCCGT
GATTCATGGTGAAAAAGTCGTTCTGAGGCTCCTTGAACAGGGGAACGCCAGCCTGCCGCTGGATGCCCTTGGCATGCTTC
CAGAACAGCTTGCGCTGTTAGAGCGCGTACTTTCAAGACCACAGGGGCTTGTACTCGTAACGGGCCCCACCGGCAGTGGC
AAAACCATTACGCTTTATGCCGCTCTCGTTCGGTTAAATGCCGTAATGCGCAACATCGCAACGGTTGAAGATCCGGTAGA
AATTCTCCTGAATGGCATTAATCAAGTCGCGATTAACCCAAAGACTGGCCTCGATTTCGCCACCGTACTGCGCGCGCTGC
TGCGCCAGGACCCGGATGTGCTGATGATAGGAGAAATTCGTGACCGTGAAACTGCTGAGATTGCTGTCAAAGCGGCGCAC
ACCGGGCATCTTGTGCTCTCCACGCTGCACACCACAAGTGCTGCTGAATCGCTGGTTCGTCTGCAGCACATGGGCATTGC
AGCATGGCTTTTGGCGACATCCTTAAGTCTTGTGATTGCGCAGCGTCTGGTACGTGTGCTTTGCGTGCACTGTCGAATAC
CAGACACCGAGGGGACATTTAAAGCCCAGGGGTGCAGCCGGTGCAATCAGGGGTATCAGGGACGAACCGGTATTTTTGAA
GTGCTGAACCTCGATGACACACTGCGAGACGCTATTCTCAGGGGTGATAATACCCTCAAACTGCAGGCGCTATCACGCGA
GTGCGGCATGTTTTCTCTTAACAAGTCCGGTACCATACTCGTGCGGGCGGGGGTAACGACTGCCGAGGAGCTCTACCGTG
TCACGGGTGAGGAAGCCGCTGATGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(20-286)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50.909

100

0.513
  pilB Acinetobacter baumannii D1279779

49.744

100

0.508
  pilB Acinetobacter baylyi ADP1

50.129

100

0.508
  pilB Vibrio campbellii strain DS40M4

49.737

99.476

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

49.474

99.476

0.492
  pilB Vibrio cholerae strain A1552

49.344

99.738

0.492
  pilF Neisseria gonorrhoeae MS11

45.736

100

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

42.476

100

0.458
  pilB Haemophilus influenzae Rd KW20

46.739

96.335

0.45
  pilB Haemophilus influenzae 86-028NP

45.946

96.859

0.445
  pilF Thermus thermophilus HB27

43.85

97.906

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.969

100

0.424
  ctsE Campylobacter jejuni subsp. jejuni 81-176

42.162

96.859

0.408
  pilB Glaesserella parasuis strain SC1401

48.875

81.414

0.398

Multiple sequence alignment